Cell and Tissue Banking最新文献

The orthopaedic surgeries were greatly aided by bone grafting with the use of nanomaterials which provide new strategies for bone regeneration, despite the significant drawbacks of traditional treatments. Hydroxyapatite is one of the bioactive ceramics that has gained substantial research attention due to its biocompatibility, bioactivity and osteointegration ability for the manufacturing of nano bone grafts. The organized complex and porous structures of the human bone tissue is a nanocomposite which consists of both organic and inorganic matrix including hydroxyapatite naturally. Conventional hydroxyapatite was known to provide good adhesion and proliferation of host cells but very low mechanical strength. Hence biomaterial made of hydroxyapatite with various polymers and cross linking agents were used to enhance the mechanical strength of the material. Out of 293 articles obtained from the literature search, only 90 articles met the inclusion criteria about bone regeneration using nano hydroxyapatite materials. The present review addresses the potential capping agents with plant extracts for the synthesis of hydroxyapatite nanomaterials with multi-functional applications include drug delivery for targeting the desired therapeutic effect for bone regeneration with osteoprotective ability and tumour therapy.

Allograft valves offer significant advantages in valve replacement; however, their limited availability from cadaveric or brain-dead donors presents a considerable challenge. This study aims to share our experience in harvesting and preserving Allograft valves from heart transplant recipients, addressing this shortage and streamlining the process. Allograft valves were harvested from patients who underwent heart transplants at our center from October 2007 to October 2022. After sampling of the allograft for quality control, allograft valves were cryopreserved and thawed before implantation. Allograft valves were used in 60 patients who had pulmonary valve replacement (PVR) in or after repaired Tetralogy of Fallot (TOF). Patient data were collected and statistically analyzed. The age of donors was 39.0 ± 186.0 years while the median age of recipients was 5.1 years (interquartile range, 1.5-12.1). After a median follow-up of 2.6 years (interquartile range, 1.0-6.9), the freedom from all-cause mortality was 94.0%, 89.5%, and 89.5% at 5, 10, and 15 years, respectively. Adverse clinical outcomes, occurred in 24 patients (40.0%), while freedom is 64.9%, 53.4%, and 31.7% for 5, 10, and 15 years. Allograft valves bicuspidized (odds ratio, 75.085; 95% confidence interval, 10.100-558.202, P < 0.001) and early postoperative regurgitation (odds ratio, 9.946; 95% confidence interval, 1.034-95.706, P = 0.047) were considered independent risk factors for adverse clinical outcomes. Our study presents our approach to harvesting and preserving allograft valves from heart transplant recipients, demonstrating favorable short to mid-term outcomes when utilized in allograft valves for patients with or after repaired TOF. We recommend vigilant monitoring of early postoperative regurgitation, as it may signal a poor prognosis, and we strongly advise against bicuspidized the implanted allograft valves.

Purpose: This study aimed to develop a bovine ex vivoex vivo model to simulate human donor corneal storage conditions and assess the feasibility of human eye surgeries in bovine eye bulbs.

Methods: Calf eye bulbs (n = 19) were obtained from local slaughterhouses. Ten corneas were excised, with five maintained in hypothermic storage (2-8 °C): corneal quality parameters, including endothelial cell (EC) density (ECD), EC mortality and morphology, corneal transparency, and central corneal thickness, were monitored up to 14 days of preservation. Five corneas were assigned to non-vital imaging techniques to investigate cell and nuclei morphology. Human donor corneas (n = 5) in hypothermic storage were assessed as a control group. Nine bovine eye bulbs were used to mimic human eye surgeries, including capsulorhexis and open-sky vitrectomy with inner limiting membrane (ILM) removal, to evaluate their feasibility in the bovine model.

Results: Calf corneal endothelia exhibited a regular mosaic of hexagonal-shaped cells with oval-shaped nuclei and occurrence of binucleated cells. Calf ECD and EC morphology remained stable during the storage, although EC mortality and CCT increased, and corneal transparency and VECD decreased over time. ECD, EC mortality, and CCT were significantly higher in calf than in human corneas, while corneal transparency was lower. ECD change and EC morphology were statistically comparable between the two species. In the whole eye bulb, the narrow horizontal pupil reduced posterior chamber visualization, necessitating an open-sky approach for vitrectomy and ILM removal. Capsulorhexis was performed following complete iridectomy.

