SCCmec transformation requires living donor cells in mixed biofilms

IF 5.9 Q1 MICROBIOLOGY Biofilm Pub Date : 2024-02-16 DOI:10.1016/j.bioflm.2024.100184

Mais Maree , Yuri Ushijima , Pedro B. Fernandes , Masato Higashide , Kazuya Morikawa

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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is an important human pathogen that has emerged through the horizontal acquisition of the staphylococcal cassette chromosome mec (SCCmec). Previously, we showed that SCCmec from heat-killed donors can be transferred via natural transformation in biofilms at frequencies of 10⁻⁸-10⁻⁷. Here, we show an improved transformation assay of SCCmec with frequencies up to 10⁻² using co-cultured biofilms with living donor cells. The Ccr-attB system played an important role in SCCmec transfer, and the deletion of ccrAB recombinase genes reduced the frequency ∼30-fold. SCCmec could be transferred from either MRSA or methicillin-resistant coagulase-negative staphylococci to some methicillin-sensitive S. aureus recipients. In addition, the transformation of other plasmid or chromosomal genes is enhanced by using living donor cells. This study emphasizes the role of natural transformation as an evolutionary ability of S. aureus and in MRSA emergence.

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SCCmec 转化需要混合生物膜中的活供体细胞

耐甲氧西林金黄色葡萄球菌（MRSA）是一种重要的人类病原体，它是通过水平获取葡萄球菌盒式染色体 mec（SCCmec）而出现的。此前，我们曾发现来自热处理供体的 SCCmec 可通过生物膜中的自然转化进行转移，转化频率为 10-8-10-7。在这里，我们展示了一种改进的 SCCmec 转化检测方法，该方法使用与活体供体细胞共培养的生物膜，转化频率可达 10-2。Ccr-attB系统在SCCmec转移中发挥了重要作用，删除ccrAB重组酶基因可使转移频率降低30倍。SCCmec可以从MRSA或耐甲氧西林凝固酶阴性葡萄球菌转移到一些对甲氧西林敏感的金黄色葡萄球菌受体上。此外，使用活的供体细胞还能增强其他质粒或染色体基因的转化。这项研究强调了自然转化作为金黄色葡萄球菌进化能力以及在 MRSA 出现过程中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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