Guoping Kuang, Ying Li, Zhenghua Li, Yulun Ou, Zhenghua Li
This study analyzes the efficacy of phacoemulsification (PE) plus intraocular lens implantation (IOLI) in patients with complicated cataracts secondary to uveitis. Nanostructured photothermal ring integrated (nano)-intraocular lens (IOLs) were developed to improve the biocompatibility and optical properties of IOLs. However, data on the safety profile and effectiveness of IOLI for patients with complicated cataracts secondary to uveitis are scarce, and studies have questioned the safety of PE in patients undergoing cataract surgery. We used gold nanoparticle (AuNP)-modified commercially available intraocular IOLs combined with PE technology to treat patients with complicated cataracts secondary to uveitis. First, we found that AuNP-modified IOLs were effective in reducing macrophage and bacterial adhesion and were biocompatible with the organism. Then, 64 patients with complicated cataracts secondary to uveitis were totally selected from February 2019 to February 2022, of which 33 cases were treated with PE+IOLI (test group) and 31 with conventional small-incision extracapsular cataract extraction (SIECCE) and IOLI (control group). Intergroup comparisons based on efficacy, safety, visual acuity (VA), aqueous humor protein levels before and after treatment, and quality of life (assessed with the Generic Quality of Life Inventory-74, GQOLI-74) revealed higher overall treatment efficacy and postoperative VA, as well as lower complication rate and postoperative aqueous humor protein levels, in the test group than the control group. Moreover, the test group had higher GQOLI-74 scores in the dimensions of physical function, mental health, psychological health, and social competence than the control group. Thus, PE+IOLI is more suitable for patients with complicated cataracts secondary to uveitis than conventional SIECCE+IOLI. PE+IOLI can improve treatment efficacy and safety, as well as patient VA and quality of life.
本研究分析了超声乳化术（PE）加眼内人工晶体植入术（IOLI）对葡萄膜炎继发复杂性白内障患者的疗效。纳米结构光热环集成（纳米）眼内人工晶体（IOL）的开发旨在改善人工晶体的生物相容性和光学性能。然而，有关人工晶体植入术对继发葡萄膜炎的复杂性白内障患者的安全性和有效性的数据却很少，而且有研究对白内障手术患者使用 PE 的安全性提出了质疑。我们使用金纳米粒子（AuNP）修饰的市售眼内人工晶体结合 PE 技术治疗葡萄膜炎继发的复杂性白内障患者。首先，我们发现 AuNP 改性人工晶体能有效减少巨噬细胞和细菌的粘附，并且与生物体具有生物相容性。然后，在2019年2月至2022年2月期间，完全选取64例继发于葡萄膜炎的复杂性白内障患者，其中33例采用PE+人工晶体植入术（试验组），31例采用常规小切口白内障囊外摘除术（SIECCE）和人工晶体植入术（对照组）。根据疗效、安全性、视力（VA）、治疗前后房水蛋白水平和生活质量（采用通用生活质量量表-74，GQOLI-74 进行评估）进行的组间比较显示，试验组的总体疗效和术后视力均高于对照组，并发症发生率和术后房水蛋白水平也低于对照组。此外，试验组在身体功能、精神健康、心理健康和社交能力方面的 GQOLI-74 得分均高于对照组。因此，PE+人工晶体植入术比传统的SIECCE+人工晶体植入术更适合继发于葡萄膜炎的复杂性白内障患者。PE+IOLI可提高治疗效果和安全性，改善患者的视力和生活质量。
{"title":"Phacoemulsification Plus Intraocular Lens Implantation with Gold Nanoparticles for Complicated Cataract Secondary to Uveitis: Efficacy Analysis","authors":"Guoping Kuang, Ying Li, Zhenghua Li, Yulun Ou, Zhenghua Li","doi":"10.1166/jbn.2024.3847","DOIUrl":"https://doi.org/10.1166/jbn.2024.3847","url":null,"abstract":"This study analyzes the efficacy of phacoemulsification (PE) plus intraocular lens implantation (IOLI) in patients with complicated cataracts secondary to uveitis. Nanostructured photothermal ring integrated (nano)-intraocular lens (IOLs) were developed to improve the biocompatibility\u0000 and optical properties of IOLs. However, data on the safety profile and effectiveness of IOLI for patients with complicated cataracts secondary to uveitis are scarce, and studies have questioned the safety of PE in patients undergoing cataract surgery. We used gold nanoparticle (AuNP)-modified\u0000 commercially available intraocular IOLs combined with PE technology to treat patients with complicated cataracts secondary to uveitis. First, we found that AuNP-modified IOLs were effective in reducing macrophage and bacterial adhesion and were biocompatible with the organism. Then, 64 patients\u0000 with complicated cataracts secondary to uveitis were totally selected from February 2019 to February 2022, of which 33 cases were treated with PE+IOLI (test group) and 31 with conventional small-incision extracapsular cataract extraction (SIECCE) and IOLI (control group). Intergroup comparisons\u0000 based on efficacy, safety, visual acuity (VA), aqueous humor protein levels before and after treatment, and quality of life (assessed with the Generic Quality of Life Inventory-74, GQOLI-74) revealed higher overall treatment efficacy and postoperative VA, as well as lower complication rate\u0000 and postoperative aqueous humor protein levels, in the test group than the control group. Moreover, the test group had higher GQOLI-74 scores in the dimensions of physical function, mental health, psychological health, and social competence than the control group. Thus, PE+IOLI is more suitable\u0000 for patients with complicated cataracts secondary to uveitis than conventional SIECCE+IOLI. PE+IOLI can improve treatment efficacy and safety, as well as patient VA and quality of life.