Elderberry diet enhances motor performance and reduces neuroinflammation-induced cell death in cerebellar ataxia rat models

IF 2.7 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of chemical neuroanatomy Pub Date : 2024-02-23 DOI:10.1016/j.jchemneu.2024.102399
Maryam Raoofi nejad , Elham Siasi , Mohammad Amin Abdollahifar , Abbas Aliaghaei
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Abstract

Cerebellar ataxia (CA) is a condition in which cerebellar dysfunction results in movement disorders such as dysmetria, synergy and dysdiadochokinesia. This study investigates the therapeutic effects of elderberry (EB) diet on the 3-acetylpyridine-induced (3-AP) CA rat model. First, CA rat models were generated by 3-AP administration followed by elderberry diet treatment containing 2 % EB for 8 consecutive weeks. Motor performance, electromyographic activity and gene expression were then evaluated. The number of Purkinje neurons were evaluated by stereological methods. Immunohistochemistry for the microgliosis, astrogliosis and apoptosis marker caspase-3 was also performed. In addition, the morphology of microglia and astrocytes was assessed using the Sholl analysis method. The results showed that EB diet administration in a 3-AP ataxia model improved motor coordination, locomotor activity and neuro-muscular function, prevented Purkinje neurons degeneration, increased microglia and astrocyte complexity and reduced cell soma size. Moreover, EB diet administration decreased apoptosis in cerebellum of 3-AP ataxic model. In addition, elderberry diet treatment decreased the expression of inflammatory, apoptotic and necroptotic genes and increased the expression of antioxidant-related genes. The results suggest that the EB diet attenuates 3-AP-induced neuroinflammation leading to cell death and improves motor performance. Thus, the EB diet could be used as a therapeutic procedure for CA due to its neuroprotective effects.

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接骨木果实饮食可提高小脑共济失调大鼠模型的运动能力,并减少神经炎症引起的细胞死亡。
小脑共济失调(CA)是一种由小脑功能障碍导致运动障碍的疾病,如构图障碍、协同障碍和运动障碍。本研究探讨了接骨木果(EB)饮食对 3-乙酰基吡啶诱导的 CA 大鼠模型的治疗作用。首先,给大鼠服用 3-AP 后,再用含 2% EB 的接骨木果实饮食连续治疗 8 周,从而建立 CA 大鼠模型。然后对大鼠的运动表现、肌电图活动和基因表达进行评估。通过立体学方法评估了Purkinje神经元的数量。还对小胶质细胞、星形胶质细胞和细胞凋亡标志物 caspase-3 进行了免疫组化。此外,还使用 Sholl 分析方法评估了小胶质细胞和星形胶质细胞的形态。结果表明,在 3-AP 共济失调模型中服用 EB 可改善运动协调性、运动活性和神经肌肉功能,防止浦肯野神经元变性,增加小胶质细胞和星形胶质细胞的复杂性并缩小细胞体大小。此外,接骨木果实饮食还能减少 3-AP 共济失调模型小脑的细胞凋亡。此外,接骨木果实饮食还能减少炎症、凋亡和坏死基因的表达,增加抗氧化相关基因的表达。这些结果表明,接骨木果实饮食可减轻 3-AP 诱导的导致细胞死亡的神经炎症,并改善运动能力。因此,EB饮食具有神经保护作用,可用作CA的治疗方法。
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来源期刊
Journal of chemical neuroanatomy
Journal of chemical neuroanatomy 医学-神经科学
CiteScore
4.50
自引率
3.60%
发文量
87
审稿时长
62 days
期刊介绍: The Journal of Chemical Neuroanatomy publishes scientific reports relating the functional and biochemical aspects of the nervous system with its microanatomical organization. The scope of the journal concentrates on reports which combine microanatomical, biochemical, pharmacological and behavioural approaches. Papers should offer original data correlating the morphology of the nervous system (the brain and spinal cord in particular) with its biochemistry. The Journal of Chemical Neuroanatomy is particularly interested in publishing important studies performed with up-to-date methodology utilizing sensitive chemical microassays, hybridoma technology, immunocytochemistry, in situ hybridization and receptor radioautography, to name a few examples. The Journal of Chemical Neuroanatomy is the natural vehicle for integrated studies utilizing these approaches. The articles will be selected by the editorial board and invited reviewers on the basis of their excellence and potential contribution to this field of neurosciences. Both in vivo and in vitro integrated studies in chemical neuroanatomy are appropriate subjects of interest to the journal. These studies should relate only to vertebrate species with particular emphasis on the mammalian and primate nervous systems.
期刊最新文献
Editorial Board Retraction notice to “Astrocyte response to melatonin treatment in rats under high-carbohydrate high-fat diet” [J. Chem. Neuroanat. 136 (2024) 102389] Retraction notice to “Coenzyme Q10 attenuates neurodegeneration in the cerebellum induced by chronic exposure to tramadol” [J. Chem. Neuroanat. 135 (2024) 102367] Retraction notice to “Maternal diabetes-induced alterations in the expression of brain-derived neurotrophic factor in the developing rat hippocampus” [J. Chem. Neuroanat. 114(2021) 101946] Retraction notice to “Aqueous leaf extract of Phyllanthus amarus protects against oxidative stress and misfiring of dopaminergic neurons in Paraquat-induced Parkinson's disease-like model of adult Wistar rats” [J. Chem. Neuroanat. 135 (2024) 102365]
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