Oil Crop Science最新文献

Ameliorative effects of flaxseed-based plant milk on water restriction-induced constipation in mice 亚麻籽植物乳对小鼠限水便秘的改善作用

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-12-15 DOI: 10.1016/j.ocsci.2025.10.001

Congcong Ma, Lei Wang, Yashu Chen, Qianchun Deng, Qingde Huang, Jiqu Xu

Traditional Chinese medicine has long regarded flaxseed as a natural remedy for constipation. However, the efficacy or mechanism of flaxseed plant milk (FPM), a functional beverage derived from flaxseed, in alleviating constipation has not yet been clearly established. In this study, network pharmacology was combined with in vivo experiments to evaluate the anti-constipation activity of FPM on water restriction-induced constipation mouse model. Compared with the model group, administration of FPM (100 mg/kg and 200 mg/kg) significantly shortened the time to first black stool excretion, increased number of fecal pellets and water content of fecal pellets, and improved Small Intestinal Transit Rate (p < 0.05). Histological analysis revealed that FPM (200 mg/kg) preserved colonic epithelial integrity, reduced lamina propria damage, and restored goblet cell populations. Serum biochemical assays showed that FPM decreased vasoactive intestinal peptide (VIP) levels and increased 5-hydroxytryptamine (5-HT) concentrations. Network pharmacology identified 214 overlapping targets between flaxseed bioactive compounds and constipation-related genes, with key hub targets including TP53, CASP3, TNF, and IL6. KEGG pathway enrichment suggested that the PI3K/AKT signaling pathway may serve as a central regulatory axis. Western blot further confirmed that p-PI3K and p-AKT protein levels in the colon were reduced in constipated mice, whereas FPM administration markedly restored their expression, indicating activation of the PI3K/AKT pathway. Overall, these findings demonstrate that FPM alleviates constipation by improving stool characteristics, enhancing intestinal motility, and maintaining mucosal integrity, potentially via modulation of the PI3K/AKT pathway. This study provides new insights into the gastrointestinal benefits of flaxseed-derived functional foods and supports their development as dietary supplements for intestinal health.

传统中医一直认为亚麻籽是治疗便秘的天然药物。然而，亚麻籽植物乳（flaxseed plant milk， FPM）作为一种从亚麻籽中提取的功能性饮料，其缓解便秘的功效或机制尚未明确。本研究采用网络药理学与体内实验相结合的方法，评价FPM对限水便秘小鼠模型的抗便秘活性。与模型组比较，FPM （100 mg/kg和200 mg/kg）可显著缩短大鼠第一次黑便排出时间，增加粪球数量和粪球含水量，提高小肠转运率（p < 0.05）。组织学分析显示，FPM （200 mg/kg）可保持结肠上皮的完整性，减少固有层损伤，恢复杯状细胞群。血清生化分析显示，FPM降低了血管活性肠肽（VIP）水平，升高了5-羟色胺（5-HT）浓度。网络药理学鉴定了214个亚麻籽生物活性化合物与便秘相关基因之间的重叠靶点，其中关键的枢纽靶点包括TP53、CASP3、TNF和IL6。KEGG通路的富集表明PI3K/AKT信号通路可能是中心调控轴。Western blot进一步证实便秘小鼠结肠中p-PI3K和p-AKT蛋白水平降低，而FPM显著恢复了它们的表达，表明PI3K/AKT通路被激活。总的来说，这些发现表明，FPM通过改善大便特征、增强肠道蠕动和维持粘膜完整性来缓解便秘，可能是通过调节PI3K/AKT通路。本研究为亚麻籽衍生功能食品对胃肠道的益处提供了新的见解，并支持其作为肠道健康膳食补充剂的发展。

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引用次数: 0

Winners of the Brazilian soybean yield contest: climatic, soil, management, and economic factors 巴西大豆产量竞赛的赢家：气候、土壤、管理和经济因素

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-12-01 DOI: 10.1016/j.ocsci.2025.07.003

Julierme Zimmer Barbosa , Antonio Carlos Vargas Motta , Giovana Poggere , Stephen A. Prior

