Identification and virulence test of a new pathogen that causes verticillium striping on rapeseed in northwestern China

Q3 Agricultural and Biological Sciences Oil Crop Science Pub Date : 2024-03-01 DOI:10.1016/j.ocsci.2023.12.005
We Si , Ruisheng Wang , Mingde Wu , Long Yang , Guoqing Li , Jing Zhang
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Abstract

Five stems of rapeseed with abundant black microsclerotia were collected from Huangyuan County of Qinghai Province, China, and fungal isolates were obtained from the stems. They were identified based on morphology, molecular features and specific PCR detection. The results showed that the 10 fungal isolates belonged to Verticillium longisporum lineage A1/D3. One of the 10 isolates (HW7-1) was tested for virulence on three species of rapeseed, including B. napus Zhongshuang 9, B. rapa Qingyou 9 and B. juncea Tayou 2 by conidia inoculation of HW7-1 on roots of young seedlings. Control seedlings were inoculated with V. dahliae conidia or water alone. The seedlings of these treatments were transplanted in culture mix and incubated in a growth chamber (20 ​°C). Results suggested that the control seedlings of three cultivars appeared quite healthy, while the seedlings inoculated with HW7-1 turned yellowing leaves, seedling stunting or even death after 22 days post-inoculation. V. longisporum was re-isolated from he yellow leaves, thus fulfilling Koch's postulates. Moreover, compared to the control treatments, inoculation with HW7-1 caused flowering delay and seed yield reduction on Tayou 2 with production of microsclerotia on the stems. To our knowledge, this is the first report of V. longisporum lineage A1/D3 on rapeseed in northwestern China.

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导致中国西北地区油菜纹枯病的一种新病原体的鉴定和毒力试验
从中国青海省湟源县采集了五株带有大量黑色小圆菌丝的油菜茎,并从茎中获得了真菌分离物。根据形态学、分子特征和特异性 PCR 检测对它们进行了鉴定。结果表明,这 10 株真菌属于长孢霉 A1/D3 株系。通过在幼苗根部接种 HW7-1 分生孢子,测试了 10 个分离株中的一个(HW7-1)对 3 种油菜籽的毒力,包括油菜中双 9 号、油菜青优 9 号和油菜大优 2 号。对照组幼苗只接种大丽花病毒分生孢子或水。将这些处理的幼苗移栽到培养混合液中,并在生长室(20 °C)中培养。结果表明,三个栽培品种的对照秧苗看起来相当健康,而接种 HW7-1 的秧苗在接种后 22 天叶片变黄、秧苗发育不良甚至死亡。从黄叶中重新分离出了长孢霉,从而验证了科赫推论。此外,与对照处理相比,接种 HW7-1 会导致 Tayou 2 的开花延迟和种子减产,并在茎上产生小圆菌丝。据我们所知,这是中国西北地区油菜上长孢霉 A1/D3 株系的首次报道。
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来源期刊
Oil Crop Science
Oil Crop Science Food Science, Plant Science, Agronomy and Crop Science
CiteScore
3.40
自引率
0.00%
发文量
20
审稿时长
74 days
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