Minjing Li, Yueming Liang, Yeliang Wen, Yaofeng Chen, Jian Liu
This study investigated the role of miR-146a in lung fibrosis, specifically focusing on idiopathic pulmonary fibrosis (IPF), which is characterized by excessive alveolar fibrosis and collagen synthesis. The study aimed to explore the impact and mechanism of miR-146a on lung fibrosis using in vitro methods. Human lung fibroblasts (LFs) were transfected with miR-146a mimics and inhibitors to examine their effects. Transforming growth factor-β (TGF-β) was used to activate LFs for 24 hours, while phosphate-buffered saline (PBS)-treated cells served as the control group. The transfection efficiency, level of LFs activation, collagen expression, and cell viability were investigated. The results demonstrated that administration of miR-146a mimics attenuated LFs activation and reduced collagen levels by inhibiting the expression of EGR1, an important factor involved in cell proliferation and differentiation that is positively associated with fibrogenesis. On the other hand, miR-146a inhibitor increased EGR1 expression, but did not significantly affect LFs activation and collagen expression. Furthermore, rescuing EGR1 expression reversed the decrease in LFs activation and collagen expression induced by increased levels of miR-146a. These findings indicate that miR-146a overexpression has an anti-fibrotic effect on LFs by inhibiting EGR1 expression, thereby restraining cell activation and reducing collagen deposition. Therefore, miR-146a holds promise as a potential therapeutic target for mitigating lung fibrosis diseases.
{"title":"miR-146a-Modified Lung Fibroblast Attenuates Transforming Growth Factor Beta-Induced Fibrosis Progress via Downregulation of Early Growth Response Factor 1","authors":"Minjing Li, Yueming Liang, Yeliang Wen, Yaofeng Chen, Jian Liu","doi":"10.1166/jbn.2023.3677","DOIUrl":"https://doi.org/10.1166/jbn.2023.3677","url":null,"abstract":"This study investigated the role of miR-146a in lung fibrosis, specifically focusing on idiopathic pulmonary fibrosis (IPF), which is characterized by excessive alveolar fibrosis and collagen synthesis. The study aimed to explore the impact and mechanism of miR-146a on lung fibrosis\u0000 using in vitro methods. Human lung fibroblasts (LFs) were transfected with miR-146a mimics and inhibitors to examine their effects. Transforming growth factor-β (TGF-β) was used to activate LFs for 24 hours, while phosphate-buffered saline (PBS)-treated cells\u0000 served as the control group. The transfection efficiency, level of LFs activation, collagen expression, and cell viability were investigated. The results demonstrated that administration of miR-146a mimics attenuated LFs activation and reduced collagen levels by inhibiting the expression of\u0000 EGR1, an important factor involved in cell proliferation and differentiation that is positively associated with fibrogenesis. On the other hand, miR-146a inhibitor increased EGR1 expression, but did not significantly affect LFs activation and collagen expression. Furthermore, rescuing EGR1\u0000 expression reversed the decrease in LFs activation and collagen expression induced by increased levels of miR-146a. These findings indicate that miR-146a overexpression has an anti-fibrotic effect on LFs by inhibiting EGR1 expression, thereby restraining cell activation and reducing collagen\u0000 deposition. Therefore, miR-146a holds promise as a potential therapeutic target for mitigating lung fibrosis diseases.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138613013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study assessed the effect of miR-200b liposome nanoparticles in restraining chemotherapy resistance in rats with breast cancer through induction of epidermal growth factor receptor enzyme-linked immunosorbent assay enzyme-linked immunosorbent assay (EGFR)/extracellular regulated protein kinases (ERK) signal pathway. 50 rats were divided into 4 sets, which included control set, empty carrier set, miR-200b set, and set of miR-200b packaged with liposome nanoparticles. The liposome nanoparticles were prepared and identified, and drug-resistant breast cancer cells were observed and identified. The growth inhibition ratio, miR-200b expression, drug-resistance, growth curve, drug resistance of cells, EGFR and ERK protein expressions were observed. miR-200b expression in the et of miR-200b packaged with liposome nanoparticles was highest, second in the miR-200b set and empty carrier set, and lowest in the control set. The IC50 value in the miR-200b packaged with liposome nanoparticles was highest. The absorbance in the set of miR-200b liposome nanoparticles was lowest. The immunofluorescence (IF) strength of miR-200b in the miR-200b liposome nanoparticles was highest. The EGFR and ERK protein expressions, and levels of pEGFR and p-ERK in the miR-200b liposome nanoparticles set was highest. In conclusion, chemotherapy resistance of breast cancer cells could be restrained by miR-200b liposome nanoparticles through restraining of the EGFR/ERK signal pathway.
