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Ameliorative effect of walnut oil against cognitive impairment in alzheimers type dementia in rodent 核桃油对啮齿动物阿尔茨海默型痴呆认知功能障碍的改善作用
Q3 Agricultural and Biological Sciences Pub Date : 2024-11-01 DOI: 10.1016/j.ocsci.2024.09.003
Congcong Ma , Li Zhang , Lei Wang , Qingde Huang , Qianchun Deng , Fenghong Huang , Jiqu Xu
Walnut oil (WO), known for abundant polyunsaturated fatty acids and an array of bioactive substances such as tocopherols, phytosterols, squalene, melatonin, and polyphenols, which is endowed with numerous health advantages. The primary objective of this research was to ascertain the impact of WO on cognitive deficits in learning and memory impairment mice caused by scopolamine (SCOP). The Morris water maze and the step-down avoidance test were utilized to assess the memory and learning capabilities. WO notably counteracted the detrimental effects of SCOP on learning and memory in the Morris water maze, as indicated by a reduction in escape latency and swimming distance. Likewise, WO administration led to a notably reduced number of errors in training trial and an increased latency in testing trial when compared to the SCOP group in the step-down avoidance test,. Moreover, WO activated the cholinergic system of the brain by upregulating choline acetyltransferase activity and reducing acetylcholinesterase activity. These results suggest that WO has the potential to protect against memory decline in mice, offering a promising strategy for the prevention of memory-related disorders.
核桃油(WO)以丰富的多不饱和脂肪酸和一系列生物活性物质(如生育酚、植物甾醇、角鲨烯、褪黑激素和多酚)而闻名,具有众多健康优势。本研究的主要目的是确定 WO 对东莨菪碱(SCOP)导致的学习和记忆障碍小鼠认知缺陷的影响。研究采用了莫里斯水迷宫和降阶回避测试来评估小鼠的记忆和学习能力。在莫里斯水迷宫中,WO明显抵消了SCOP对学习和记忆的不利影响,这表现在逃逸潜伏期和游泳距离的缩短上。同样,在降阶回避测试中,与 SCOP 组相比,施用 WO 可显著减少训练试验中的错误次数,增加测试试验中的潜伏期。此外,WO 还能通过上调胆碱乙酰转移酶活性和降低乙酰胆碱酯酶活性来激活大脑的胆碱能系统。这些结果表明,WO 具有防止小鼠记忆力衰退的潜力,为预防记忆相关疾病提供了一种很有前景的策略。
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引用次数: 0
Rapid determination of oil content of single peanut seed by near-infrared hyperspectral imaging 利用近红外高光谱成像技术快速测定单粒花生种子的含油量
Q3 Agricultural and Biological Sciences Pub Date : 2024-09-06 DOI: 10.1016/j.ocsci.2024.05.002
Shunting Zhang , Xue Li , Du Wang , Li Yu , Fei Ma , Xuefang Wang , Mengxue Fang , Huiying Lyu , Liangxiao Zhang , Zhiyong Gong , Peiwu Li
Oil content is a crucial indicator for evaluating the quality of peanuts. A rapid and non-destructive method to determine oil content of individual peanut seed can provide robust technical support for breeding high-oil-content peanut varieties. In this study, we established a rapid determination method using near-infrared hyperspectral imaging and chemometrics to assess the oil content of single peanut seed. After selecting key wavelengths through competitive adaptive reweighted sampling (CARS), uninformative variable elimination (UVE), and random frog (RF), we constructed an oil content calibration model based on partial least squares regression for single peanut seed. Validation results demonstrated that the correlation coefficient was 0.8393 with a root mean square error of 1.7771 in the calibration set, while it was 0.7915 with a root mean square error of 2.2943 in the independent prediction set. Most samples exhibited relative errors below 5%, confirming the reliability of this model in predicting oil content of single peanut seed.
含油量是评价花生质量的一个重要指标。快速、无损地测定单粒花生种子含油量的方法可为培育高含油量花生品种提供有力的技术支持。在本研究中,我们建立了一种利用近红外高光谱成像和化学计量学评估单粒花生种子含油量的快速测定方法。通过竞争性自适应加权采样(CARS)、无信息变量剔除(UVE)和随机蛙法(RF)选择关键波长后,我们构建了基于偏最小二乘回归的单粒花生种子含油量校准模型。验证结果表明,校准集的相关系数为 0.8393,均方根误差为 1.7771;独立预测集的相关系数为 0.7915,均方根误差为 2.2943。大多数样本的相对误差低于 5%,这证实了该模型在预测单粒花生种子含油量方面的可靠性。
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引用次数: 0
Solvent-free synthesis of diacylglycerols via enzymatic glycerolysis between edible oils and glycerol catalyzed by self-made immobilized lipase PS@LXTE-1000 自制固定化脂肪酶 PS@LXTE-1000 催化食用油与甘油之间的酶促甘油分解,无溶剂合成二酰甘油
Q3 Agricultural and Biological Sciences Pub Date : 2024-09-06 DOI: 10.1016/j.ocsci.2024.05.003
Yuhao Li , Yi Zhang , Run Liu , Zhonghui Liu , Kheng-Lim Goh , Vladimir Zivkovic , Mingming Zheng