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141230076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Porphyromonas gingivalis (P. gingivalis) constitutes an essential part of the subgingival dental plaque biofilm, serving as a significant factor in the development of periodontitis. Therefore, establishing a rapid and highly sensitive detection method for P. gingivalis is crucial to effectively manage periodontitis and its associated complications. In this study, droplet digital PCR (ddPCR) technology was employed for the detection of P. gingivalis, with a detection limit of 101 CFU/mL, exhibiting a 10-fold higher sensitivity compared to qPCR (with a sensitivity of 102 CFU/mL). Furthermore, no cross-reactivity was observed with four other bacterial species. In comparison to real-time quantitative PCR, ddPCR demonstrated enhanced sensitivity in detecting P. gingivalis at lower concentrations in 16 simulated samples, indicating its applicability for rapid detection of P. gingivalis.
{"title":"Digital Quantitative Analysis of the Relationship Between Periodontitis and Porphyromonas gingivalis","authors":"Dong Li, Weiwen Li","doi":"10.1166/jbn.2024.3853","DOIUrl":"https://doi.org/10.1166/jbn.2024.3853","url":null,"abstract":"Porphyromonas gingivalis (P. gingivalis) constitutes an essential part of the subgingival dental plaque biofilm, serving as a significant factor in the development of periodontitis. Therefore, establishing a rapid and highly sensitive detection method for P. gingivalis\u0000 is crucial to effectively manage periodontitis and its associated complications. In this study, droplet digital PCR (ddPCR) technology was employed for the detection of P. gingivalis, with a detection limit of 101 CFU/mL, exhibiting a 10-fold higher sensitivity compared to\u0000 qPCR (with a sensitivity of 102 CFU/mL). Furthermore, no cross-reactivity was observed with four other bacterial species. In comparison to real-time quantitative PCR, ddPCR demonstrated enhanced sensitivity in detecting P. gingivalis at lower concentrations in 16 simulated\u0000 samples, indicating its applicability for rapid detection of P. gingivalis.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141235014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Gong, ZhiBing Wang, Yun Zhang, Hanling Zhang, Jian Gao, Xiaoliang Li, S. Cheng, Guoxu Ma, F. Zhao
Refractory wounds in diabetic foot heal slowly. Lycium barbarum polysaccharides has been found to have the effect of lowering blood sugar. At the same time, the role of CXCL12/CXCR4 signaling in the healing process of diabetic foot has attracted much more attention. This study aimed to explore the mechanism by which Lycium barbarum polysaccharide liposome nanoparticles improve slow healing of refractory wounds in diabetic feet through CXCL12/CXCR4 signaling axis. A rat model of diabetic foot trauma was constructed and lipid nanoparticles-Lycium barbarum polysaccharides (LNP-LBP) nanocomposite was prepared and administrated into the rats. During the administration process, wound healing conditions were observed and recorded. HE staining was performed on each group, and inflammatory factors, CXCR4, and podocyte marker protein Nephrin were observed. Compared with control group, the blood sugar levels and inflammatory factor IL-6 levels of mice in the Lycium barbarum polysaccharide liposome nanoparticles group were reduced, and the wound healing speed was significantly accelerated (P < 0.05). LNP-LBP significantly reduced the levels of CXCL12 and CXCR4 in mouse wound tissues (P < 0.05). Moreover, when LNP-LBP and CXCL12/CXCR4 signaling axis inhibitors were used in combination, the wound healing speed was further accelerated and IL-6 levels were significantly increased. LNP-LBP can reduce the blood sugar level of diabetic foot rats, reduce the inflammatory response of diabetic foot wounds and swelling of wound podocytes, promote cell autophagy to speed up metabolism, thereby promoting refractory wounds healing in diabetic foot. The effect is related to inhibiting the expression of CXCL12/CXCR4 signaling.
