Phytochemical analysis, in vitro antioxidant and antifungal activities of extracts and essential oil derived from Artemisia herba-alba Asso

IF 2.1 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY Open Chemistry Pub Date : 2024-02-23 DOI:10.1515/chem-2023-0200
Faiza EL Hajli, Mohamed Reda Kachmar, Amine Assouguem, Riaz Ullah, Ahmed Bari, Khalil Hammani, Said Chakir, Rachid Lahlali, Essaïd Ait Barka, Ghizlane Echchgadda
{"title":"Phytochemical analysis, in vitro antioxidant and antifungal activities of extracts and essential oil derived from Artemisia herba-alba Asso","authors":"Faiza EL Hajli, Mohamed Reda Kachmar, Amine Assouguem, Riaz Ullah, Ahmed Bari, Khalil Hammani, Said Chakir, Rachid Lahlali, Essaïd Ait Barka, Ghizlane Echchgadda","doi":"10.1515/chem-2023-0200","DOIUrl":null,"url":null,"abstract":"<jats:italic>Artemisia herba-alba</jats:italic> Asso is an endemic plant from North-East Morocco offering a diverse range of pharmacological, cosmetic, and agro-ecological uses. However, <jats:italic>Artemisia herba-alba</jats:italic> has not been well exploited in the field of plant protection (post-harvest diseases). This is why the main objective of the present study is to evaluate the antifungal and antioxidant activities of extracts or the essential oil of this plant. Antifungal activity was assessed against <jats:italic>Botrytis cinerea</jats:italic>, <jats:italic>Penicillium expansum,</jats:italic> and <jats:italic>Penicillium digitatum</jats:italic> using the agar dilution method for the aqueous extracts and fumigation for the essential oil. Antioxidant activity was assessed using the DPPH scavenging test and the FRAP ferric ion reduction capacity. The chemical composition of the extracts was determined by HPLC and that of the essential oil by GC-MS/MS. In addition, the polyphenol, flavonoid, and tannin content was determined using colorimetric methods. The results of this study showed that the total content of polyphenols (217.60 mg GAE/g E), flavonoids (43.59 mg QE/g E), and tannins (32.58 mg GAE/g E) was significantly higher (<jats:italic>p</jats:italic> ≤ 0.05) in the ethanolic extract than in the aqueous and hexanoic extracts. Moreover, the HPLC analysis of the aqueous extract revealed the presence of six compounds, namely, catechin, gallic acid, hydroxybenzoic acid, syringic acid, caffeic acid, and rutin. In addition, the ethanolic extract was found to contain seven phenolic compounds. On the one hand, 35 compounds were identified in the essential oil of <jats:italic>Artemisia herba-alba</jats:italic>, representing 99.7% of the total. According to this study, the dominant compounds in the essential oil of <jats:italic>Artemisia herba-alba</jats:italic> Asso are camphor (46.57%), endo-borneol (5.65%), eucalyptol (5.64%), and thymol (3.85%). Furthermore, the biological evaluation showed that the extracts and essential oil of <jats:italic>Artemisia herba-alba</jats:italic> have significant antioxidant and antibacterial activities. The findings show that <jats:italic>Artemisia herba-alba</jats:italic> is a plant that can be used as a source of antifungal chemicals to prevent putrefaction of foodstuffs and, more specifically, postharvest diseases.","PeriodicalId":19520,"journal":{"name":"Open Chemistry","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1515/chem-2023-0200","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