{"title":"Effect of miR-200b Liposome Nanoparticles on Chemotherapy Resistance in Rats with Breast Cancer Through Induction of Epidermal Growth Factor Receptor/Extracellular Signal-Regulated Kinase Signal Pathway","authors":"Sirui Li, Conghui Li, Xiaosong Ma, Xuyang Zhang, Liangyu Zhang","doi":"10.1166/jbn.2023.3691","DOIUrl":"https://doi.org/10.1166/jbn.2023.3691","url":null,"abstract":"This study assessed the effect of miR-200b liposome nanoparticles in restraining chemotherapy resistance in rats with breast cancer through induction of epidermal growth factor receptor enzyme-linked immunosorbent assay enzyme-linked immunosorbent assay (EGFR)/extracellular regulated\u0000 protein kinases (ERK) signal pathway. 50 rats were divided into 4 sets, which included control set, empty carrier set, miR-200b set, and set of miR-200b packaged with liposome nanoparticles. The liposome nanoparticles were prepared and identified, and drug-resistant breast cancer cells were\u0000 observed and identified. The growth inhibition ratio, miR-200b expression, drug-resistance, growth curve, drug resistance of cells, EGFR and ERK protein expressions were observed. miR-200b expression in the et of miR-200b packaged with liposome nanoparticles was highest, second in the miR-200b\u0000 set and empty carrier set, and lowest in the control set. The IC50 value in the miR-200b packaged with liposome nanoparticles was highest. The absorbance in the set of miR-200b liposome nanoparticles was lowest. The immunofluorescence (IF) strength of miR-200b in the miR-200b liposome nanoparticles\u0000 was highest. The EGFR and ERK protein expressions, and levels of pEGFR and p-ERK in the miR-200b liposome nanoparticles set was highest. In conclusion, chemotherapy resistance of breast cancer cells could be restrained by miR-200b liposome nanoparticles through restraining of the EGFR/ERK\u0000 signal pathway.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"24 25","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138624380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to compare the effects of nasal endoscopic bipolar electrocoagulation (BE) and low-temperature plasma knife (LPK) treatment on intractable epistaxis (IE) in order to provide a reference and guidance for future treatment options for IE. A total of 109 patients with IE admitted to Dongying People’s Hospital from June 2021 to May 2022 were selected for the study. Of these, 58 were treated with BE (BE group) and 51 were treated with LPK (LPK group). The nasal cavities of patients in the BE and LPK groups were filled with a gelatin sponge and calcium alginate dressing, respectively, after surgery. The clinical efficacy, operation time, hemostasis time, and intraoperative bleeding volume of the two groups were compared. Patients’ unilateral inspiratory resistance (IR), expiratory resistance (ER), bilateral total inspiratory resistance (TIR), and total expiratory resistance (TER) were measured using a nasal resistance tester, and pain was assessed using a visual analog scale (VAS). Serum epinephrine (E), norepinephrine (NE), and cortisol (Cor) levels were also measured in patients before and after surgery, and complications were recorded. The recurrence of IE was measured at a 6-month follow-up after discharge from the hospital. There were no differences in clinical efficacy, hemostasis time, or intraoperative bleeding between groups (P >0.05), and the operative time was longer in both BE groups (P <0.05). In addition, postoperative IR, ER, TIR, and TER were higher in the BE group than in the LPK group, and the VAS score results were also higher at 1 d postoperatively (P <0.05). After surgery, E, NE, and Cor were elevated in both groups; more so in the BE group than in the LPK group (P <0.05). Differences in the incidence of complications and prognostic recurrence rates between groups were not statistically significant (P >0.05).