{"title":"Application of Lycium Barbarum Polysaccharide Liposome Nanoparticles to Improve the Slow Healing of Refractory Wounds in Diabetic Foot","authors":"F. Gong, ZhiBing Wang, Yun Zhang, Hanling Zhang, Jian Gao, Xiaoliang Li, S. Cheng, Guoxu Ma, F. Zhao","doi":"10.1166/jbn.2024.3843","DOIUrl":"https://doi.org/10.1166/jbn.2024.3843","url":null,"abstract":"Refractory wounds in diabetic foot heal slowly. Lycium barbarum polysaccharides has been found to have the effect of lowering blood sugar. At the same time, the role of CXCL12/CXCR4 signaling in the healing process of diabetic foot has attracted much more attention. This study aimed\u0000 to explore the mechanism by which Lycium barbarum polysaccharide liposome nanoparticles improve slow healing of refractory wounds in diabetic feet through CXCL12/CXCR4 signaling axis. A rat model of diabetic foot trauma was constructed and lipid nanoparticles-Lycium barbarum polysaccharides\u0000 (LNP-LBP) nanocomposite was prepared and administrated into the rats. During the administration process, wound healing conditions were observed and recorded. HE staining was performed on each group, and inflammatory factors, CXCR4, and podocyte marker protein Nephrin were observed. Compared\u0000 with control group, the blood sugar levels and inflammatory factor IL-6 levels of mice in the Lycium barbarum polysaccharide liposome nanoparticles group were reduced, and the wound healing speed was significantly accelerated (P < 0.05). LNP-LBP significantly reduced the levels of\u0000 CXCL12 and CXCR4 in mouse wound tissues (P < 0.05). Moreover, when LNP-LBP and CXCL12/CXCR4 signaling axis inhibitors were used in combination, the wound healing speed was further accelerated and IL-6 levels were significantly increased. LNP-LBP can reduce the blood sugar level of\u0000 diabetic foot rats, reduce the inflammatory response of diabetic foot wounds and swelling of wound podocytes, promote cell autophagy to speed up metabolism, thereby promoting refractory wounds healing in diabetic foot. The effect is related to inhibiting the expression of CXCL12/CXCR4 signaling.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141230241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Zhu, Ailian Wang, Lan Yu, Danna Wang, Qiong Zhang, Wenqing Song
In this study, we investigated the significance of the stem cell transcription factor PLOD2 in esophageal cancer to enhance our understanding of its occurrence, development, recurrence, metastasis, and potential treatment. We conducted the following experiments: (1) Immunohistochemistry revealed elevated PLOD2 protein levels in esophageal carcinoma compared to adjacent tissues, with higher levels associated with advanced disease stages and lower differentiation. (2) Immunofluorescence demonstrated increased PLOD2 expression in esophageal cancer cell lines TE-1 and Eca- 109, suggesting a connection to cell differentiation. (3) We successfully transfected fluorescently labeled PLOD2 siRNA into cells, achieving a transfection rate of 67.57% and silencing efficiency exceeding 80%. (4) Following transfection, we observed a decreased proliferation rate in PLOD2 siRNA-treated cells, indicating that PLOD2 reduction can slow esophageal cancer growth. These findings emphasize that PLOD2 plays a crucial role in esophageal cancer pathogenesis, particularly in low cell differentiation maintenance. It could serve as a promising target for inducing differentiation in esophageal cancer and evaluating its malignancy. The high PLOD2 expression in esophageal cancer cell lines suggests the presence of tumor stem cells. Moreover, reducing PLOD2 through RNAi technology slows cell proliferation, suggesting that inhibiting PLOD2 may offer a potential therapeutic approach for esophageal cancer treatment.