Soybean yield contests have been generating useful information regarding the cultivation of this important crop commodity. The aim of this study was to analyze environmental, management, and economic variables associated with soybean yield contest winners in Brazil. Data from eleven summer soybean harvests included grain yield, rainfall, climatic and agronomic efficiency, soil chemical and physical attributes, management strategies, and economic indicators. Two grain yield classes were evaluated: 1) high yield (4967–6839 kg ha⁻¹) and 2) very high yield (6859–8945 kg ha⁻¹). Enhanced soybean yield was due to adequate rainfall that led to high climatic efficiency (77 %) and high agronomic efficiency; average values ranged between 78 % (high yield class) and 86 % (very high yield class). Correction and maintenance of soil chemical and physical quality (surface and subsurface), alternative fertilization and microbial co-inoculation were notable. Winners in the very high yield class reported higher soil K⁺, Ca²⁺, and Mg²⁺ levels, more frequent use of dolomitic limestone, greater soil structural quality, greater use of biostimulants (via foliar spray), and higher net revenue. While high climatic efficiency was fundamental for success, intensive integration of technologies with conservation practices for better soil and crop management are necessary to maximize soybean yield and economic gains.

大豆产量竞赛提供了有关这一重要作物商品种植的有用信息。本研究的目的是分析与巴西大豆产量竞赛获胜者相关的环境、管理和经济变量。11个夏季大豆收获的数据包括粮食产量、降雨量、气候和农艺效率、土壤化学和物理属性、管理策略和经济指标。评估了两个粮食产量等级：1)高产（4967-6839 kg ha - 1）和2)非常高产（6859-8945 kg ha - 1）。大豆产量的提高是由于充足的降雨带来了高气候效率（77%）和高农艺效率；平均值在78%（高产类）和86%（极高产类）之间。土壤化学和物理质量（表层和地下）的矫正和维持、替代施肥和微生物共接种效果显著。高产作物的土壤K+、Ca2+和Mg2+含量较高，使用白云岩石灰岩的频率更高，土壤结构质量更高，使用生物刺激素（通过叶面喷雾）的频率更高，净收入更高。虽然气候效率高是成功的基础，但为了最大限度地提高大豆产量和经济收益，必须将技术与保护措施紧密结合起来，以更好地管理土壤和作物。

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引用次数: 0

Multiple adulteration detection of olive oils by Raman spectroscopy 橄榄油多重掺假的拉曼光谱检测

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-12-01 DOI: 10.1016/j.ocsci.2025.08.001

Xue Li , Du Wang , Li Yu , Fei Ma , Xuefang Wang , Liangxiao Zhang , Peiwu Li

The increasing prevalence of multiple adulteration in olive oil affects product authenticity and market integrity. Therefore, it is necessary to develop advanced methods to detect the multiple adulteration. In this study, a portable Raman spectrometer was employed to address this issue, with adulterant mixtures using a D-optimal mixture design to ensure representative samples. The results showed that principal component analysis (PCA) was unable to distinguish authentic olive oils from adulterated ones, whereas partial least squares-discriminant analysis (PLS-DA) successfully differentiated the two groups. These findings demonstrated the technical feasibility of combining Raman spectroscopy with PLS-DA to detect multiple adulteration in olive oil, offering a promising method for portable, rapid authentication to counter increasingly sophisticated fraud in the edible oil industry.

橄榄油多重掺假现象日益普遍，影响了产品的真实性和市场的完整性。因此，有必要开发先进的检测多重掺假的方法。在本研究中，采用便携式拉曼光谱仪解决这一问题，掺假混合物采用d -最优混合设计，以确保样品具有代表性。结果表明，主成分分析（PCA）无法区分正品橄榄油和掺假橄榄油，而偏最小二乘判别分析（PLS-DA）成功区分了两者。这些发现证明了将拉曼光谱与PLS-DA相结合检测橄榄油中多种掺假的技术可行性，为应对食用油行业日益复杂的欺诈行为提供了一种便携式、快速的认证方法。

引用次数: 0

Overexpression of demethylase-related gene FTO improves salt and drought tolerance in rapeseed 脱甲基酶相关基因FTO的过度表达提高了油菜的耐盐性和耐旱性

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-12-01 DOI: 10.1016/j.ocsci.2025.12.001