{"title":"Effectiveness of Calcium Alginate Dressing in Combination with Nasal Endoscopic Bipolar Electrocoagulation and Low-Temperature Plasma Knife Treatment on Bleeding Volume, Nasal Ventilation, Stress Response, and Recurrence Rate in Patients with Refractory Epistaxis","authors":"Yi Su, Xinye Guo, Yan Nie","doi":"10.1166/jbn.2023.3724","DOIUrl":"https://doi.org/10.1166/jbn.2023.3724","url":null,"abstract":"The aim of this study was to compare the effects of nasal endoscopic bipolar electrocoagulation (BE) and low-temperature plasma knife (LPK) treatment on intractable epistaxis (IE) in order to provide a reference and guidance for future treatment options for IE. A total of 109 patients\u0000 with IE admitted to Dongying People’s Hospital from June 2021 to May 2022 were selected for the study. Of these, 58 were treated with BE (BE group) and 51 were treated with LPK (LPK group). The nasal cavities of patients in the BE and LPK groups were filled with a gelatin sponge and\u0000 calcium alginate dressing, respectively, after surgery. The clinical efficacy, operation time, hemostasis time, and intraoperative bleeding volume of the two groups were compared. Patients’ unilateral inspiratory resistance (IR), expiratory resistance (ER), bilateral total inspiratory\u0000 resistance (TIR), and total expiratory resistance (TER) were measured using a nasal resistance tester, and pain was assessed using a visual analog scale (VAS). Serum epinephrine (E), norepinephrine (NE), and cortisol (Cor) levels were also measured in patients before and after surgery, and\u0000 complications were recorded. The recurrence of IE was measured at a 6-month follow-up after discharge from the hospital. There were no differences in clinical efficacy, hemostasis time, or intraoperative bleeding between groups (P >0.05), and the operative time was longer in both\u0000 BE groups (P <0.05). In addition, postoperative IR, ER, TIR, and TER were higher in the BE group than in the LPK group, and the VAS score results were also higher at 1 d postoperatively (P <0.05). After surgery, E, NE, and Cor were elevated in both groups; more so in the\u0000 BE group than in the LPK group (P <0.05). Differences in the incidence of complications and prognostic recurrence rates between groups were not statistically significant (P >0.05).","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"47 12","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138626799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Because of its exceptional biocompatibility and bioactivity, decellularized extracellular matrix (dECM) has attracted the interest of researchers in tissue regeneration and wound recovery. However, the processibility of such biomaterial remains a great challenge in tissue engineering. Herein, porcine decellularized skin matrix (pDSM) was enzymatically digested into a pDSM-sol, which was then mechanically mixed with micron-sized short pDSM fibers resulted from wet electrospinning, to eventually achieve a reinforced hybrid hydrogel with interpenetrating nano- and microstructures. The physical properties of this hybrid hydrogel were evaluated by varying the concentration of pDSM-sol, composition of the gel/fiber contents, and the length of short pDSM microfibers. Furthermore, bioscaffolds fabricated by such dual-scale nanofibrous hydrogels were tested in both in vitro laboratory conditions and in vivo living systems to evaluate their proficiency in wound repair. It’s noteworthy that the incorporation of short fibers led to the acceleration of the sol–gel transition, resulting in a significant enhancement of the hybrid hydrogel’s storage modulus, coupled with a reduction in its degradation rate. This hybrid hydrogel, co-cultured separately with human umbilical vein endothelial cells (HUVECs) and RAW264.7 cells, can promote the secretion of vascular endothelial growth factor (VEGF) from both cell types. Additionally, it facilitates the secretion of M2 phenotype characteristic proteins from RAW264.7 cells. Finally, the implantation of hybrid hydrogel scaffolds led to highly facilitated regeneration effects, including wound healing, collagen deposition, suppression of inflammation, and angiogenesis in a skin-defected rat model. These promising results indicate that such hybrid hydrogels with hierarchical micro- and nanofibrous structures have shown great application potential in future regenerative medicine.