{"title":"Study on Correlation and Mechanism of Procollagen Lysine 2 Oxoglutarate 5 Dioxygenase on Stem Cell Characteristics and Vasculogenic Mimicry Formation in Esophageal Squamous Cell Carcinoma","authors":"B. Zhu, Ailian Wang, Lan Yu, Danna Wang, Qiong Zhang, Wenqing Song","doi":"10.1166/jbn.2024.3866","DOIUrl":"https://doi.org/10.1166/jbn.2024.3866","url":null,"abstract":"In this study, we investigated the significance of the stem cell transcription factor PLOD2 in esophageal cancer to enhance our understanding of its occurrence, development, recurrence, metastasis, and potential treatment. We conducted the following experiments: (1) Immunohistochemistry\u0000 revealed elevated PLOD2 protein levels in esophageal carcinoma compared to adjacent tissues, with higher levels associated with advanced disease stages and lower differentiation. (2) Immunofluorescence demonstrated increased PLOD2 expression in esophageal cancer cell lines TE-1 and Eca- 109,\u0000 suggesting a connection to cell differentiation. (3) We successfully transfected fluorescently labeled PLOD2 siRNA into cells, achieving a transfection rate of 67.57% and silencing efficiency exceeding 80%. (4) Following transfection, we observed a decreased proliferation rate in PLOD2 siRNA-treated\u0000 cells, indicating that PLOD2 reduction can slow esophageal cancer growth. These findings emphasize that PLOD2 plays a crucial role in esophageal cancer pathogenesis, particularly in low cell differentiation maintenance. It could serve as a promising target for inducing differentiation in esophageal\u0000 cancer and evaluating its malignancy. The high PLOD2 expression in esophageal cancer cell lines suggests the presence of tumor stem cells. Moreover, reducing PLOD2 through RNAi technology slows cell proliferation, suggesting that inhibiting PLOD2 may offer a potential therapeutic approach\u0000 for esophageal cancer treatment.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141232428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study determines the efficacy of microRNA (miR)-124-loaded liposome nanoparticles on pancreatic cancer (PC). Herein, pancreatic cells were co-cultured with miR-124-loaded nanoparticles, pure liposome nanoparticles (empty vector group) or cultured alone (control group). The cells were administered with BECN1 inhibitor, and negative controls. The expression of autophagy-related factors (BECN1, P62, LC3) was determined by Western blot and cancer cell migration capacity was assessed by Transwell assay. The relation of miR-124 with BECN1 was assessed by bioinformatics analysis and dual-luciferase reporter gene assay. Compared with control group and the empty vector group, treatment with miR-124-loaded nanoparticles resulted in reduced number of migrated cells, scratch rate, and decreased expression of BECN1, P62, and LC3 (P < 0.05) without difference between control group and empty vector group (P > 0.05). Additional administration of BECN1 inhibitor further decreased migration and invasion of PC cells and obtained lower level of BECN1, P62, and LC3 protein, which was significantly lower than control group and miR-124+BECN1 NC group (P < 0.05). miR-124+BECN1 NC group exhibited lower expressions of BECN1, P62, and LC3 than control group (P < 0.05). Mechanistically, miR-124 targeted BECN1 to influence biological behaviors of PC cells. There is a target relationship between miR-124 and BECN1 in PC. miR-124-loaded nanoparticles incorporated with BECN1 inhibitor restrained autophagy through down-regulation of BECN1, P62, and LC3 and suppressed PC cell invasion and migration. These findings provide a novel insight into targeted therapy for PC.