Yuting Zou , Rijin Zhou , Yinghai Xu , Mengyu Hao , Wenxiang Wang , Jia Liu , Wenliang Wei

Drought and salt stresses are major abiotic factors that severely affect the growth, development, and yield formation of Brassica napus. Human derived FTO gene (Fat mass and obesity-associated), is a member of the AlkB family, encoding a mRNA demethylase that is dependent on α-ketoglutarate. Although previous studies have demonstrated that exogenous overexpression of FTO gene can increase plant biomass, its impact on plant stress resistance is still unclear. In this study, we cloned the FTO gene and conducted an analysis of its biological functions for drought and salt resistance for Brassicaceae plants. By overexpressing the FTO gene in Arabidopsis thaliana, the inhibitory effect of salt and drought stress on the root length growth of transgenic lines was significantly lower than that of the control. Moreover, the overexpression of FTO markedly enhanced the tolerance of Arabidopsis to drought and salt stress. It also led to a decrease in malondialdehyde (MDA) content, an increase in proline content, and a boost in superoxide dismutase (SOD) activity. Meanwhile, when the FTO gene was heterologously expressed in B. napus, the transgenic plants were less affected by stress. In comparison to control plants, they exhibited significantly lower MDA levels and markedly higher proline content and SOD activity. Furthermore, staining results with Trypan blue and nitroblue tetrazolium (NBT) staining indicate that the FTO gene can alleviate the damage to plants under stress and inhibit the accumulation of O₂⁻. Comprehensively, the results indicate that overexpression of the FTO gene can improve the drought and salt tolerance in transgenic plants, providing valuable references for further exploring the FTO-mediated stress resistance mechanisms.

干旱和盐胁迫是严重影响甘蓝型油菜生长发育和产量形成的主要非生物因子。人类衍生的FTO基因（脂肪质量和肥胖相关）是AlkB家族的成员，编码依赖于α-酮戊二酸的mRNA去甲基化酶。虽然已有研究表明外源过表达FTO基因可以增加植物生物量，但其对植物抗逆性的影响尚不清楚。在本研究中，我们克隆了FTO基因，并对其在芸苔科植物抗旱、耐盐方面的生物学功能进行了分析。通过在拟南芥中过表达FTO基因，盐胁迫和干旱胁迫对转基因品系根长生长的抑制作用显著低于对照。此外，FTO的过表达显著增强了拟南芥对干旱和盐胁迫的耐受性。它还导致丙二醛（MDA）含量降低，脯氨酸含量增加，超氧化物歧化酶（SOD）活性增强。同时，当FTO基因在甘蓝型油菜中异种表达时，转基因植株受胁迫的影响较小。与对照植株相比，其MDA含量显著降低，脯氨酸含量和SOD活性显著提高。此外，台番蓝和硝基蓝四唑（NBT）染色结果表明，FTO基因可以减轻胁迫对植物的伤害，抑制O2−的积累。综上所述，FTO基因的过表达可以提高转基因植物的耐旱性和耐盐性，为进一步探索FTO介导的抗逆性机制提供了有价值的参考。

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引用次数: 0

Effects of sowing peanut with shells on key agronomic traits and yield 播带壳花生对主要农艺性状及产量的影响

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-12-01 DOI: 10.1016/j.ocsci.2025.04.009

Kunkun Zhao , Xufa Du , Pan Zhang, Jiakai Song, Peiyuan Du, Qikang Wang, Dongmei Yin

The peanut farming system plays a crucial role in the development of the agricultural industry. Traditionally, peanuts are sown as shelled seeds; however, systematic research on the agronomic performance and yield potential of planting with shells remains limited. In this study, we present a novel approach—peanut sowing with shells (P-S-S)—that promotes plant growth, enhances root development, and increases yield. This method also improves seed quality and elevates protein and oil content. Field trials conducted in the Huang-Huai-Hai region and northeastern China demonstrate the technique's broad adaptability, resulting in an average yield increase of 12.9%, with gains reaching up to 41% in northeastern areas, even under a 20% reduction in seeding rate. We have refined a standardized planting protocol for P-S-S based on the principle of “Two Reductions and Two Increases”: reduced input costs and post-harvest losses, along with increased production and economic benefits. This innovation contributes significantly to the advancement of peanut cultivation practices in China.