{"title":"A Hierarchically Micro- and Nanofibrous Hybrid Hydrogel Derived from Decellularized Skin Matrix with High Bioactivity and Tunable Mechanical Properties for Accelerated Wound Healing","authors":"Jiahui Wu, Rongli Deng, Mingsheng Liu, Jiaxin Chen, Ying Bai, Jiang Lu, Jing Zhou, Daping Quan","doi":"10.1166/jbn.2023.3706","DOIUrl":"https://doi.org/10.1166/jbn.2023.3706","url":null,"abstract":"Because of its exceptional biocompatibility and bioactivity, decellularized extracellular matrix (dECM) has attracted the interest of researchers in tissue regeneration and wound recovery. However, the processibility of such biomaterial remains a great challenge in tissue engineering.\u0000 Herein, porcine decellularized skin matrix (pDSM) was enzymatically digested into a pDSM-sol, which was then mechanically mixed with micron-sized short pDSM fibers resulted from wet electrospinning, to eventually achieve a reinforced hybrid hydrogel with interpenetrating nano- and microstructures.\u0000 The physical properties of this hybrid hydrogel were evaluated by varying the concentration of pDSM-sol, composition of the gel/fiber contents, and the length of short pDSM microfibers. Furthermore, bioscaffolds fabricated by such dual-scale nanofibrous hydrogels were tested in both in\u0000 vitro laboratory conditions and in vivo living systems to evaluate their proficiency in wound repair. It’s noteworthy that the incorporation of short fibers led to the acceleration of the sol–gel transition, resulting in a significant enhancement of the hybrid hydrogel’s\u0000 storage modulus, coupled with a reduction in its degradation rate. This hybrid hydrogel, co-cultured separately with human umbilical vein endothelial cells (HUVECs) and RAW264.7 cells, can promote the secretion of vascular endothelial growth factor (VEGF) from both cell types. Additionally,\u0000 it facilitates the secretion of M2 phenotype characteristic proteins from RAW264.7 cells. Finally, the implantation of hybrid hydrogel scaffolds led to highly facilitated regeneration effects, including wound healing, collagen deposition, suppression of inflammation, and angiogenesis in a\u0000 skin-defected rat model. These promising results indicate that such hybrid hydrogels with hierarchical micro- and nanofibrous structures have shown great application potential in future regenerative medicine.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138609813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In duck breeding, the growth and development of skeletal muscle is an important factor influencing the meat production performance of ducks. Therefore, the determination of Myod and Myf5 gene expression in poultry skeletal muscle tissues can help to understand the muscle development of poultry, improve the production performance and feed conversion rate of animal organism, enhance the rapid protein deposition in animal organism, and obtain high quality and quantity of animal livestock products. In this study, a fluorescent PCR assay system for Myod and Myf5 genes was developed, and a dual integrated rapid detection platform suitable for detecting Myod and Myf5 genes in poultry muscle tissue with a sensitivity of 10 copies/μL was constructed using a set of commercial, fully automated nucleic acid analyzer with integrated detection based on magnetic bead method for nucleic acid extraction and PCR fluorescence detection. For 20 simulated samples, the integrated detection system was consistent with the results of qPCR experiments after conventional laboratory extraction, while the closed cassette-based detection reduced the chance of contamination occurrence, making the results more reliable and accurate, which is ideal for immediate on-site rapid detection.
