Extracellular vesicle analysis in supramolecular 3D hydrogels: a proof-of-concept†

IF 3.5 Q2 CHEMISTRY, ANALYTICAL Sensors & diagnostics Pub Date : 2024-02-19 DOI:10.1039/D3SD00313B
Greta Bergamaschi, Roberto Frigerio, Angelo Musicò, Giulia Lodigiani, Paola Gagni, Riccardo Vago, Marina Cretich and Alessandro Gori
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Abstract

In the present work, we report a proof-of-concept application of a composite Aga-Q3 hydrogel for the gentle confinement and analysis of extracellular vesicles (EVs) on microarray analytical platforms. Thanks to their peculiar functional properties, our 3D microdroplets are suitable to phenotype EVs with specific anti-surface antigen bioprobes, including antibodies and peptides.

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超分子三维水凝胶中的细胞外囊泡分析:概念验证
在本研究中,我们报告了一种复合 Aga-Q3 水凝胶在微阵列分析平台上用于细胞外载体(EV)的温和封闭和分析的概念验证应用。由于其特殊的功能特性,我们的三维微滴适合用特异性抗表面抗原生物探针(包括抗体和肽)对 EVs 进行表型。
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