Diglycerol (DAG) is a structural lipid with the functions to lower body fat accumulation and decrease serum triglyceride level. However, the enzymatic synthesis of DAG is limited by the high-efficient and economic lipases. In this paper, the immobilized lipase PS@LXTE-1000 was self-made by immobilizing the Pseudomomas cepacian lipase on to the hydrophobic microporous resin LXTE-1000. The results indicate that LXTE-1000 was a uniform mesoporous sphere with the mean diameter of 400 ​μm, pore size of 14.6 ​nm, pore volume of 0.5 ​cm3/g and surface area of 126.0 ​m2/g, showing superior structural properties for lipase immobilization. Under the optimal reaction conditions with the molar ratio of rapeseed oil to glycerol being 1:1, adding amount of immobilized lipase being 4%, reaction at 50 ​°C, the highest DAG content of 46.7% was achieved in 3 ​h via enzymatic glycerolysis catalyzed by LXTE-1000. After 7 cycles of reuse, the self-made LXTE-1000 could still retain 78.3% of its initial catalytic ability. Besides, LXTE-1000 was observed to facilitate the DAG production via glycerolysis reaction between glycerol with other seven edible oils including corn oil, sesame oil, peony seed oil, rice bran oil, peanut oil, soybean oil and flaxseed oil. Specifically, the glycerolysis reaction with sesame oil, peony seed oil and rice bran oil even led to the DAG content of 52.1%, 53.3% and 51.2%, respectively, Hence, this paper provide a novel strategy to produce high-efficient and economic immobilized lipases, which shows great potential in the green synthesis of functional lipids such as DAG.
二甘油(DAG)是一种结构脂质,具有降低体内脂肪堆积和血清甘油三酯水平的功能。然而,酶法合成 DAG 受限于高效经济的脂肪酶。本文利用疏水性微孔树脂 LXTE-1000 自制了固定化脂肪酶 PS@LXTE-1000。结果表明,LXTE-1000 是一个均匀的介孔球体,平均直径为 400 μm,孔径为 14.6 nm,孔体积为 0.5 cm3/g,比表面积为 126.0 m2/g,显示出固定脂肪酶的优异结构特性。在菜籽油与甘油的摩尔比为 1:1、固定化脂肪酶的添加量为 4%、反应温度为 50 ℃的最佳反应条件下,LXTE-1000 催化的酶促甘油分解反应在 3 小时内达到了 46.7% 的最高 DAG 含量。自制的 LXTE-1000 在重复使用 7 次后,仍能保持 78.3% 的初始催化能力。此外,还观察到 LXTE-1000 可促进甘油与其他七种食用油(包括玉米油、芝麻油、牡丹籽油、米糠油、花生油、大豆油和亚麻籽油)发生甘油分解反应生成 DAG。因此,本文提供了一种生产高效、经济的固定化脂肪酶的新策略,在绿色合成 DAG 等功能脂类方面具有巨大潜力。
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引用次数: 0
Effects of adding flaxseed milk coproduct and okara on the quality and glycemic response of Chinese steamed bread 添加亚麻籽乳副产品和黄卡拉对中式馒头质量和血糖反应的影响
Q3 Agricultural and Biological Sciences Pub Date : 2024-09-06 DOI: 10.1016/j.ocsci.2024.06.007
Yan Tang , Yaqiong Pei , Jiahui Wang , Haichao He , Mingkai Sun , Yashu Chen , He Liu , Hu Tang , Qianchun Deng
Flaxseed milk coproduct (FMC) is a by-product of flaxseed milk. Okara is a by-product of processed soybean products. In this study, we investigated the quality of dough and Chinese steamed bread (CSB) with the addition of FMC and okara. We also examined the in vitro starch digestibility, expected glycemic index (eGI), starch crystallinity, and short-range order structure of CSB. The results showed that FMC and okara decreased the dough fluidity, formed a dense structure, and enhanced the mechanical properties of the dough. FMC and okara increased the hardness, gumminess, and chewiness of the CSB, while decreasing its cohesion and elasticity. The addition of FMC and okara improved the nutrient content of CSB and reduced the eGI from 75.86 to 51.56. FMC and okara altered the multiscale structure of starch, effectively shielding the amylase site of action, and limited the interaction between amylase and starch. This study provides a reference for the high-value utilization of oilseed processing by-products.
亚麻籽奶副产品(FMC)是亚麻籽奶的副产品。奥卡拉是大豆加工产品的副产品。在这项研究中,我们调查了添加 FMC 和黄卡拉的面团和中式馒头(CSB)的质量。我们还考察了体外淀粉消化率、预期血糖生成指数(eGI)、淀粉结晶度和中式馒头的短程有序结构。结果表明,FMC 和黄卡拉降低了面团的流动性,形成了致密结构,提高了面团的机械性能。FMC 和黄卡拉增加了 CSB 的硬度、胶质感和咀嚼感,同时降低了其内聚力和弹性。添加 FMC 和黄原胶提高了 CSB 的营养成分,使 eGI 从 75.86 降至 51.56。FMC 和黄卡拉改变了淀粉的多尺度结构,有效地屏蔽了淀粉酶的作用位点,限制了淀粉酶与淀粉之间的相互作用。这项研究为油籽加工副产品的高值化利用提供了参考。
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引用次数: 0
Use of oilseed crops biomass for heavy metal treatment in water 利用油料作物生物质处理水中的重金属
Q3 Agricultural and Biological Sciences Pub Date : 2024-07-01 DOI: 10.1016/j.ocsci.2024.07.001
Carlos Peña-Guzmán , Ángela Otálvaro-Álvarez , Tatiana Jiménez-Ariza