{"title":"A Rapid Integrated Detection Platform for Genes Related to Duck Muscle Tissue Development","authors":"Zhiming Zhu, Li Li, Zhongwei Miao, Qingwu Xin, Linli Zhang, Qinlou Huang, Nengzhu Zheng","doi":"10.1166/jbn.2023.3728","DOIUrl":"https://doi.org/10.1166/jbn.2023.3728","url":null,"abstract":"In duck breeding, the growth and development of skeletal muscle is an important factor influencing the meat production performance of ducks. Therefore, the determination of Myod and Myf5 gene expression in poultry skeletal muscle tissues can help to understand the muscle\u0000 development of poultry, improve the production performance and feed conversion rate of animal organism, enhance the rapid protein deposition in animal organism, and obtain high quality and quantity of animal livestock products. In this study, a fluorescent PCR assay system for Myod\u0000 and Myf5 genes was developed, and a dual integrated rapid detection platform suitable for detecting Myod and Myf5 genes in poultry muscle tissue with a sensitivity of 10 copies/μL was constructed using a set of commercial, fully automated nucleic acid analyzer\u0000 with integrated detection based on magnetic bead method for nucleic acid extraction and PCR fluorescence detection. For 20 simulated samples, the integrated detection system was consistent with the results of qPCR experiments after conventional laboratory extraction, while the closed cassette-based\u0000 detection reduced the chance of contamination occurrence, making the results more reliable and accurate, which is ideal for immediate on-site rapid detection.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" 21","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138613720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Superparamagnetic iron oxide nanoparticles exert its action on repairing bone defects. Whether they have the same repair effect on osteosarcoma bone defects and the mechanism is worth studying. The bone defect model of osteosarcoma rats was constructed and divided into bone defect group, positive control group, low-dose nano-group, medium-dose nano-group, high-dose nano-group, and blocker group followed by analysis of new bone formation, bone marrow derived mesenchymal stem cells (BMSC) homing, bone morphogenetic protein-2 (BMP-2), Collagen I, and Phosphorylated 44/42 mitogen-activated protein kinase (p44/42) protein expression. The proportion of new bone formation in positive control group and different dose groups was higher than bone defect group and blocker group (P <0.05). The positive control group had rich collagen fibers at repair site, which were more cellulose and neatly arranged. Low-dose group was more collagenous than positive control group. There was no new bone formation in the bone defect group and the blocking agent group and no Y chromosome positive cells were found in the blood vessel wall. New bone formation was seen in the positive control group and the nano-group and the number of blood vessels was abundant and rich in Y chromosome positive cells. Compared with the other two groups, positive control group and nano-group had higher BMP-2, Collagen I expression (P <0.05), and lower p44/42 expression (P <0.05). The p-mitogen-activated protein kinase (MAPK) levels were the lowest in bone defect group, blocker group, and positive control group, while nano-group was opposite. Superparamagnetic iron oxide nanoparticles can repair bone defects in osteosarcoma rats, and the mechanism of action is mainly related to the activation of the MAPK pathway.
{"title":"Superparamagnetic Iron Oxide Nanoparticles Activate Mitogen-Activated Protein Kinase Pathway and Promote Bone Marrow Derived Mesenchymal Stem Cells Homing to Repair Bone Defects in Rats with Osteosarcoma","authors":"Xiang Wang, Xiaochuan Dong, Huabin Wang, Zhengkai Xiang","doi":"10.1166/jbn.2023.3723","DOIUrl":"https://doi.org/10.1166/jbn.2023.3723","url":null,"abstract":"Superparamagnetic iron oxide nanoparticles exert its action on repairing bone defects. Whether they have the same repair effect on osteosarcoma bone defects and the mechanism is worth studying. The bone defect model of osteosarcoma rats was constructed and divided into bone defect group,\u0000 positive control group, low-dose nano-group, medium-dose nano-group, high-dose nano-group, and blocker group followed by analysis of new bone formation, bone marrow derived mesenchymal stem cells (BMSC) homing, bone morphogenetic protein-2 (BMP-2), Collagen I, and Phosphorylated 44/42 mitogen-activated\u0000 protein kinase (p44/42) protein expression. The proportion of new bone formation in positive control group and different dose groups was higher than bone defect group and blocker group (P <0.05). The positive control group had rich collagen fibers at repair site, which were more\u0000 cellulose and neatly