Development and validation of cryopreserved or freeze-dried decellularized human dermis for transplantation.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-06-01 Epub Date: 2024-02-21 DOI:10.1007/s10561-024-10131-6

Giulia Montagner, Antonia Barbazza, Manvi Pant, Andrea Tancredi Lugas, Gianpaolo Serino, Cristina Bignardi, Mara Terzini, Andrea Vantini, Jacopo Stefanelli, Diletta Trojan

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Abstract

For decades, dermal tissue grafts have been used in various regenerative, reconstructive, and augmentative procedures across the body. To eliminate antigenicity and immunogenic response while still preserving the individual components and collective structural integrity of the extracellular matrix (ECM), dermis can be decellularized. Acellular dermal matrix (ADM) products like such are produced to accurately serve diverse clinical purposes. The aim of the present study is to evaluate the efficacy of a novel decellularization protocol of the human dermis, which eliminates residual human genetic material without compromising the biomechanical integrity and collagenous content of the tissue. Moreover, a freeze-drying protocol was validated. The results showed that though our decellularization protocol, human dermis can be decellularized obtaining a biocompatible matrix. The procedure is completely realized in GMP aseptic condition, avoiding tissue terminal sterilization.

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开发和验证用于移植的低温保存或冻干脱细胞人真皮。

几十年来，真皮组织移植物一直被用于全身各种再生、重建和增生手术中。为了消除抗原性和免疫原反应，同时保留细胞外基质（ECM）的单个成分和整体结构完整性，可以对真皮进行脱细胞处理。这种细胞外基质（ADM）产品的生产可以准确地满足不同的临床目的。本研究的目的是评估一种新型人体真皮脱细胞方案的功效，该方案可消除残留的人类遗传物质，同时不损害组织的生物力学完整性和胶原蛋白含量。此外，还对冷冻干燥方案进行了验证。结果表明，通过我们的脱细胞方案，人体真皮可以脱细胞，获得生物相容性基质。该过程完全在 GMP 无菌条件下实现，避免了组织末端消毒。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.