The treatment of heavy metals in water is of high importance worldwide, and different treatment types have been developed. The use of plant material is becoming more and more important, and oilseed crops biomass have been investigated in terms of phytoremediation and biosorption processes. This article is a review of the literature reporting the applications in 10 different plants and evaluating the removal efficiencies for 12 metals, including the findings of 81 publications. Moringa olifera and Helianthus annuus are the most studied plants, whereas Cu (21.9%), Cd (18.5%), and Pb (19.9%) are the most studied metals. As a result, it was found that more than 90% of Pb, Cu, Cd, Fe, Zn, Ni, Cr, Sr and Mn showed removals in their experiments. At the same time, the variables most related to the efficiency of metal removal are pH, temperature, and contact time. This article includes a review of the biosorption isotherms used in the different studies.

处理水中的重金属在全球范围内都具有重要意义,并已开发出不同的处理类型。植物材料的使用正变得越来越重要,油籽作物生物质在植物修复和生物吸附过程方面也得到了研究。本文是一篇文献综述,报告了 10 种不同植物的应用情况,并评估了 12 种金属的去除效率,包括 81 篇出版物的研究结果。研究最多的植物是 Moringa olifera 和 Helianthus annuus,而研究最多的金属是铜(21.9%)、镉(18.5%)和铅(19.9%)。结果发现,90%以上的铅、铜、镉、铁、锌、镍、铬、锶和锰在其实验中被去除。同时,与金属去除效率关系最大的变量是 pH 值、温度和接触时间。本文回顾了不同研究中使用的生物吸附等温线。
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引用次数: 0
Sodium nitroprusside as a signal molecule for up-regulating membrane characteristics, antioxidant defense system to improve flax productivity under water stress 硝普钠作为一种信号分子,可上调膜特性和抗氧化防御系统,提高亚麻在水分胁迫下的产量
Q3 Agricultural and Biological Sciences Pub Date : 2024-07-01 DOI: 10.1016/j.ocsci.2024.07.002
N.M. Al-Ashkar , B.A. Bakry , H.M.S. El-Bassiouny , M.M.S. Abdallah , M.S. Sadak