{"title":"Application of MicroRNA-124-Loaded Liposome Nanoparticles for Suppressing Pancreatic Cancer Cell Progression and Restraining Autophagy Through Targeting BECN1","authors":"Weizhong Yang, Lu Xu, Xiaohong Qin","doi":"10.1166/jbn.2024.3856","DOIUrl":"https://doi.org/10.1166/jbn.2024.3856","url":null,"abstract":"This study determines the efficacy of microRNA (miR)-124-loaded liposome nanoparticles on pancreatic cancer (PC). Herein, pancreatic cells were co-cultured with miR-124-loaded nanoparticles, pure liposome nanoparticles (empty vector group) or cultured alone (control group). The cells\u0000 were administered with BECN1 inhibitor, and negative controls. The expression of autophagy-related factors (BECN1, P62, LC3) was determined by Western blot and cancer cell migration capacity was assessed by Transwell assay. The relation of miR-124 with BECN1 was assessed by bioinformatics\u0000 analysis and dual-luciferase reporter gene assay. Compared with control group and the empty vector group, treatment with miR-124-loaded nanoparticles resulted in reduced number of migrated cells, scratch rate, and decreased expression of BECN1, P62, and LC3 (P < 0.05) without difference\u0000 between control group and empty vector group (P > 0.05). Additional administration of BECN1 inhibitor further decreased migration and invasion of PC cells and obtained lower level of BECN1, P62, and LC3 protein, which was significantly lower than control group and miR-124+BECN1 NC\u0000 group (P < 0.05). miR-124+BECN1 NC group exhibited lower expressions of BECN1, P62, and LC3 than control group (P < 0.05). Mechanistically, miR-124 targeted BECN1 to influence biological behaviors of PC cells. There is a target relationship between miR-124 and BECN1 in\u0000 PC. miR-124-loaded nanoparticles incorporated with BECN1 inhibitor restrained autophagy through down-regulation of BECN1, P62, and LC3 and suppressed PC cell invasion and migration. These findings provide a novel insight into targeted therapy for PC.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141233498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chronic wounds in diabetic patients have become a common complication. However, current wound management tends to yield unsatisfactory outcomes and may give rise to complications. Therefore, we prepare a nano-structured lipid carrier by incorporating a 20(S)-Protopanaxadiol (PPD)-loaded lipid nanoparticles (PPD-N) into a silicone elastomer (PPD-NS) for treating chronic diabetic wounds and assess its therapeutic efficacy in promoting diabetic wound healing and vascular regeneration in a mouse model. Our findings revealed that the prepared PPD-N showed uniform distribution with spherical morphology and an average diameter of approximately 130 nm. Moreover, PPD-NS exhibited a wound closure rate of 92.1% after 48 hours of treatment and demonstrated enhanced vascular regeneration. Therefore, it can be concluded that the prepared PPD-NS can effectively be used in diabetic wound repair and vascular regeneration. This study provides a promising potential therapeutic option for diabetic wounds.
{"title":"Application of 20(S)-Protopanaxadiol-Loaded Nanostructured Lipid Carriers for Diabetic Wound Healing and Vascular Regeneration","authors":"Shizheng Li, Yanwei Lv, Xiaoying Liu, Yuan Yuan","doi":"10.1166/jbn.2024.3879","DOIUrl":"https://doi.org/10.1166/jbn.2024.3879","url":null,"abstract":"Chronic wounds in diabetic patients have become a common complication. However, current wound management tends to yield unsatisfactory outcomes and may give rise to complications. Therefore, we prepare a nano-structured lipid carrier by incorporating a 20(S)-Protopanaxadiol (PPD)-loaded\u0000 lipid nanoparticles (PPD-N) into a silicone elastomer (PPD-NS) for treating chronic diabetic wounds and assess its therapeutic efficacy in promoting diabetic wound healing and vascular regeneration in a mouse model. Our findings revealed that the prepared PPD-N showed uniform distribution\u0000 with spherical morphology and an average diameter of approximately 130 nm. Moreover, PPD-NS exhibited a wound closure rate of 92.1% after 48 hours of treatment and demonstrated enhanced vascular regeneration. Therefore, it can be concluded that the prepared PPD-NS can effectively be used in\u0000 diabetic wound repair and vascular regeneration. This study provides a promising potential therapeutic option for diabetic wounds.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141229132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TNF-α expression is related to myocardial function damage and recovery in patients with acute myocardial infarction, but its mechanism is not clear. 50 SD mice, 10 in each group, were in this study divided into TNF-α group, Notch1/eIF3a agonist group, model group, positive control group, and control group. The cardiac function score, myocardial infarction volume, myocardial cell apoptosis index, TNF-α expression, and Notch1/eIF3a pathway factor expression were observed. The size of polydopamine nanoparticles carrying pterostilbene was about 15.5 nm, and cardiac function score, myocardial infarction volume, myocardial cell apoptosis index, and myocardial cell apoptosis number in the model group and Notch1/eIF3a agonist group were higher than model group and Notch1/eIF3a agonist group (P < 0.05). Compared with model group, the Notch1/eIF3a agonist group, TNF-α group, and positive control group showed no differences (P > 0.05). The model group and Notch1/eIF3a agonist group had highest inflammatory response and lowest oxidative stress, which were significantly different from other groups (P < 0.05). The expression of TNF-α in Notch1/eIF3a agonist group, model group, and positive control group all decreased, which was significantly different from other groups (P < 0.05). The expressions of p-IL-6 and p-eIF3a in model group, Notch1/eIF3a agonist group and positive control group were all highest (P < 0.05). Carrying Pterostilbene-loaded polydopamine nanoparticles (PPNs) therefore inhibits apoptosis of cardiomyocytes, Notch1/eIF3a signaling pathway and inflammatory response and oxidative stress of myocardial system, and protects cardiomyocytes of model mice.