arranged. Low-dose group was more collagenous than positive control group. There was no new bone formation in the bone defect group and the blocking agent group and no Y chromosome positive cells were found in the blood vessel wall. New bone formation was seen in the positive\u0000 control group and the nano-group and the number of blood vessels was abundant and rich in Y chromosome positive cells. Compared with the other two groups, positive control group and nano-group had higher BMP-2, Collagen I expression (P <0.05), and lower p44/42 expression (P\u0000 <0.05). The p-mitogen-activated protein kinase (MAPK) levels were the lowest in bone defect group, blocker group, and positive control group, while nano-group was opposite. Superparamagnetic iron oxide nanoparticles can repair bone defects in osteosarcoma rats, and the mechanism of action\u0000 is mainly related to the activation of the MAPK pathway.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138615340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Naiwei Li, Dinesh Kumar Sain, Hadi Jabbari, Somayeh Vafaei, Guluzar Ozbolat, Ramirez-Coronel Andres Alexis, Akhavan-Sigari Reza
In the current study, graphene oxide (GO) as raw material synthesized using different reduction degrees prepared by a mild method. We used Fourier transform infrared spectroscopy (FTIR), Raman Spectroscopy (Raman), X-ray photoelectron spectroscopy (XPS), ultraviolet-visible spectroscopy (UV-Vis), transmission electron microscopy (TEM) and EDS spectroscopy to characterize its structure and morphology. The results of cell experiments showed the significant cytotoxicity activity of reduced GO under laser irradiation. In addition, reduced GO kills tumor cells through the dual effects of photothermal and phototoxicity under near-infrared (NIR) laser irradiation and can be used as photothermal therapy
本研究以氧化石墨烯（GO）为原料，采用不同还原度的温和方法合成制备。我们使用傅立叶变换红外光谱（FTIR）、拉曼光谱（Raman Spectroscopy）、X射线光电子能谱（XPS）、紫外可见光谱（UV-Vis）、透射电子显微镜（TEM）和EDS光谱来表征其结构和形态。细胞实验结果表明，还原型 GO 在激光照射下具有显著的细胞毒性活性。此外，在近红外激光照射下，还原型 GO 可通过光热和光毒双重效应杀死肿瘤细胞，可用作光热疗法
{"title":"Graphene Oxide Synthesized Using Mild Reduction Method Improved the Anti-Tumor Efficacy on Human Cervical Cancer","authors":"Naiwei Li, Dinesh Kumar Sain, Hadi Jabbari, Somayeh Vafaei, Guluzar Ozbolat, Ramirez-Coronel Andres Alexis, Akhavan-Sigari Reza","doi":"10.1166/jbn.2023.3725","DOIUrl":"https://doi.org/10.1166/jbn.2023.3725","url":null,"abstract":"In the current study, graphene oxide (GO) as raw material synthesized using different reduction degrees prepared by a mild method. We used Fourier transform infrared spectroscopy (FTIR), Raman Spectroscopy (Raman), X-ray photoelectron spectroscopy (XPS), ultraviolet-visible spectroscopy (UV-Vis), transmission electron microscopy (TEM) and EDS spectroscopy to characterize its structure and morphology. The results of cell experiments showed the significant cytotoxicity activity of reduced GO under laser irradiation. In addition, reduced GO kills tumor cells through the dual effects of photothermal and phototoxicity under near-infrared (NIR) laser irradiation and can be used as photothermal therapy","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"92 1-2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139191228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bowen Yang, Luyao Huang, Zishuo Zhou, Shanmei Yin, Mingrong Xi
We aimed to develop an ovarian cancer-directed drug delivery system based on the high affinity of hyaluronic acid for CD44. The effects and mechanisms of hyaluronic acid-containing nanoparticles were investigated. The expression of CD44 in ovarian cancer was also determined. Hyaluronic acid polymerized nanoparticles (HANPs), FITC-HANPs, and paclitaxel (PTX)-HANPs were prepared, and their characteristics were evaluated. The in vitro targetability and cytotoxicity properties of PTX-HANPs were evaluated through in vitro drug uptake and cytotoxicity assays. The mechanisms of PTX-HANPs activity were investigated by apoptosis, wound healing, and Transwell invasion assays. In vivo targeting properties of HANPs were observed using a mouse ID8 subcutaneous model. in vitro experiments revealed an improved uptake of FITC-HANPs. The cytotoxicity of PTX-HANPs in A2780/CP70 and ID8 cells was higher than that of PTX alone. PTX-HANPs increased cell apoptosis in a dose-dependent manner and exhibited a similar ability as PTX to inhibit cell migration. Furthermore, HANPs did not promote A2780/CP70 or ID8 cell migration and showed limited inhibitory effects on their invasion. In vivo drug tracing experiments demonstrated the targetability of FITC-HANPs. In conclusion, PTX-HANPs improved PTX targetability and exhibited potent tumor-specific therapeutic activities. It may be considered a promising formulation for the preclinical development of agents targeting epithelial ovarian cancer.