Water stress is a critical environmental adversity that significantly impacts the growth, development, and yield of flax plants. In this study, flax seeds were cultivated under different water irrigation requirements (WIR) (100%, 75%, and 50%) to investigate the effects of exogenously supplied nitric oxide (NO) donor sodium nitroprusside (SNP) as foliar treatments at concentrations of 0.0 ​mmol/L, 0.5 ​mmol/L, 1.0 ​mmol/L, and 2.0 ​mmol/L. Drought stress led to a significant decrease in plant growth, photosynthetic pigments, yield components such as oil and total carbohydrate percentage. It also resulted in an increase in leaf H2O2 production, lipid peroxidation levels and activities of enzymatic antioxidants including polyphenol oxidase, superoxide dismutase, and nitrate reductase enzymes. However, foliar application of SNP improved photosynthetic pigments and antioxidant defense system which mitigated the negative impact of water stress on growth and yield productivity by reducing oxidative damage caused by reactive oxygen species accumulation. The use of SNP also decreased H2O2 accumulation levels, lipid peroxidation levels, and improved membrane stability. SNP treatment at concentration of 2 ​mmol/L showed superior results compared to other concentrations with extremely significant increases observed in yield characteristics such as oil content, total carbohydrate percentages, and unsaturated fatty acids to saturated fatty acids ratio.

水胁迫是一种严重影响亚麻植物生长、发育和产量的关键环境逆境。在这项研究中,亚麻种子在不同的灌溉水要求(WIR)(100%、75% 和 50%)下进行栽培,研究外源一氧化氮(NO)供体硝普钠(SNP)作为叶面处理(浓度分别为 0.0 mmol/L、0.5 mmol/L、1.0 mmol/L 和 2.0 mmol/L)的影响。干旱胁迫导致植物生长、光合色素、产量成分(如油和总碳水化合物百分比)显著下降。干旱胁迫还导致叶片 H2O2 生成量、脂质过氧化水平和酶抗氧化剂(包括多酚氧化酶、超氧化物歧化酶和硝酸还原酶)活性增加。然而,叶面喷施 SNP 改善了光合色素和抗氧化防御系统,通过减少活性氧积累造成的氧化损伤,减轻了水分胁迫对生长和产量的负面影响。使用 SNP 还能降低 H2O2 积累水平和脂质过氧化水平,并提高膜的稳定性。与其他浓度相比,2 毫摩尔/升浓度的 SNP 处理效果更佳,在油脂含量、总碳水化合物百分比和不饱和脂肪酸与饱和脂肪酸比率等产量特征方面都有极其显著的提高。
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引用次数: 0
Screening and identification of salt tolerance soybean varieties and germplasms 筛选和鉴定大豆品种和种质的耐盐性
Q3 Agricultural and Biological Sciences Pub Date : 2024-07-01 DOI: 10.1016/j.ocsci.2024.06.005
Limiao Chen , Lihua Peng , Wenqi Ouyang , Haowen Yao , Yuxin Ye , Zhihui Shan , Dong Cao , Shuilian Chen , Zhonglu Yang , Yi Huang , Bei Han , Aihua Sha , Xinan Zhou , Haifeng Chen

Soil salinization is a globally prevalent abiotic environmental stress. The imbalance of ions caused by high concentrations of sodium chloride results in a 40% reduction in soybean yield. Soybean, as an important crop for soil quality improvement, necessitates the identification of salt-tolerant varieties and germplasms to effectively utilize and enhance saline-alkali land. In this study, we assessed the salt tolerance of 435 soybean varieties and germplasms during the seedling stage. Among them, Qihuang34, You2104, Hongzhudou, Pamanheidou, and Osage exhibited grade 1 salt tolerance rates surpassing other tested materials. Furthermore, Hongzhudou and Qihuang34 demonstrated higher salt tolerance during germination and emergence stages based on their elevated rates of emergence, salt tolerance index, chlorophyll content, and shoot fresh weights. Overall findings provide valuable resources for molecular breeding efforts aimed at developing salt-tolerant soybean varieties suitable for cultivation in saline-alkali soils.

