Liposome Nanoparticles Loaded with Paeoniflorin Protect Neuronal Damage in Parkinson’s Disease by Regulating miR-135a

Abstract

Paeoniflorin (PAE) is an active ingredient extracted from peony. This study aimed to explore the mechanism by which liposome nanoparticles loaded with PAE protect neuronal damage in Parkinson’s disease. Model group, PAE group, PAE-Lips group, PAE-Lips+miR-135a agonist group, PAE-Lips+miR-135a inhibitor group, PAE-Lips+BAY11-7085 group, PAE-Lips+SC75741 group were designed. PCR, learning and memory ability testing, pole climbing test, etc. were used to determine the mechanism of PAE-Lips on Parkinson’s disease and whether it exerts effects through regulating miR-135a. PAE-Lips were successfully constructed. PAE-Lips improved Parkinson’s disease in rats and had a certain connection with miR-135a. Up-regulating miR-135a inhibited NF-κB pathway to a certain extent and improved Parkinson’s disease. It helped protect neurons. Further verification using PAE-Lips+miR-135a agonists, SC75741, BAY11-7085, etc. showed that PAE-Lips upregulated the expression of miR-135 and inhibited NF-κB pathway, which has a good protective effect on neurons in Parkinson’s disease. PAE-Lips can promote miR-135a to inhibit the NF-κB pathway, thereby protecting neuronal damage in Parkinson’s disease. This study will provide a new idea for the prevention and treatment of Parkinson’s disease, clarify the impact of PAE-Lips, miR-135a, NF-κB, BAY11-7085 and SC75741 on Parkinson’s disease, and provide a basis for the combined use of these interventions. The possibility of treating Parkinson’s disease more effectively deserves further exploration and research and provides a theoretical basis for the development of related therapeutic drugs.