花生种植制度在农业产业的发展中起着至关重要的作用。传统上，花生是作为带壳种子播种的；然而，目前对贝壳种植的农艺性能和产量潜力的系统研究还很有限。在这项研究中，我们提出了一种新的方法-花生带壳播种(P-S-S) -促进植物生长，促进根系发育，提高产量。这种方法还可以改善种子质量，提高蛋白质和油脂含量。在黄淮海地区和中国东北地区进行的田间试验表明，该技术具有广泛的适应性，平均产量提高了12.9%，在播种率降低20%的情况下，东北地区的产量提高了41%。我们根据“两减两增”原则，制定了P-S-S标准化种植方案，即降低投入成本和收获后损失，同时提高产量和经济效益。这一创新为中国花生种植实践的进步做出了重大贡献。

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引用次数: 0

The impact of advanced rapeseed pretreatment technology on minor compounds in rapeseed oil: A review 先进的菜籽预处理技术对菜籽油中微量化合物的影响

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-12-01 DOI: 10.1016/j.ocsci.2025.11.001

Dan Wang , Jinghan Miao , Hua Zhang , Hong Chen , Fang Wei

Rapeseed is the primary domestic source of edible vegetable oil in China. Its oil is naturally rich in phytosterols, polyphenols, tocopherols, carotenoids and other lipid-soluble companions, which are highly beneficial to human health. Since the content and bioaccessibility of these health-promoting companions are mostly locked during the first post-harvest operation, the pretreatment technology has become the decisive lever for simultaneously maximizing oil yield and nutritional value. This review critically synthesizes recent findings on how rapeseed pretreatment modulates its signature lipid companions, contrasts conventional roasting with emerging pulsed-electric field, microwave and steam-explosion technologies, and evaluates their respective impacts on extraction efficiency, structural integrity and functional performance on phytosterols, polyphenols, tocopherols and carotenoids. It particularly compares and analyzes the extraction efficiency, cost-effectiveness, and environmental impact for these emerging technologies, and discusses the prospects for their integrated applications. Furthermore, the review concludes with the current research trends in pretreatment technology and offers insights into future research directions, aiming to provide scientific guidance for the high-quality processing of rapeseed and the production of rapeseed oil with high nutritional value.

菜籽是中国国内食用植物油的主要来源。其油天然富含植物甾醇、多酚、生育酚、类胡萝卜素等脂溶性伴侣，对人体健康非常有益。由于这些有益健康的伴生物的含量和生物可及性大多在采收后的第一次操作中被锁定，因此预处理技术成为同时实现油脂产量和营养价值最大化的决定性杠杆。本文综述了油菜籽预处理如何调节其特征脂质伙伴的最新研究成果，对比了传统烘焙与新兴的脉冲电场、微波和蒸汽爆炸技术，并评估了它们各自对植物甾醇、多酚、生育酚和类胡萝卜素的提取效率、结构完整性和功能性能的影响。特别对这些新兴技术的萃取效率、成本效益和环境影响进行了比较和分析，并讨论了它们综合应用的前景。综述了目前预处理技术的研究趋势，并对未来的研究方向进行了展望，旨在为油菜籽的高品质加工和高营养价值油菜籽油的生产提供科学指导。

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引用次数: 0

Genome-wide association study (GWAS) identifies genetic loci and candidate genes for variation in protein and oil content in Sesamum indicum L. 全基因组关联研究（GWAS）鉴定了芝麻蛋白和油脂含量变异的遗传位点和候选基因。

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-12-01 DOI: 10.1016/j.ocsci.2025.08.002

Habtamu Kefale , Rong Zhou , Muez Berhe , Ahmed A. Abbas , Chris O. Ojiewo , Huan Li , Ting Zhou , Jun You , Linhai Wang