土壤盐碱化是全球普遍存在的非生物环境压力。高浓度氯化钠造成的离子失衡导致大豆减产 40%。大豆作为改良土壤质量的重要作物,需要鉴定耐盐品种和种质,以有效利用和改良盐碱地。本研究评估了 435 个大豆品种和种质在苗期的耐盐性。其中,"岐黄34"、"优2104"、"红豆"、"帕曼黑豆 "和 "奥萨奇 "的耐盐性超过了其他测试材料,达到了 1 级。此外,红豆和岐黄34在萌芽和出苗阶段表现出更高的耐盐性,这体现在其出苗率、耐盐性指数、叶绿素含量和嫩枝鲜重的提高上。总体研究结果为分子育种工作提供了宝贵的资源,旨在开发适合在盐碱地种植的耐盐大豆品种。
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引用次数: 0
An introductory review on the common brown leafhopper (Orosius orientalis): A new soybean pest 普通褐叶蝉(Orosius orientalis)介绍性综述:一种新的大豆害虫
Q3 Agricultural and Biological Sciences Pub Date : 2024-07-01 DOI: 10.1016/j.ocsci.2024.07.003
Tianhao Pei , Xin Cui , Shusen Shi , Yu Gao

Soybean pests are one of the major factors limiting yield improvement. With the expansion of area and changes in cropping patterns, a number of new pests have been identified in the main soybean production areas of China. The common brown leafhopper, Orosius orientalis, is a new pest associated with soybean stay-green virus that has been discovered on cultivated soybean crop in the Yellow-Huai-hai region of China in recent years. The polyphagous insect has a wide feeding range and infests a variety of important grain and cash crops. This paper presents the basic information, geographical distribution, hosts, damage characteristics, plant virus transmission, occurrence patterns, and prevention and control measures O. orientalis. This review also provides insights into integrated prevention and control of the genus Orosius as an insect vector.

大豆虫害是制约产量提高的主要因素之一。随着种植面积的扩大和种植模式的改变,我国大豆主产区发现了一些新的害虫。普通褐叶蝉(Orosius orientalis)是近年来在中国黄淮海地区发现的一种与大豆留绿病毒有关的新害虫。该虫为多食性昆虫,取食范围广,为害多种重要粮食作物和经济作物。本文介绍了东方尺蠖的基本信息、地理分布、寄主、危害特征、植物病毒传播、发生规律和防控措施。这篇综述还为综合防控作为昆虫媒介的东方雨燕属提供了见解。
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引用次数: 0
Genetic variability and trait association analysis in linseed (Linum usitatissimum L.) for yield and related traits 亚麻籽(Linum usitatissimum L.)产量及相关性状的遗传变异和性状关联分析
Q3 Agricultural and Biological Sciences Pub Date : 2024-07-01 DOI: 10.1016/j.ocsci.2024.06.003
Sandeep Kumar Yadav , Neha Singh , Hemant Kumar Yadav

Diversity information mining about a crop for different attributes is an essential step for effective breeding programs. The present investigation evaluates the quantum of genetic variability and determines the relationship among the important agro-economic traits based on two years of phenotypic data of 210 accessions of linseed. The traits, capsule weight per plant, capsule per plant, husk weight per plant, and seed weight per plant exhibited comparatively higher genetic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV). In contrast, oil content and seed per capsule exhibited a lower value. The high magnitude of broad sense heritability was observed for all traits except seeds per capsule and husk weight per plant. The trait, capsules per plant, plant height, and days to 50% flowering showed high genetic advance coupled with high heritability. Hierarchical cluster analysis grouped 210 accessions into six distinct clusters. Out of 210, 144 (68.57%) accessions were grouped into three clusters (I, II, and III), in which cluster-III was the largest, containing 64 accessions followed by cluster II and cluster-I. The highest inter-cluster distance was observed between clusters-I and V (127.85), while the lowest was between clusters-II and IV (27.09). The positive correlation of capsule weight per plant with the seed weight per plant and a negative correlation with the days to 50% flowering indicates that high yielding linseed varieties with early flowering/maturity could be developed through direct and indirect selection. Further, seed yield and oil content could be enhanced together as indicated by ghe positive association among these two important traits. In this study, high yielding accessions with moderate to high oil content such as GP36, GP31, GP14, GP54, GP26, GP24, GP34, GP21, GP37 and GP27 and early flowering (less than 70 days) accessions such as GP2, GP26, GP27, CG33, CG44, CG42, CG132, and CG31 identified as potential genetic materials that could be exploited for developing early maturing varieties with high yield. In addition, information’s on various genetic parameters will help breeders to devise suitable breeding methodology for linseed genetic improvement for targeted traits.