Conclusions: The presented bovine model offers a reliable alternative to human donor tissues for preliminary studies on corneal preservation and ophthalmic surgical procedures.

Injectable platelet-rich fibrin (I-PRF) is primarily found in liquid form and plays a role in accelerating vascularization and aiding wound healing. To carry out a critical analysis of the therapeutic potential of injectable platelet-rich fibrin (I-PRF) in the postoperative management of these surgeries. The systematic review protocol for this study included start and end dates, research question, databases, PICO structure, search strategy, inclusion/exclusion criteria, types of studies, measures of effect, screening methods, data extraction/analysis and risk of bias assessment tools. Registered with PROSPERO (CRD42024537367), it guaranteed transparency and quality. Searches were carried out on the PubMed, Scopus, Embase, Wiley Online Library and Web of Science databases. Results: The systematic search in the databases identified 532 studies. After removing duplicates with Rayyan© software, 480 documents remained, of which 15 met the inclusion criteria. After evaluation following the review protocol, 5 studies were considered highly relevant and included in the systematic review. The results indicated not only uncomplicated healing and adequate osseointegration, but also a significant improvement in comfort parameters and post-operative recovery. It was observed that the application of I-PRF accelerated the healing process, reduced pain and edema, and improved soft and hard tissue regeneration.

Antibiotics released locally through a carrier is a commonly used technique to prevent infection in orthopaedic procedures. Antibiotic-impregnated bone chips are an interesting carrier in bone reconstructive surgery. Cefazolin is a potentially interesting antibiotic given its proven efficiency in preventing surgical site infection when administered systemically. Preliminary in vitro studies with fresh frozen or processed bone chips impregnated with cefazolin solution showed rapid complete release within a few hours, questioning its potential for local infection prophylaxis. On the other hand, commercially available bone chips impregnated after purification using supercritical CO₂ have been shown to be an efficient carrier for the antibiotics vancomycin or tobramycin. With this in vitro study we wanted to investigate whether this specific type of processing protocol would improve the release pattern of cefazolin. In addition we investigated the impact of the timing of impregnation during the different steps of the processing protocol on the release of cefazolin.

A biocompatible and readily available wound dressing for emergencies has been shown to be more cost-effective, while also reducing the risk of immune system-mediated reactions. In this project, we investigated the use of a fresh blood-derived matrix as a wound dressing, based on a 3D drug-loaded Plasma-rich Fibrin (PRF) scaffold, to support the transplantation of autologous stem cells for regenerating skin tissues lost due to burns. PRF scaffold was prepared from venous blood, and adipose tissue-derived stem cells (ADSCs) were isolated from the visceral fat tissue of rats. Following in vitro analysis, PRF gel and ADSCs were transplanted onto second-degree deep burn wounds on the backs of rats. Histopathological analysis and wound size measurements were conducted on days 5, 10, 15, and 21. The findings revealed that PRF gel, as a cyto-compatible scaffold with the potential for antibacterial drug release (sustained for up to 3 days, with up to 89.7% release), significantly enhanced the healing process in the treatment group. On day 15, a reduced wound size, mature skin cells, and well-organized, thicker collagen fibers were observed in the histopathology of the PRF-treated groups, which scored an average of (2.83  ±  0.04) out of 3 for overall histopathological parameters. The greatest wound contraction was seen in the scaffold-treated groups (5.32  ±   0.61 mm²), compared with the control group (7.96 ±  0.82 mm²) (p < 0.05). PRF scaffold and ADSCs have the potential to serve as an effective biological wound dressing for burn wounds, accelerating the healing process and offering an alternative to traditional skin grafting.

Recurrent nerve entrapment syndrome is a well-known complication in peripheral nerve surgery that often leads to multiple reoperations and increases the risk of unfavorable outcomes due to scarring. In our outpatient clinic, we found two patients with recurrent nerve entrapment syndrome with significant symptoms such as complete sensory loss and chronic pain who were willing to undergo re-exploration of their entrapped nerves and cover them with a human acellular dermal matrix (ADM) as a protective shield against recurrence. Both patients had complete recovery of the nerve entrapment syndrome with satisfactory clinical results. The use of a human ADM appears to be a promising tool for recurrent nerve entrapment surgery without adding morbidity to the procedure.