Sesame (Sesamum indicum L.) is an ancient diploid oilseed crop valued for its high oil content, quality protein, unsaturated fatty acids, and antioxidants. The emerging research focuses on the utilization of vegetable proteins in the development of novel products and the formulation of functional foods. Developing sesame genotypes with higher protein and oil content is a current focus for improving sesame quality traits, as preferred by the sesame seed oil industry and consumers. Despite previous genome-wide association studies (GWAS) on various agronomic and biochemical traits in sesame, the genetic basis of protein content and oil content remains limited. Therefore, this study was designed to identify candidate genes and quantitative trait loci (QTLs) regulating the target traits using 401 sesame accessions grown in three environments (2018WH, 2018LQ, and 2022WH). The protein and oil content in the sesame population varied significantly. The protein content ranged from 15.06% to 26.45%, while the oil content was between 41.68% and 60.15%. Furthermore, the white seeds had a higher oil content, while the black seeds had a higher protein content, indicating an association between seed colour and oil and protein content. GWAS results revealed that 632 and 269 QTLs were selected as significant genetic regions for protein and oil content, respectively. Of these significant QTLs, we identified 10 and 22 stable QTLs for protein and oil content, respectively. A total of 17 candidate genes related to protein and oil content were identified within 32 stable quantitative trait loci (QTLs). Interestingly, two variant sites, SNP13:10093594 and InDel13:10935497, related to protein content, were identified in the gene sequences of SINPZ1300586 and SINPZ1300694. Four SNPs, SNP1:22608987, SNP4:13355820, SNP8:21686488, and SNP11:142842, for oil content were found correspond to the genes SINPZ0102106, SINPZ0401222, SINPZ0801731, and SINPZ1100015, respectively. The findings provide light on the genetic basis of protein and oil characteristics. These genes and QTLs can aid the molecular breeding and genetic engineering of innovative sesame germplasm, with improved potential for protein and oil content, as well as desired quality attributes.

芝麻（Sesamum indicum L.）是一种古老的二倍体油籽作物，因其高含油量、优质蛋白质、不饱和脂肪酸和抗氧化剂而受到重视。新兴的研究重点是利用植物蛋白开发新产品和功能食品的配方。开发蛋白质和油脂含量较高的芝麻基因型是目前改善芝麻品质性状的重点，受到芝麻油行业和消费者的青睐。尽管已有关于芝麻各种农艺和生化性状的全基因组关联研究，但蛋白质含量和油脂含量的遗传基础仍然有限。因此，本研究旨在利用在2018WH、2018LQ和2022WH三种环境下生长的401份芝麻材料，鉴定调控目标性状的候选基因和数量性状位点（qtl）。不同芝麻群体的蛋白质和油脂含量差异显著。蛋白质含量为15.06% ~ 26.45%，含油量为41.68% ~ 60.15%。此外，白色种子的油含量较高，而黑色种子的蛋白质含量较高，表明种子颜色与油和蛋白质含量之间存在关联。GWAS结果显示，分别有632个和269个qtl作为蛋白质含量和油脂含量的显著遗传区域。在这些重要的qtl中，我们分别鉴定出10个和22个稳定的蛋白质和油含量qtl。在32个稳定数量性状位点（qtl）中共鉴定出17个与蛋白质和油脂含量相关的候选基因。有趣的是，在SINPZ1300586和SINPZ1300694基因序列中发现了两个与蛋白质含量相关的变异位点SNP13:10093594和InDel13:10935497。结果发现，与含油量有关的4个snp分别为：SNP1:22608987、SNP4:13355820、SNP8:21686488和SNP11:142842，分别与SINPZ0102106、SINPZ0401222、SINPZ0801731和SINPZ1100015基因对应。这一发现为蛋白质和油脂特性的遗传基础提供了线索。这些基因和qtl可用于创新芝麻种质资源的分子育种和遗传工程，具有提高蛋白质和油脂含量的潜力，以及所需的品质属性。

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引用次数: 0

Development of novel genotypes of peanut with resistance to stem rot, large pod and seed 花生抗茎腐病、大荚果和种子新基因型的培育

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-12-01 DOI: 10.1016/j.ocsci.2025.07.001

Wanduo Song, Dongyang Yu, Yanping Kang, Qianqian Wang, Yong Lei, Zhihui Wang, Dongxin Huai, Xin Wang, Boshou Liao, Yuning Chen, Liying Yan