作物不同属性的多样性信息挖掘是有效育种计划的必要步骤。本研究根据 210 个亚麻籽品种两年的表型数据,评估了遗传变异量,并确定了重要农业经济性状之间的关系。单株蒴果重、单株蒴果重、单株谷壳重和单株种子重等性状的遗传变异系数(GCV)和表型变异系数(PCV)相对较高。相比之下,含油量和每粒种子的变异系数较低。除每粒蒴果种子和每株谷壳重量外,所有性状的广义遗传力都很高。单株蒴果、株高和 50%开花天数等性状表现出较高的遗传进展和遗传率。层次聚类分析将 210 个品种分为 6 个不同的聚类。在 210 个品种中,144 个品种(68.57%)被归入三个聚类(I、II 和 III),其中聚类-III 最大,包含 64 个品种,其次是聚类 II 和聚类-I。簇间距离最大的是簇 I 和簇 V(127.85),最小的是簇 II 和簇 IV(27.09)。单株蒴果重量与单株种子重量呈正相关,而与 50%开花天数呈负相关,这表明可以通过直接和间接选育培育出早花/早熟的高产亚麻籽品种。此外,种子产量和含油量这两个重要性状之间的正相关关系也表明,种子产量和含油量可以同时提高。在这项研究中,GP36、GP31、GP14、GP54、GP26、GP24、GP34、GP21、GP37 和 GP27 等中高含油量的高产品种和 GP2、GP26、GP27、CG33、CG44、CG42、CG132 和 CG31 等早花(少于 70 天)品种被确定为潜在的遗传材料,可用于开发高产早熟品种。此外,有关各种遗传参数的信息将有助于育种者制定合适的育种方法,以针对目标性状进行亚麻籽遗传改良。
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引用次数: 0
CRISPR/Cas9-mediated knockout of E4 gene promotes maturation in soybean CRISPR/Cas9 介导的 E4 基因敲除可促进大豆成熟
Q3 Agricultural and Biological Sciences Pub Date : 2024-07-01 DOI: 10.1016/j.ocsci.2024.05.001
Shuiqing Wu , Li Chen , Mengwei Guo , Yupeng Cai , Yang Gao , Shan Yuan , Shi Sun , Yuxian Zhang , Wensheng Hou , Tianfu Han

Soybean is a broadly popular and extensively cultivated crop, however, many high-yield and high-quality varieties require specific growth conditions, restricting their widespread adoption. The appropriate light conditions and photoperiod must be attained for these varieties to thrive in new environments. In this study, we employed CRISPR/Cas9 to design two sgRNAs aimed at knocking out the maturity-related gene E4 in a major American soybean variety called ''Jack'', which belongs to maturity group MGII. E4 gene is primarily involved in the photoperiodic flowering and maturity in soybean, making it an ideal candidate for genetic manipulation. We successfully obtained 1 homozygous E4-SG1 mutant type with 1-bp insertion, and 4 homozygous E4-SG2 mutants type with 2-bp deletion, 7-bp deletion, 61-bp deletion, and 1-bp insertion, respectively. The homozygous e4 mutant plants contained early termination codons devoid of transgenic elements. Additionally, no potential off-target sites of the E4 gene were detected. A comparative analysis revealed that, unlike the wild-type, the maturity time of homozygous e4 mutants was early under both short-day and long-day conditions. These mutants offer novel germplasm resources that may be used to modify the photoperiod sensitivity and maturity of soybean, enhancing its adaptability to high-latitude regions.

大豆是一种广受欢迎和广泛种植的作物,然而,许多高产优质品种需要特定的生长条件,这限制了它们的广泛采用。要使这些品种在新环境中茁壮成长,必须获得适当的光照条件和光周期。在本研究中,我们利用 CRISPR/Cas9 设计了两个 sgRNA,旨在敲除美国大豆主要品种 "杰克"(Jack)的成熟相关基因 E4。E4 基因主要参与大豆的光周期开花和成熟,因此是遗传操作的理想候选基因。我们成功获得了 1 个 1-bp 插入的同源 E4-SG1 突变体,以及 4 个分别为 2-bp 缺失、7-bp 缺失、61-bp 缺失和 1-bp 插入的同源 E4-SG2 突变体。同源的 e4 突变体植株含有早期终止密码子,没有转基因元件。此外,没有检测到 E4 基因的潜在脱靶位点。比较分析表明,与野生型不同,同源 e4 突变体在短日照和长日照条件下的成熟时间都较早。这些突变体提供了新的种质资源,可用于改变大豆的光周期敏感性和成熟度,提高其对高纬度地区的适应性。
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引用次数: 0
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Oil Crop Science
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