In the quest for an ideal wound healing material, human amniotic membrane (AM), tilapia skin collagen (TSC), and Centella asiatica (CA) have been studied separately for their healing potential. In this study, we formulated AM, TSC, and CA gel and studied their competency and wound healing efficacy in vivo. Gel was formulated using AM, TSC, CA, Carbopol 934, acrylic acid, glycerine, and triethanolamine and physicochemical properties e.g.,pH, water absorption, swelling variation, and nuclear magnetic resonance (NMR) spectroscopy were determined. Biological properties were determined by skin irritation study, brine shrimp lethality, andantibacterial activity. Wound healing potential was determined by applying gel to second-degree burnsin vivoby observing wound contracture, epithelialization period, and histological features. The gel was non-lethal to brine shrimp and had anti-bacterial activity and showed no edema or erythema after 7 days of topical application. After 21 days of treatment, the AM + TSC + CA group significantly (P < 0.001) accelerated wound contraction (95.75 ± 0.44%)whereasthenegative control had the lowest healing rate (40.32 ± 2.11%). Wound contraction rates of AM + TSC and TSC + CA groups were (68.12 ± 1.46%) and (82.52 ± 1.74%) respectively.Epithelialization period for AM + TSC + CA was only 22.7days whereas AM, TSC, CA, AM + TSC, AM + CA, TSC + CA, positive control, and negative control needed 29.3, 30.7, 31.3, 27.3, 26, 26.6, 25.3 and 36.6 days respectively. Histological analysis showed better healing potential for AM + TSC + CA regarding epidermal regeneration, blood vessel formation, and collagen deposition. The gel was biocompatible and in vivostudies with Wistar rats exhibited better wound healing capabilities than individual components of the gel alone.

Globally there is a shortage of available donor corneas with only 1 cornea available for every 70 needed. A large limitation to corneal transplant surgery is access to quality donor tissue due to inadequate eye donation services and infrastructure in many countries, compounded by the fact that there are few available long-term storage solutions for effectively preserving spare donor corneas collected in countries with a surplus. In this study, we describe a novel technology termed low-temperature vacuum evaporation (LTVE) that can effectively dry-preserve surplus donor corneal tissue, allowing it to be stored for approximately 5 years, shipped at room temperature, and stored on hospital shelves before rehydration prior to ophthalmic surgery. The dry-preserved corneas demonstrate equivalent biological characteristics to non-dried donor tissue, with the exception that epithelial and endothelial cells are removed and keratocytes are rendered non-viable and encapsulated within the preserved extracellular matrix. Structure and composition of the dried and rehydrated corneas remained identical to that of non-dried control corneas. Matrix-bound cytokines and growth factors were not affected by the drying and rehydration of the corneas. The ability to preserve human donor corneas using LTVE will have considerable impact on global corneal supply; utilisation of preserved corneas in lamellar keratoplasties, corneal perforations, ulcers, and tectonic support, will allow non-preserved donor tissue to be reserved for where it is truly required.

Interactions between MSCs and cancer cells are complex and multifaceted and have been shown to exhibit both pro-tumor and antitumor effects. This study investigated the effects of conditioned medium (CM) and cell extract (CE) from two different ERα statuses, MCF-7 and MDA-MB-231 breast cancer cell lines, on adipose-derived mesenchymal stem cells (ASCs). Findings showed that CM and CE increased cellular metabolic activity and viability of ASCs, upregulated angiogenic factors VEGF and HIF-1α, and cytokine TGF-β expression levels. However, CM and CE treatment did not significantly affect the clonogenicity of ASCs. In addition, apoptosis-related genes caspase-3 and 9 showed differential expression patterns among the treatment groups. The findings suggest that breast cancer cell-derived factors can modulate the behavior of ASCs, highlighting their potential as a therapeutic tool in breast cancer treatment and tissue regeneration. However, it is essential to consider the potential risks associated with CM and CE treatment on ASCs, as well as the potential recruitment of ASCs by cancer tumors and the risks associated with this recruitment. Further research is needed to elucidate these potential risks and benefits.