Jaseela Valiya Thodiyil, Sinisha Edathumthazhe Kuni, Pradeep Nediyaparambu Sukumaran
{"title":"A modified CTAB method for extracting high-quality genomic DNA from aquatic plants","authors":"Jaseela Valiya Thodiyil, Sinisha Edathumthazhe Kuni, Pradeep Nediyaparambu Sukumaran","doi":"10.14719/pst.2850","DOIUrl":null,"url":null,"abstract":"This study introduces a streamlined approach for extractinghigh-quality DNA fromaquatic plants using CTAB, catering to molecular studies. Seven aquatic plant species(Hygrophilaauriculata, Limnophilarepens, Crinum malabaricum, Lagenandraovata, Ludwigiaperuviana, Eichhorniacrassipes, and Ipomoea aquatica) spanning six orderswere subjected toDNA extraction. The method combinesmechanical lysis and chemical treatments to effectively disrupt cells, coupled with RNase treatment and phenol extraction to mitigate RNA and protein contamination. The optimizedCTAB protocol facilitatesthe extraction of high-quality genomic DNA, suitable for amplifying plant barcode genes such as ITS and rbcL, as well asmarkerslike RAPD and ISSR, thereby enhancing the efficiency and reliability of genomic studies in aquatic plants.","PeriodicalId":509766,"journal":{"name":"Plant Science Today","volume":"79 13","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Science Today","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14719/pst.2850","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
This study introduces a streamlined approach for extractinghigh-quality DNA fromaquatic plants using CTAB, catering to molecular studies. Seven aquatic plant species(Hygrophilaauriculata, Limnophilarepens, Crinum malabaricum, Lagenandraovata, Ludwigiaperuviana, Eichhorniacrassipes, and Ipomoea aquatica) spanning six orderswere subjected toDNA extraction. The method combinesmechanical lysis and chemical treatments to effectively disrupt cells, coupled with RNase treatment and phenol extraction to mitigate RNA and protein contamination. The optimizedCTAB protocol facilitatesthe extraction of high-quality genomic DNA, suitable for amplifying plant barcode genes such as ITS and rbcL, as well asmarkerslike RAPD and ISSR, thereby enhancing the efficiency and reliability of genomic studies in aquatic plants.
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