Stem rot caused by Agroathelia rolfsii (syn. Sclerotium rolfsii) is one of the major biotic constraints to peanut production in many countries, particularly under high temperature and humidity conditions. Developing disease-resistant cultivars represents a more sustainable strategy. To generate peanut germplasm integrating stem rot resistance and elite yield traits, this study utilized a recombinant inbred line (RIL) population, consisting of 242 lines, derived from a cross between Zhonghua212 (medium-seeded, resistant parent), and Zhonghua21 (large-seeded, susceptible parent). A multi-environment evaluation was conducted for disease resistance profiling in the fields with artificial inoculation, and for yield-related traits assessment. The results indicated that twenty-two RIL lines exhibited consistent moderate resistance across all tested locations. Fifty RILs consistently expressed large pod (100-pod weight >180 g) and large seed (100-seed weight >80 g) phenotypes in two environments. Three elite RIL lines (BJF66, BJF119 and BJF137) combined moderate resistance with superior pod/seed traits, providing valuable genetic sources for breeding programs targeting both disease resistance and productivity. This study established a foundational germplasm pool for advancing stem rot resistant, high-yielding peanut varieties, aligning with sustainable agricultural practices to mitigate A. rolfsii threats.

在许多国家，特别是在高温高湿条件下，由罗尔夫氏核霉（Agroathelia rolfsii）引起的茎腐病是制约花生生产的主要生物之一。培育抗病品种是一种更可持续的策略。为了获得集抗茎腐和优良产量性状于一体的花生种质资源，本研究利用中华212（中种子，抗性亲本）和中华21（大种子，易感亲本）杂交的242个重组自交系（RIL）群体。采用多环境评价方法，对人工接种大田的抗病性进行了分析，并对产量性状进行了评价。结果表明，22个RIL系在所有测试地点表现出一致的中等抗性。50个ril在两种环境下一致表达大荚果（100粒重180g）和大种子（100粒重80g）表型。三个RIL精英品系（BJF66、BJF119和BJF137）结合了中等抗性和优异的荚果/种子性状，为抗病和高产育种计划提供了宝贵的遗传资源。本研究为培育抗茎腐病高产花生品种建立了基础种质资源库，并与可持续农业实践相一致，以减轻罗氏僵杆菌的威胁。

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引用次数: 0

Effect of ARC-BBBE microbial inoculant on main phytohormones in peanut root from seedling to podding stage ARC-BBBE微生物接种剂对花生苗期至结荚期根系主要激素的影响

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-12-01 DOI: 10.1016/j.ocsci.2025.05.004

Sichen Guan , Jin Mao , Qiaomei Qin , Na Li , Ling Cheng , Xianglong Yang , Qi Zhang , Peiwu Li

Phytohormones play a crucial role in regulating peanut growth and development. Our previous studies have demonstrated that the microbial inoculant ARC-BBBE, developed by our research group, effectively promotes peanut growth and enhances yield under both greenhouse and field conditions. Therefore, it is of significant interest to investigate how ARC-BBBE influences the levels and spatial distribution of major phytohormones in peanut roots. Greenhouse pot experiments revealed that ARC-BBBE significantly enhanced peanut growth and root system development. A systematic analysis of the effects of ARC-BBBE on key phytohormones in peanut roots across different growth stages showed that gibberellin A₃ (GA₃) content varied markedly, with predominant accumulation occurring during the early growth stage, whereas changes in indole-3-acetic acid (IAA) levels were not statistically significant. Specifically, GA₃ content in the ARC-BBBE treatment group was 1.27-fold higher than in the control group during the seedling stage. Furthermore, peanut growth parameters were significantly improved following ARC-BBBE application, particularly at the flowering stage, where plant height, above-ground biomass, root length, and root weight in the treated group were 1.24-, 1.17-, 1.13-, and 1.21-fold greater than those in the control, respectively. To elucidate the functional role of phytohormones in ARC-BBBE-mediated growth promotion, we examined the effects of exogenous GA₃ and its biosynthesis inhibitor uniconazole (S3307) on both PHNZY-23-3 rhizobial growth and peanut development. Results indicated that supplementation with 1 × 10³ mg/L GA₃ most effectively promoted peanut growth at the seedling stage, while S3307 application inhibited growth. These findings provide valuable insights into the mechanism by which ARC-BBBE modulates GA₃ dynamics to enhance peanut growth, offering a foundation for future research on plant-microbe interactions and phytohormone regulation.