{"title":"Paclitaxel Loaded Hyaluronic Acid Polymerized Nanoparticles Designed for Ovarian Cancer Therapy","authors":"Bowen Yang, Luyao Huang, Zishuo Zhou, Shanmei Yin, Mingrong Xi","doi":"10.1166/jbn.2023.3509","DOIUrl":"https://doi.org/10.1166/jbn.2023.3509","url":null,"abstract":"We aimed to develop an ovarian cancer-directed drug delivery system based on the high affinity of hyaluronic acid for CD44. The effects and mechanisms of hyaluronic acid-containing nanoparticles were investigated. The expression of CD44 in ovarian cancer was also determined. Hyaluronic\u0000 acid polymerized nanoparticles (HANPs), FITC-HANPs, and paclitaxel (PTX)-HANPs were prepared, and their characteristics were evaluated. The in vitro targetability and cytotoxicity properties of PTX-HANPs were evaluated through in vitro drug uptake and cytotoxicity assays. The\u0000 mechanisms of PTX-HANPs activity were investigated by apoptosis, wound healing, and Transwell invasion assays. In vivo targeting properties of HANPs were observed using a mouse ID8 subcutaneous model. in vitro experiments revealed an improved uptake of FITC-HANPs. The cytotoxicity\u0000 of PTX-HANPs in A2780/CP70 and ID8 cells was higher than that of PTX alone. PTX-HANPs increased cell apoptosis in a dose-dependent manner and exhibited a similar ability as PTX to inhibit cell migration. Furthermore, HANPs did not promote A2780/CP70 or ID8 cell migration and showed limited\u0000 inhibitory effects on their invasion. In vivo drug tracing experiments demonstrated the targetability of FITC-HANPs. In conclusion, PTX-HANPs improved PTX targetability and exhibited potent tumor-specific therapeutic activities. It may be considered a promising formulation for the preclinical\u0000 development of agents targeting epithelial ovarian cancer.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" 6","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138615688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The recognition of lymph node (LN) metastasis is critical for breast cancer staging. Axillary lymph node (ALN) puncture or resection followed by biopsy, to determine whether the presence of metastasis is the diagnostic ‘gold standard’ for axillary lymph node metastasis. This procedure is an invasive procedure that triggers a series of complications. To solve this problem, we developed an ultrasmall superparamagnetic polyacrylic acid-modified iron oxide nanoparticles (PAA@IONs), which exhibit excellent physicochemical characteristics and are extremely stable in the aqueous state. They had an average hydrated particle size of 37.81±0.80 nm, average zeta potential of −38.7±3.8 mV, relaxivity R1 of 25.53±1.58 s−1mM−1, and R2 of 43.10±3.43 s−1mM−1. Animal magnetic resonance imaging (MRI) of the inflammatory hyperplasia model and tumor metastasis model of lymph nodes showed that the samples could effectively detect the metastasized tumors in lymph nodes (n =8). The inflammatory lymphadenopathy did not affect lymph node diagnosis, and this property helped overcome the challenge of current lymph node diagnosis, showing high sensitivity (100%) and specificity (83%). Body weight, hematology, coagulation parameters, serum biochemistry, gross anatomy, and histopathological examination of all Sprague-Dawley (SD) rats after intravenous administration of single or multiple doses of PAA@IONs showed no abnormal findings. Therefore, the ultrasmall superparamagnetic iron oxide nanoparticles constructed herein are a promising contrast agent for nodal tumor staging.
