Antioxidant and Antineoplastic Activities of Hibiscus sabdariffa Linn. Petal Extracts against Oral Squamous Cell Carcinoma Cell Line.

IF 1.4 4区医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Oral health & preventive dentistry Pub Date : 2024-02-20 DOI:10.3290/j.ohpd.b4997059

Hadeel Mazin Akram, Azhar M Haleem, Rasha Salah

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Abstract

Purpose: To assess the antioxidant and antineoplastic effects of Hibiscus sabdariffa Linn. on oral squamous cell carcinoma cells.

Materials and methods: Human squamous cell carcinoma HSCC cells were tested for cytotoxicity by a methanol extract of Hibiscus sabdariffa (MEHSP). After 24, 48, and 72 h, the MTT assay and Trypan blue exclusion test were used to determine cell survival and death. 2, 2-diphenyl-1-picrylhydrazyl (DPPH), DNA Protection Assay (DPA), and ferric reducing antioxidant power assay (FRAPA) measured the antioxidant activity of MEHSP.

Results: The antioxidant activity (%) ranged from 47.92-82.24 in the DPPH test, 11.61-73.65 in the DPA, and 4.97-52.09 in the FRAPA. The HSCC in-vitro cytotoxicity assay showed dose- and time-dependent cell viability. MEHSP at 5 μg/ml inhibited viable cells, while increasing MEHSP doses decreased cell viability. The Trypan blue exclusion test showed that MEHSP significantly reduced cell viability at 24, 48, and 72 h.

Conclusion: Hibiscus sabdariffa contains antioxidant and HSCC-cytotoxic properties.

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木槿花瓣提取物对口腔鳞状细胞癌细胞株的抗氧化和抗肿瘤活性花瓣提取物对口腔鳞状细胞癌细胞系的抗氧化和抗肿瘤活性。

目的：评估木槿对口腔鳞状细胞癌细胞的抗氧化和抗肿瘤作用。材料与方法：用木槿甲醇提取物（MEHSP）检测人鳞状细胞癌 HSCC 细胞的细胞毒性。24、48和72小时后，用MTT试验和胰蓝排除试验来确定细胞的存活和死亡。2、2-二苯基-1-苦基肼（DPPH）、DNA 保护试验（DPA）和铁还原抗氧化力试验（FRAPA）测定了 MEHSP 的抗氧化活性：结果：DPPH 试验的抗氧化活性（%）为 47.92-82.24，DPA 为 11.61-73.65，FRAPA 为 4.97-52.09。HSCC 体外细胞毒性试验表明，细胞活力与剂量和时间有关。5 μg/ml 的 MEHSP 可抑制有活力的细胞，而 MEHSP 剂量的增加会降低细胞活力。胰蓝排除试验表明，MEHSP能显著降低细胞在24、48和72小时内的存活率：结论：木槿花具有抗氧化和抗HSCC毒性的特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral health & preventive dentistry DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Clinicians, general practitioners, teachers, researchers, and public health administrators will find this journal an indispensable source of essential, timely information about scientific progress in the fields of oral health and the prevention of caries, periodontal diseases, oral mucosal diseases, and dental trauma. Central topics, including oral hygiene, oral epidemiology, oral health promotion, and public health issues, are covered in peer-reviewed articles such as clinical and basic science research reports; reviews; invited focus articles, commentaries, and guest editorials; and symposium, workshop, and conference proceedings.