{"title":"Pterostilbene-Loaded Polydopamine Nanoparticles Down-Regulate Tumor Necrosis Factor-α and Improve Myocardial Function in Mice with Acute Myocardial Infarction","authors":"Min Zhai, Feng Bai","doi":"10.1166/jbn.2024.3852","DOIUrl":"https://doi.org/10.1166/jbn.2024.3852","url":null,"abstract":"TNF-α expression is related to myocardial function damage and recovery in patients with acute myocardial infarction, but its mechanism is not clear. 50 SD mice, 10 in each group, were in this study divided into TNF-α group, Notch1/eIF3a agonist group, model\u0000 group, positive control group, and control group. The cardiac function score, myocardial infarction volume, myocardial cell apoptosis index, TNF-α expression, and Notch1/eIF3a pathway factor expression were observed. The size of polydopamine nanoparticles carrying pterostilbene\u0000 was about 15.5 nm, and cardiac function score, myocardial infarction volume, myocardial cell apoptosis index, and myocardial cell apoptosis number in the model group and Notch1/eIF3a agonist group were higher than model group and Notch1/eIF3a agonist group (P < 0.05). Compared with\u0000 model group, the Notch1/eIF3a agonist group, TNF-α group, and positive control group showed no differences (P > 0.05). The model group and Notch1/eIF3a agonist group had highest inflammatory response and lowest oxidative stress, which were significantly different from\u0000 other groups (P < 0.05). The expression of TNF-α in Notch1/eIF3a agonist group, model group, and positive control group all decreased, which was significantly different from other groups (P < 0.05). The expressions of p-IL-6 and p-eIF3a in model group, Notch1/eIF3a\u0000 agonist group and positive control group were all highest (P < 0.05). Carrying Pterostilbene-loaded polydopamine nanoparticles (PPNs) therefore inhibits apoptosis of cardiomyocytes, Notch1/eIF3a signaling pathway and inflammatory response and oxidative stress of myocardial system,\u0000 and protects cardiomyocytes of model mice.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141229894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhaoli Lu, Peng Zhang, Jun Li, Yi Zhou, Bang Wang, Xinyan Lu
This paper aimed to observe the effect of adjuvant chemotherapy with adriamycin loaded nano-gel on osteosarcoma. 120 patients with osteosarcoma who visited the hospital from January 2018 to February 2022 were selected as control group and nano group by the red blue ball method. The control group received conventional doxorubicin combined with ifosfamide chemotherapy. The nano group was treated with doxorubicin loaded nano-gel as adjuvant chemotherapy before surgery. Both groups received limb salvage surgery. Serum alkaline phosphatase (ALP), lactate dehydrogenase (LDH), vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (bFGF) were compared between two groups before, after, and 1 day after chemotherapy. Follow up records were kept for 12 months to obtain adverse reactions to chemotherapy, postoperative clinical efficacy, and progression free survival time (PFS). No significant difference existed in ALP, LDH, VEGF, and bFGF levels between two groups of patients before chemotherapy (P > 0.05). For the overall comparison, inter group, time, and interaction effects had statistical significance (P < 0.05). For the comparison of two groups, the average ALP, LDH, VEGF, and bFGF in the nano group were lower after chemotherapy. The incidence of grade III–IV adverse reactions in the nano group was 10% lower than the controlling group. The CR+PR rate in the nano group was higher. The preoperative application of adriamycin loaded nano-gel as adjuvant chemotherapy can make the patients’ prognosis with osteosarcoma improved. It can enhance the chemotherapy effect, control the degree of adverse reactions of chemotherapy, and improve the clinical efficiency, thus extending the progression free survival period of patients with osteosarcoma.