植物激素在调节花生生长发育中起着至关重要的作用。前期研究表明，课题组研制的微生物接种剂ARC-BBBE在温室和田间条件下均能有效促进花生生长，提高产量。因此，研究ARC-BBBE对花生根系主要植物激素水平和空间分布的影响具有重要意义。温室盆栽试验表明，ARC-BBBE能显著促进花生生长和根系发育。通过系统分析ARC-BBBE对花生不同生育期根系关键激素的影响，发现赤霉素A3 （GA3）含量变化显著，且以生长早期积累为主，而吲哚-3-乙酸（IAA）含量变化无统计学意义。在苗期，ARC-BBBE处理组的GA3含量是对照组的1.27倍。此外，施用ARC-BBBE后，花生的生长参数显著改善，特别是在开花期，处理组的株高、地上生物量、根长和根重分别比对照高1.24倍、1.17倍、1.13倍和1.21倍。为了阐明植物激素在arc - bbbe介导的生长促进中的功能作用，我们研究了外源GA3及其生物合成抑制剂uniconazole （S3307）对PHNZY-23-3根瘤菌生长和花生发育的影响。结果表明，苗期添加1 × 103 mg/L GA3对花生生长最有效，而施用S3307对花生生长有抑制作用。这些发现为探究ARC-BBBE调控GA3促进花生生长的机制提供了有价值的见解，为未来植物-微生物相互作用和植物激素调控的研究奠定了基础。

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引用次数: 0

Effects of compound microbial agents and cellulase on the fermentation quality and nutritional characteristics of Camellia oleifera cake 复合微生物剂与纤维素酶对油茶饼发酵品质及营养特性的影响

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-12-01 DOI: 10.1016/j.ocsci.2025.07.002

Zhenxia Xu , Yi Zhang , Yi Zhong , Weijuan Su , Minghao Liu , Xia Xiang , Yangmin Gong

Camellia oleifera cake (COC), a nutrient-rich by-product of tea oil extraction, holds promise as a high-quality protein source but is limited in feed applications due to anti-nutritional factors, mainly tea saponins and crude fiber. This study employed solid-state fermentation using a compound microbial agent combined with cellulase to enhance COC's palatability and nutritional value. Single-strain fermentation identified Lactiplantibacillus plantarum as most effective in degrading tea saponins (46.0%) without reducing crude fiber. Optimal conditions were 0.1% inoculum, 48 h at 37 °C, with 20% sugar. For cellulase hydrolysis, the best parameters were: 50 U/g enzyme, 50 °C, 8 h, using 40-mesh sieved substrate. Combined fermentation began with enzymatic treatment followed by inoculation with a 1: 1: 1: 1: 1: 1 mixture of Saccharomyces cerevisiae (two strains), Lactiplantibacillus plantarum, Bacillus subtilis, Bacillus coagulans, and Lactobacillus acidophilus. The optimized conditions (0.1% inoculum, 7 days, 37 °C, 20% sugar, 50% moisture) significantly reduced anti-nutritional components and improved protein content, indicating the potential of fermented COC as a viable feed ingredient.

油茶饼（Camellia oleifera cake， COC）是一种营养丰富的茶油提取副产品，有望成为一种高质量的蛋白质来源，但由于茶皂苷和粗纤维等抗营养因子的存在，其在饲料中的应用受到限制。本研究采用复合微生物制剂与纤维素酶复合的固态发酵方法，提高COC的适口性和营养价值。单菌种发酵发现植物乳杆菌在不还原粗纤维的情况下对茶皂苷的降解效果最好（46.0%）。最佳接种量为0.1%，37℃下接种48 h，添加20%糖。纤维素酶水解的最佳工艺参数为：50 U/g酶，50℃，8 h， 40目筛底物。联合发酵从酶处理开始，然后接种1:1:1:1:1:1混合的酿酒酵母菌（两株）、植物乳杆菌、枯草芽孢杆菌、凝固芽孢杆菌和嗜酸乳杆菌。优化后的条件（接种量0.1%,7 d, 37℃，20%糖，50%水分）显著降低了抗营养成分，提高了蛋白质含量，表明发酵COC作为一种有活力的饲料原料的潜力。

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