Efficient crop straws biotreatment using the fungus Cerrena Unicolor GC.u01.

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY AMB Express Pub Date : 2024-02-24 DOI:10.1186/s13568-024-01668-6
Wang Ying, Cai Chunjing, Lu Junhua, Li Xuan, Wang Zhaojiang, Chu Jie
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Abstract

Lignin is main composition of agricultural biomass which can be decomposed through enzymatic hydrolysis by fungi. However, there are still needs to identify more efficient and effective fungal stain for biomass valorization. In this study, lignin degrading fungi from birch forest were screened for sustainable degradation of waste agricultural straws. The most effective strain was identified as Cerrena unicolor GC.u01 using 18 S rDNA gene-sequencing technology. Three different crop straws (corn stalk, rice and wheat straws) were used for the biotreatment studies. The activities of lignin degrading enzymes, laccase (Lac), cellulase and xylanase, secreted by C. unicolor were also determined. Scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR) and thermal gravimetric analyzer (TGA) were further used to monitor the effects of the biotreatment process. The results showed that C. unicolor degraded 34.3% rice straw lignin, a percentage which was higher than other isolated strains after 15 d straw liquid fermentation. The highest Lac activity (8.396 U•mL- 1) was observed with corn stalk on the 7 d. Cellulase and xylanase activities, in the same biomass, were higher than those of wheat and rice straws after 15 d. Furthermore, SEM, FTIR and TGA analyses showed that C. unicolor pretreatment process had significant effects on corn stalk, rice and wheat straws' structures. The newly isolated stain of C. unicolor demonstrated high lignin degradation potential that can provide effective, ecofriendly means of valorizing biomass to industrial useable raw-material.

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利用真菌 Cerrena Unicolor GC.u01 对农作物秸秆进行高效生物处理。
木质素是农业生物质的主要成分,可以通过真菌的酶水解作用进行分解。然而,在生物质的价值化方面,仍然需要找到更高效、更有效的真菌染色剂。本研究筛选了桦树林中的木质素降解真菌,用于废弃农作物秸秆的可持续降解。利用 18 S rDNA 基因测序技术,确定了最有效的菌株为 Cerrena unicolor GC.u01。生物处理研究使用了三种不同的农作物秸秆(玉米秸秆、水稻秸秆和小麦秸秆)。此外,还测定了 C. unicolor 分泌的木质素降解酶、漆酶(Lac)、纤维素酶和木聚糖酶的活性。扫描电子显微镜(SEM)、傅立叶变换红外光谱(FTIR)和热重分析仪(TGA)被进一步用于监测生物处理过程的效果。结果表明,经过 15 天的秸秆液体发酵后,C. unicolor 降解了 34.3% 的稻草木质素,这一比例高于其他分离菌株。此外,扫描电镜、傅立叶变换红外光谱和热重分析表明,单色杆菌预处理过程对玉米秸秆、水稻和小麦秸秆的结构有显著影响。新分离出的 C. unicolor 染色体具有很高的木质素降解潜力,可为生物质转化为工业可用原料提供有效、生态友好的方法。
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来源期刊
AMB Express
AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
2.70%
发文量
141
审稿时长
13 weeks
期刊介绍: AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
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