Abstract

Artemisia herba-alba Asso is an endemic plant from North-East Morocco offering a diverse range of pharmacological, cosmetic, and agro-ecological uses. However, Artemisia herba-alba has not been well exploited in the field of plant protection (post-harvest diseases). This is why the main objective of the present study is to evaluate the antifungal and antioxidant activities of extracts or the essential oil of this plant. Antifungal activity was assessed against Botrytis cinerea, Penicillium expansum, and Penicillium digitatum using the agar dilution method for the aqueous extracts and fumigation for the essential oil. Antioxidant activity was assessed using the DPPH scavenging test and the FRAP ferric ion reduction capacity. The chemical composition of the extracts was determined by HPLC and that of the essential oil by GC-MS/MS. In addition, the polyphenol, flavonoid, and tannin content was determined using colorimetric methods. The results of this study showed that the total content of polyphenols (217.60 mg GAE/g E), flavonoids (43.59 mg QE/g E), and tannins (32.58 mg GAE/g E) was significantly higher (p ≤ 0.05) in the ethanolic extract than in the aqueous and hexanoic extracts. Moreover, the HPLC analysis of the aqueous extract revealed the presence of six compounds, namely, catechin, gallic acid, hydroxybenzoic acid, syringic acid, caffeic acid, and rutin. In addition, the ethanolic extract was found to contain seven phenolic compounds. On the one hand, 35 compounds were identified in the essential oil of Artemisia herba-alba, representing 99.7% of the total. According to this study, the dominant compounds in the essential oil of Artemisia herba-alba Asso are camphor (46.57%), endo-borneol (5.65%), eucalyptol (5.64%), and thymol (3.85%). Furthermore, the biological evaluation showed that the extracts and essential oil of Artemisia herba-alba have significant antioxidant and antibacterial activities. The findings show that Artemisia herba-alba is a plant that can be used as a source of antifungal chemicals to prevent putrefaction of foodstuffs and, more specifically, postharvest diseases.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
蒿草提取物和精油的植物化学分析、体外抗氧化和抗真菌活性
青蒿(Artemisia herba-alba Asso)是摩洛哥东北部的一种特有植物,具有多种药理、美容和农业生态用途。然而,在植物保护领域(收获后病害),蒿草还没有得到很好的利用。因此,本研究的主要目的是评估这种植物的提取物或精油的抗真菌和抗氧化活性。水提取物采用琼脂稀释法,精油采用熏蒸法,对灰霉病菌、扩张青霉和数字青霉的抗真菌活性进行了评估。抗氧化活性采用 DPPH 清除试验和 FRAP 铁离子还原能力进行评估。提取物的化学成分采用 HPLC 法测定,精油的化学成分采用 GC-MS/MS 法测定。此外,还采用比色法测定了多酚、类黄酮和单宁的含量。研究结果表明,乙醇提取物中的多酚(217.60 毫克 GAE/g E)、类黄酮(43.59 毫克 QE/g E)和单宁(32.58 毫克 GAE/g E)的总含量(p ≤ 0.05)明显高于水提取物和己酸提取物。此外,水提取物的 HPLC 分析显示存在六种化合物,即儿茶素、没食子酸、羟基苯甲酸、丁香酸、咖啡酸和芦丁。此外,乙醇提取物还含有七种酚类化合物。一方面,在艾蒿精油中发现了 35 种化合物,占总数的 99.7%。根据这项研究,阿尔巴蒿(Artemisia herba-alba Asso)精油中的主要化合物是樟脑(46.57%)、内龙脑(5.65%)、桉叶油醇(5.64%)和百里酚(3.85%)。此外,生物评估表明,青蒿提取物和精油具有显著的抗氧化和抗菌活性。研究结果表明,阿尔巴蒿是一种可用作抗真菌化学物质来源的植物,可防止食品腐败,更具体地说,可防止收获后疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Open Chemistry
Open Chemistry CHEMISTRY, MULTIDISCIPLINARY-
CiteScore
3.80
自引率
4.30%
发文量
90
审稿时长
6 weeks
期刊介绍: Open Chemistry is a peer-reviewed, open access journal that publishes original research, reviews and short communications in the fields of chemistry in an ongoing way. The central goal is to provide a hub for researchers working across all subjects to present their discoveries, and to be a forum for the discussion of the important issues in the field. The journal is the premier source for cutting edge research in fundamental chemistry and it provides high quality peer review services for its authors across the world. Moreover, it allows for libraries everywhere to avoid subscribing to multiple local publications, and to receive instead all the necessary chemistry research from a single source available to the entire scientific community.
期刊最新文献
Preparation, characterization, and determination of the therapeutic effects of copper nanoparticles green-formulated by Pistacia atlantica in diabetes-induced cardiac dysfunction in rat Scrutinizing the effect of additive and synergistic antibiotics against carbapenem-resistant Pseudomonas aeruginosa Development and characterization of functional low-fat frozen dairy dessert enhanced with dried lemongrass powder Evaluating polyphenol and ascorbic acid contents, tannin removal ability, and physical properties during hydrolysis and convective hot-air drying of cashew apple powder Effect of transition metal chloride (ZnCl2) on low-temperature pyrolysis of high ash bituminous coal
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1