I. V. Mokrousov, S. N. Zhdanova, V. Valcheva, G. I. Alekseeva, M. K. Vinokurova, N. E. Evdokimova, O. B. Ogarkov
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引用次数: 0
Abstract—
The S genotype (first described in Sicily, hence the name) is a part of the Euro-American lineage of Mycobacterium tuberculosis. Here, we report a method for its detection based on PCR-RFLP of the Rv0557 gene. The method was optimized on strains with known whole genome sequences and further applied to screening the DNA collections from Russia, Bulgaria, China, Japan, and Vietnam. PCR-RFLP analysis confirmed the S genotype in strains with the spoligotypes SIT34 (ancestral to S) and SIT1253 (Yakutia strains). Strains from Bulgaria with abridged spoligoprofiles SIT4 and SIT125 were also assigned to the S genotype. The developed method provides for rapid detection of the M. tuberculosis S genotype and reliably resolves phylogenetic ambiguity of the disputed spoligotypes.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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