Catechol- and thiol-containing binder that aggregates granular xenografts in reconstructed bone defects by mimicking mussel wet adhesion

IF 2.5 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology and Bioprocess Engineering Pub Date : 2024-02-22 DOI:10.1007/s12257-024-00025-5

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Abstract

Xenograft bone granules are bone graft materials that are widely used to reconstruct bone tissue in clinical practice, and their clinical success requires aggregation with a biocompatible binder. While the catechol mussel bioadhesive has been used as a key universal biomedical glue moiety, the thiols in mussel adhesive proteins have recently been shown to play key roles in mussel wet adhesion. Here, pentaerythritol tris(3-mercaptopropionate)-di-catechol (PETMP-di-catechol), a catechol- and thiol-containing binder molecule that mimics mussel adhesive proteins, was used to aggregate xenograft bone granules and reconstruct bone tissue. The PETMP-di-catechol-aggregated xenograft bone granules were most resistive to compression stress when both the catechol and thiol moieties in PETMP-di-catechol were chemically oxidized with sodium periodate. The xenograft bone granules aggregated with the oxidized PETMP-di-catechol exhibited enhanced osteogenic cellular behavior in vitro and in vivo in a rat calvarial defect model, compared to the control group treated with catechol-conjugated chitosan, a catechol-containing polymer binder devoid of thiol moieties. Hence, the thiol-containing catechol-functionalized binder exhibits improved bone tissue reconstruction properties by mimicking mussel wet adhesion. In addition, our study suggests that PETMP-di-catechol is a potential biocompatible bone binder for use in clinical settings.

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含儿茶酚和硫醇的粘合剂可通过模拟贻贝湿粘附作用将颗粒状异种移植物聚集在重建的骨缺损中

摘要异种骨颗粒是临床上广泛用于重建骨组织的骨移植材料，其临床成功需要与生物相容性粘合剂聚集在一起。虽然儿茶酚贻贝生物粘合剂已被用作一种关键的通用生物医学胶水分子，但最近的研究表明，贻贝粘合蛋白中的硫醇在贻贝湿粘合中起着关键作用。在这里，季戊四醇三(3-巯基丙酸)-二邻苯二酚（PETMP-二邻苯二酚）是一种模拟贻贝粘合蛋白的含儿茶酚和硫醇的粘合剂分子，被用来聚合异种移植骨颗粒并重建骨组织。当 PETMP-二儿茶酚中的儿茶酚和硫醇分子都被高碘酸钠化学氧化时，PETMP-二儿茶酚聚合的异种骨颗粒对压缩应力的抵抗力最强。在大鼠腓骨缺损模型中，与使用儿茶酚共轭壳聚糖（一种不含硫醇分子的含儿茶酚聚合物粘合剂）处理的对照组相比，使用氧化的 PETMP 二儿茶酚聚合的异种移植骨颗粒在体外和体内均表现出更强的成骨细胞行为。因此，含硫醇的儿茶酚官能化粘合剂通过模拟贻贝的湿粘附性改善了骨组织重建性能。此外，我们的研究还表明，PETMP-二儿茶酚是一种潜在的生物相容性骨粘合剂，可用于临床。

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来源期刊

Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物

CiteScore

5.00

自引率

12.50%

发文量

审稿时长

3 months

期刊介绍： Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.