Does a therapeutical dose of ivermectin impairs testicular homeostasis of rats via excessive apoptosis?

IF 0.5 4区农林科学 Q4 VETERINARY SCIENCES Veterinaria italiana Pub Date : 2024-02-20 DOI:10.12834/VetIt.2692.19722.2

Flora Cordeiro, Flaviane L Ceglio, Nathalia A Galvão, Leoni V Bonamin, Eduardo F Bondan, Thiago Berti Kirsten, Maria M Bernardi

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Abstract

Ivermectin is a medication used to treat parasite infestations in humans and in veterinary medicine. Previously we showed that therapeutical doses of ivermectin impaired spermatogenesis and spermiogenesis in adult rats. The present study was proposed to understand the pathophysiological mechanism that triggered these impairments induced by ivermectin. It was a particular objective to study if ivermectin induced excessive apoptosis. Adult rats were treated with a therapeutical dose of ivermectin (subcutaneously). Their testis was evaluated for the expression of caspase-3 (a marker of apoptosis), using immunohistochemistry techniques. Results revealed that ivermectin treatment increased the expression of caspase-3 (labeled seminiferous tubules and strongly labeled tubules), as well as increased the number of tubules that presented labeled cells in the tubular lumen, compared to the data of the control group. In conclusion, a therapeutical dose of ivermectin induced expressive apoptosis in cells of the seminiferous tubules of rats, affecting the testicular natural homeostasis process, which resulted in the spermatogenesis and spermiogenesis impairments previously reported.

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治疗剂量的伊维菌素会通过过度凋亡损害大鼠睾丸的稳态吗？

伊维菌素是一种用于治疗人类和兽医寄生虫感染的药物。此前我们曾发现，治疗剂量的伊维菌素会损害成年大鼠的精子发生和精子形成。本研究旨在了解伊维菌素引发这些损伤的病理生理机制。研究伊维菌素是否会诱导过度的细胞凋亡是本研究的一个特别目标。成年大鼠接受治疗剂量的伊维菌素治疗（皮下注射）。使用免疫组化技术对大鼠睾丸中 Caspase-3（一种细胞凋亡标志物）的表达进行了评估。结果显示，与对照组的数据相比，伊维菌素治疗增加了 caspase-3 的表达（标记的曲细精管和强标记的曲细精管），并增加了管腔中出现标记细胞的曲细精管数量。总之，治疗剂量的伊维菌素会诱导大鼠曲细精管细胞发生表达性凋亡，影响睾丸的自然平衡过程，从而导致之前报道的精子发生和精子生成障碍。

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来源期刊

Veterinaria italiana VETERINARY SCIENCES-

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The journal was created as the Croce Azzurra in 1950. A quarterly peer-reviewed journal devoted to veterinary public health and other aspects of veterinary science and medicine, Veterinaria Italiana is published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise) in Teramo, Italy. The goal of the journal is to provide an international platform for veterinary public health information from Italy and other countries, particularly those in Eastern Europe and Africa, Asia and South America. Veterinarians and veterinary public health specialists are encouraged to share their knowledge and experience on this platform.