The application of silver nanoparticles green-formulated by Origanum majorana leaf extract as a hydrazine sensor and treatment of human breast cancer by following the P53 and STAT3 signaling pathways
Chao Qian , Xueli Dong , Shaik Althaf Hussain , Lei Wang , Guoliang Zhao
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引用次数: 0
Abstract
Silver nanoparticles, because of their large absorption surface and small size, are considered to be intelligent magnetic particles. The advancements in nanotechnology have revolutionized cancer treatment, with silver nanoparticles playing a crucial role in this field. In a recent experiment, the effects of Ag nanoparticles formulated from Origanum majorana on breast cancer cells were investigated. It was found that these nanoparticles induce apoptosis through the signal transducer and activator of transcription 3 and P53 signaling pathways. The nanoparticle characterization was conducted using FE-SEM, XRD, and UV–Vis techniques. Furthermore, the Ag nanoparticles exhibited significant antioxidant activity by preventing 50% of DPPH at 183 μg/mL. The MTT assay revealed the anti-breast carcinoma properties of silver NPs on MCF-7, T-47D, and SkBr3 cells. The outcomes demonstrated that as the nanoparticle concentration increased, the cancer cells survival percentage reduced for 3 days. The most effective anticancer effect was observed at 1000 μg/ml. MTT findings indicate that a concentration of nanoparticles at IC50 = 97, 186, and 180 μg/ml effectively targets 50% of MCF-7, T-47D, and SkBr3 breast carcinoma cells. The presence of silver NPs induces cell apoptosis, which is with the Bax markers regulation and the pro-apoptotic cleaved caspase-8 upregulation, while the anti-apoptotic Bcl-2 marker is downregulated. Besides, silver NPs inhibit the formation of colonies. Molecular pathway analysis of breast cells treated with silver NPs reveals an increase in p53 expression, while the total and phosphorylated STAT3 expression is inhibited, suggesting that p53 and STAT3 have a notable role in the remedial efficacies of silver NPs on breast carcinoma cells. Additionally, the Ag nanoparticles exhibit high sensitivity in the hydrazine electrochemical detection, a potentially carcinogenic substance, with a detection limit of 0.25 μM. The developed sensor, utilizing Ag nanoparticles, shows great promise for the environmental monitoring of hydrazine due to its excellent catalytic performance and simple preparation process. Based on clinical research, recent silver nanoparticles have emerged as a viable option for breast cancer treatment.
期刊介绍:
In 1985, the Journal of Science was founded as a platform for publishing national and international research papers across various disciplines, including natural sciences, technology, social sciences, and humanities. Over the years, the journal has experienced remarkable growth in terms of quality, size, and scope. Today, it encompasses a diverse range of publications dedicated to academic research.
Considering the rapid expansion of materials science, we are pleased to introduce the Journal of Science: Advanced Materials and Devices. This new addition to our journal series offers researchers an exciting opportunity to publish their work on all aspects of materials science and technology within the esteemed Journal of Science.
With this development, we aim to revolutionize the way research in materials science is expressed and organized, further strengthening our commitment to promoting outstanding research across various scientific and technological fields.