{"title":"Doxorubicin Loaded Nano-Gel Preoperative Application Effect as Adjuvant Chemotherapy on Osteosarcoma","authors":"Zhaoli Lu, Peng Zhang, Jun Li, Yi Zhou, Bang Wang, Xinyan Lu","doi":"10.1166/jbn.2024.3868","DOIUrl":"https://doi.org/10.1166/jbn.2024.3868","url":null,"abstract":"This paper aimed to observe the effect of adjuvant chemotherapy with adriamycin loaded nano-gel on osteosarcoma. 120 patients with osteosarcoma who visited the hospital from January 2018 to February 2022 were selected as control group and nano group by the red blue ball method. The\u0000 control group received conventional doxorubicin combined with ifosfamide chemotherapy. The nano group was treated with doxorubicin loaded nano-gel as adjuvant chemotherapy before surgery. Both groups received limb salvage surgery. Serum alkaline phosphatase (ALP), lactate dehydrogenase (LDH),\u0000 vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (bFGF) were compared between two groups before, after, and 1 day after chemotherapy. Follow up records were kept for 12 months to obtain adverse reactions to chemotherapy, postoperative clinical efficacy, and progression\u0000 free survival time (PFS). No significant difference existed in ALP, LDH, VEGF, and bFGF levels between two groups of patients before chemotherapy (P > 0.05). For the overall comparison, inter group, time, and interaction effects had statistical significance (P < 0.05).\u0000 For the comparison of two groups, the average ALP, LDH, VEGF, and bFGF in the nano group were lower after chemotherapy. The incidence of grade III–IV adverse reactions in the nano group was 10% lower than the controlling group. The CR+PR rate in the nano group was higher. The preoperative\u0000 application of adriamycin loaded nano-gel as adjuvant chemotherapy can make the patients’ prognosis with osteosarcoma improved. It can enhance the chemotherapy effect, control the degree of adverse reactions of chemotherapy, and improve the clinical efficiency, thus extending the progression\u0000 free survival period of patients with osteosarcoma.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141230907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim at probe into the mechanisms of paclitaxel inhibition in thyroid cancer. Cell viability was detected via CCK8 assay, KTC-1 proliferation, migration, invasion were detected via colony formation, wound healing as well as transwell assay. Flow cytometry measure the cell cycle and apoptosis, protein expression was detected with Western blot. Iron ions, GSH and MDA were detected by corresponding assay kits, respectively. ROS levels was detected with a fluorescence probe. KTC-1 cells viability decreased significantly when treated with 500 nM paclitaxel, and the proliferation, migration as well as invasion abilities were also significantly weakened. Moreover, paclitaxel induced KTC-1 cell mitosis arrest in G2/M phase to inhibited cell mitosis, and significantly increased the apoptosis. We also found paclitaxel treatment activated AMPK/mTOR signaling pathway, and iron ion, MDA as well as ROS level were significantly increased, while GSH level and expression of GPX4 protein was notably decreased in paclitaxel-treated KTC-1 cells. Our research shows that paclitaxel significantly inhibits the viability, proliferation, migration as well as invasion of KTC-1 cells via activating the AMPK/mTOR signaling pathway, and increase oxidative stress in KTC-1 cells, inducing ferroptosis in KTC-1 cells, providing new support for paclitaxel in the treatment of thyroid cancer.