{"title":"Polyacrylic Acid-Modified Superparamagnetic Iron Oxide Nanoparticles Differentiate Between Hyperplastic and Metastatic Breast Cancer Lymph Nodes","authors":"Heping Hu, Guangyi Fu, Zhao Ding, Yiguo Hu, Guilin Luo, Zongning Yin","doi":"10.1166/jbn.2023.3721","DOIUrl":"https://doi.org/10.1166/jbn.2023.3721","url":null,"abstract":"The recognition of lymph node (LN) metastasis is critical for breast cancer staging. Axillary lymph node (ALN) puncture or resection followed by biopsy, to determine whether the presence of metastasis is the diagnostic ‘gold standard’ for axillary lymph node metastasis.\u0000 This procedure is an invasive procedure that triggers a series of complications. To solve this problem, we developed an ultrasmall superparamagnetic polyacrylic acid-modified iron oxide nanoparticles (PAA@IONs), which exhibit excellent physicochemical characteristics and are extremely stable\u0000 in the aqueous state. They had an average hydrated particle size of 37.81±0.80 nm, average zeta potential of −38.7±3.8 mV, relaxivity R1 of 25.53±1.58 s−1mM−1, and R2 of 43.10±3.43 s−1mM−1.\u0000 Animal magnetic resonance imaging (MRI) of the inflammatory hyperplasia model and tumor metastasis model of lymph nodes showed that the samples could effectively detect the metastasized tumors in lymph nodes (n =8). The inflammatory lymphadenopathy did not affect lymph node diagnosis,\u0000 and this property helped overcome the challenge of current lymph node diagnosis, showing high sensitivity (100%) and specificity (83%). Body weight, hematology, coagulation parameters, serum biochemistry, gross anatomy, and histopathological examination of all Sprague-Dawley (SD) rats after\u0000 intravenous administration of single or multiple doses of PAA@IONs showed no abnormal findings. Therefore, the ultrasmall superparamagnetic iron oxide nanoparticles constructed herein are a promising contrast agent for nodal tumor staging.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138611699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Our research aims to evaluate the repairment of graphene/β-tricalcium phosphate (G/β-TCP) composite scaffold on cartilage defect in rabbit knee joints. The G/β-TCP composite material containing BMSCs was surgically implanted into the femoral condyle of a full-thickness cartilage defect model in rabbits. XRD showed that no difference in the X-ray diffraction characteristics was observed between G/β-TCP and β-TCP. The biomechanical test claimed that the graphene doped β-TCP material processed higher mechanical strength. Scanning electron microscopy showed that the surface of G/β-TCP material was smoother and the texture was denser. The scaffold combined with BMSCs was transplanted into the full-thickness cartilage defect rabbit model and the results showed that the serum CRP level increased only 1 month after implantation, and the Cr level increased at 2 months after implantation, while G/β-TCP material showed rare significant pathological changes on the liver, spleen, kidney, brain, and soft tissue around the operation, which indicated a promising biocompatibility. The expression of type I and II collagen in the cartilage tissue of G/β-TCP treated rabbits was dramatically elevated compared to β-TCP at 2 and 3 months after implantation. Collectively, the G/β-TCP composite scaffold facilitated the repairment of cartilage defects in the rabbit knee joints.
{"title":"Graphene/β-Tricalcium Phosphate Composite Scaffold Facilitates the Repairment of Cartilage Defect in Rabbit Knee Joint","authors":"Wendong Huang, Yong Liao, Xiaolu Yuan, Jianhui Huang, Ya Chen, Binxiu Zhao","doi":"10.1166/jbn.2023.3715","DOIUrl":"https://doi.org/10.1166/jbn.2023.3715","url":null,"abstract":"Our research aims to evaluate the repairment of graphene/β-tricalcium phosphate (G/β-TCP) composite scaffold on cartilage defect in rabbit knee joints. The G/β-TCP composite material containing BMSCs was surgically implanted into the femoral condyle\u0000 of a full-thickness cartilage defect model in rabbits. XRD showed that no difference in the X-ray diffraction characteristics was observed between G/β-TCP and β-TCP. The biomechanical test claimed that the graphene doped β-TCP material processed higher mechanical\u0000 strength. Scanning electron microscopy showed that the surface of G/β-TCP material was smoother and the texture was denser. The scaffold combined with BMSCs was transplanted into the full-thickness cartilage defect rabbit model and the results showed that the serum CRP level increased\u0000 only 1 month after implantation, and the Cr level increased at 2 months after implantation, while G/β-TCP material showed rare significant pathological changes on the liver, spleen, kidney, brain, and soft tissue around the operation, which indicated a promising biocompatibility.\u0000 The expression of type I and II collagen in the cartilage tissue of G/β-TCP treated rabbits was dramatically elevated compared to β-TCP at 2 and 3 months after implantation. Collectively, the G/β-TCP composite scaffold facilitated the repairment of cartilage\u0000 defects in the rabbit knee joints.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"4 9","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138623593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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