Vibrio species as next-generation chassis for accelerated synthetic biology

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology and Bioprocess Engineering Pub Date : 2024-02-19 DOI:10.1007/s12257-024-00023-7
Changhwan Hong, Yoojin Kim, Hyunjin Lee, Saebom Yun, Hyun Gyu Lim, Jina Yang, Sungho Jang
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Abstract

Synthetic biology aims to establish engineering principles for biological systems by leveraging the design-build-test-learn (DBTL) cycle. Central to the success of the DBTL cycle is the selection of a suitable chassis, which is the environment in which biological designs are tested. Every step of this cycle is strongly influenced by the properties of chassis. A successful chassis must meet various criteria, prompting ongoing research regarding new candidates. Recently, species within the Vibrio genus, notably Vibrio natriegens and related strains, have emerged as promising next-generation chassis due to their rapid growth rates, versatile substrate utilization, and biosafety level 1 classification. These properties make them highly attractive for accelerating the DBTL cycle with the potential for efficient protein and metabolite production. This review emphasizes the foundational requirements for efficient engineering in synthetic biology, including genetic parts, vectors, and genome engineering technologies tailored to Vibrio species. Practical applications, such as metabolic engineering and protein expression, have been discussed, offering a comprehensive summary of recent advances. This paper also outlines the future directions and suggestions for fully unlocking the potential of Vibrio species as next-generation chassis.

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弧菌作为加速合成生物学的下一代底盘
合成生物学旨在利用 "设计-构建-测试-学习"(DBTL)循环,为生物系统确立工程原则。DBTL 循环成功的关键是选择合适的底盘,即测试生物设计的环境。这一周期的每一步都受到底盘特性的强烈影响。成功的底盘必须符合各种标准,这就促使人们不断研究新的候选底盘。最近,弧菌属中的一些菌种,特别是纳氏弧菌和相关菌株,因其生长速度快、底物利用广泛、生物安全等级为一级而成为有前途的下一代底盘。这些特性使它们在加速 DBTL 循环方面极具吸引力,并有可能高效生产蛋白质和代谢物。本综述强调了合成生物学中高效工程的基本要求,包括基因部件、载体和针对弧菌物种的基因组工程技术。本文还讨论了代谢工程和蛋白质表达等实际应用,全面总结了最新进展。本文还概述了充分释放弧菌作为下一代底盘的潜力的未来方向和建议。
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来源期刊
Biotechnology and Bioprocess Engineering
Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物
CiteScore
5.00
自引率
12.50%
发文量
79
审稿时长
3 months
期刊介绍: Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
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