Letizia Rasica, Erin Calaine Inglis, Raffaele Mazzolari, Danilo Iannetta, Juan M Murias
{"title":"Methodological considerations on near-infrared spectroscopy derived muscle oxidative capacity.","authors":"Letizia Rasica, Erin Calaine Inglis, Raffaele Mazzolari, Danilo Iannetta, Juan M Murias","doi":"10.1007/s00421-024-05421-6","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Different strategies for near-infrared spectroscopy (NIRS)-derived muscle oxidative capacity assessment have been reported. This study compared and evaluated (I) approaches for averaging trials; (II) NIRS signals and blood volume correction equations; (III) the assessment of vastus lateralis (VL) and tibialis anterior (TA) muscles in two fitness levels groups.</p><p><strong>Methods: </strong>Thirty-six participants [18 chronically trained (CT: 14 males, 4 females) and 18 untrained (UT: 10 males, 8 females)] participated in this study. Two trials of twenty transient arterial occlusions were performed for NIRS-derived muscle oxidative capacity assessment. Muscle oxygen consumption ( <math><mover><mi>V</mi> <mo>˙</mo></mover> </math> O<sub>2</sub>m) was estimated from deoxygenated hemoglobin (HHb), corrected for blood volume changes following Ryan (HHbR) and Beever (HHbB) equations, and from oxygen saturation (StO<sub>2</sub>) in VL and TA.</p><p><strong>Results: </strong>Superimposing or averaging <math><mover><mi>V</mi> <mo>˙</mo></mover> </math> O<sub>2</sub>m or averaging the rate constants (k) from the two trials resulted in equivalent k values [two one-sided tests (TOST) procedure with 5% equivalence margin-P < 0.001]. Whereas HHbR (2.35 ± 0.61 min<sup>-1</sup>) and HHbB (2.34 ± 0.58 min<sup>-1</sup>) derived k were equivalent (P < 0.001), StO<sub>2</sub> derived k (2.81 ± 0.92 min<sup>-1</sup>) was greater (P < 0.001) than both. k values were greater in CT vs UT in both muscles (VL: + 0.68 min<sup>-1</sup>, P = 0.002; TA: + 0.43 min<sup>-1</sup>, P = 0.01).</p><p><strong>Conclusion: </strong>Different approaches for averaging trials lead to similar k. HHb and StO<sub>2</sub> signals provided different k, although different blood volume corrections did not impact k. Group differences in k were detected in both muscles.</p>","PeriodicalId":12005,"journal":{"name":"European Journal of Applied Physiology","volume":null,"pages":null},"PeriodicalIF":2.8000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11199286/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Applied Physiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00421-024-05421-6","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/24 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PHYSIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Purpose: Different strategies for near-infrared spectroscopy (NIRS)-derived muscle oxidative capacity assessment have been reported. This study compared and evaluated (I) approaches for averaging trials; (II) NIRS signals and blood volume correction equations; (III) the assessment of vastus lateralis (VL) and tibialis anterior (TA) muscles in two fitness levels groups.
Methods: Thirty-six participants [18 chronically trained (CT: 14 males, 4 females) and 18 untrained (UT: 10 males, 8 females)] participated in this study. Two trials of twenty transient arterial occlusions were performed for NIRS-derived muscle oxidative capacity assessment. Muscle oxygen consumption ( O2m) was estimated from deoxygenated hemoglobin (HHb), corrected for blood volume changes following Ryan (HHbR) and Beever (HHbB) equations, and from oxygen saturation (StO2) in VL and TA.
Results: Superimposing or averaging O2m or averaging the rate constants (k) from the two trials resulted in equivalent k values [two one-sided tests (TOST) procedure with 5% equivalence margin-P < 0.001]. Whereas HHbR (2.35 ± 0.61 min-1) and HHbB (2.34 ± 0.58 min-1) derived k were equivalent (P < 0.001), StO2 derived k (2.81 ± 0.92 min-1) was greater (P < 0.001) than both. k values were greater in CT vs UT in both muscles (VL: + 0.68 min-1, P = 0.002; TA: + 0.43 min-1, P = 0.01).
Conclusion: Different approaches for averaging trials lead to similar k. HHb and StO2 signals provided different k, although different blood volume corrections did not impact k. Group differences in k were detected in both muscles.
期刊介绍:
The European Journal of Applied Physiology (EJAP) aims to promote mechanistic advances in human integrative and translational physiology. Physiology is viewed broadly, having overlapping context with related disciplines such as biomechanics, biochemistry, endocrinology, ergonomics, immunology, motor control, and nutrition. EJAP welcomes studies dealing with physical exercise, training and performance. Studies addressing physiological mechanisms are preferred over descriptive studies. Papers dealing with animal models or pathophysiological conditions are not excluded from consideration, but must be clearly relevant to human physiology.