{"title":"Paclitaxel Inhibits Thyroid Cancer by Regulating AMPK/mTOR and Promoting Ferroptosis","authors":"Xiaobo Li, Bo Gui, Yafeng Yu, Fang-zhou Liu","doi":"10.1166/jbn.2024.3864","DOIUrl":"https://doi.org/10.1166/jbn.2024.3864","url":null,"abstract":"Aim at probe into the mechanisms of paclitaxel inhibition in thyroid cancer. Cell viability was detected via CCK8 assay, KTC-1 proliferation, migration, invasion were detected via colony formation, wound healing as well as transwell assay. Flow cytometry measure the cell cycle and apoptosis,\u0000 protein expression was detected with Western blot. Iron ions, GSH and MDA were detected by corresponding assay kits, respectively. ROS levels was detected with a fluorescence probe. KTC-1 cells viability decreased significantly when treated with 500 nM paclitaxel, and the proliferation, migration\u0000 as well as invasion abilities were also significantly weakened. Moreover, paclitaxel induced KTC-1 cell mitosis arrest in G2/M phase to inhibited cell mitosis, and significantly increased the apoptosis. We also found paclitaxel treatment activated AMPK/mTOR signaling pathway, and iron ion,\u0000 MDA as well as ROS level were significantly increased, while GSH level and expression of GPX4 protein was notably decreased in paclitaxel-treated KTC-1 cells. Our research shows that paclitaxel significantly inhibits the viability, proliferation, migration as well as invasion of KTC-1 cells\u0000 via activating the AMPK/mTOR signaling pathway, and increase oxidative stress in KTC-1 cells, inducing ferroptosis in KTC-1 cells, providing new support for paclitaxel in the treatment of thyroid cancer.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141233080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, we investigated the role of long non-coding RNA NEAT1 in hepatocellular carcinoma (HCC) and its impact on liver cancer cell behavior, particularly their cancer stem cell (CSC)-like properties. We observed elevated NEAT1 levels in HCC cell lines and tissues, and this upregulation was associated with adverse clinical characteristics and poor prognosis in HCC patients. Through in vitro experiments, we found that silencing NEAT1 in HCC cells led to decreased cell proliferation, migration, invasion, and inhibited epithelial-mesenchymal transition (EMT) in Huh7 cells. Additionally, the unique surface markers of CSCs were downregulated upon NEAT1 knockdown. Further investigation revealed that NEAT1 regulates the expression of GP73 by directly binding to miR-128-3p, functioning as a competitive ceRNA to suppress miR-128-3p expression. Rescue experiments showed that inhibiting miR-128-3p expression reversed the effects of NEAT1 knockdown on HCC cell proliferation,migration, and invasion. In summary, our findings demonstrate that lncRNA NEAT1 plays a pivotal role in promoting HCC progression and enhancing CSC properties by upregulating GP7 through competitive binding to miR-128-3p. This insight into the underlying mechanism may have promising implications for the treatment of HCC.
{"title":"LncRNA NEAT1 Promotes the Cancer Stem Cell-Like Properties of HCC by miR-128-3p/GP73 Axis","authors":"Ye Yuan, Xujing Zhang, Zhisu Liu","doi":"10.1166/jbn.2024.3870","DOIUrl":"https://doi.org/10.1166/jbn.2024.3870","url":null,"abstract":"In this study, we investigated the role of long non-coding RNA NEAT1 in hepatocellular carcinoma (HCC) and its impact on liver cancer cell behavior, particularly their cancer stem cell (CSC)-like properties. We observed elevated NEAT1 levels in HCC cell lines and tissues, and this upregulation\u0000 was associated with adverse clinical characteristics and poor prognosis in HCC patients. Through in vitro experiments, we found that silencing NEAT1 in HCC cells led to decreased cell proliferation, migration, invasion, and inhibited epithelial-mesenchymal transition (EMT) in Huh7 cells.\u0000 Additionally, the unique surface markers of CSCs were downregulated upon NEAT1 knockdown. Further investigation revealed that NEAT1 regulates the expression of GP73 by directly binding to miR-128-3p, functioning as a competitive ceRNA to suppress miR-128-3p expression. Rescue experiments showed\u0000 that inhibiting miR-128-3p expression reversed the effects of NEAT1 knockdown on HCC cell proliferation,migration, and invasion. In summary, our findings demonstrate that lncRNA NEAT1 plays a pivotal role in promoting HCC progression and enhancing CSC properties by upregulating GP7 through\u0000 competitive binding to miR-128-3p. This insight into the underlying mechanism may have promising implications for the treatment of HCC.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141229334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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