Microscopy methods to visualize nuclear organization in biomechanical studies

IF 4.7 3区 工程技术 Q2 ENGINEERING, BIOMEDICAL Current Opinion in Biomedical Engineering Pub Date : 2024-02-16 DOI:10.1016/j.cobme.2024.100528
Hannah Hyun-Sook Kim , Melike Lakadamyali
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Abstract

The mechanical environment plays an important role in influencing cell identity. The nucleus's organization and mechanical state are essential regulators of cellular function. However, open questions remain about the mechanisms underlying how the physical microenvironment influences nuclear mechanics and organization to drive specific transcriptional and epigenetic shifts. Understanding how biophysical cues change cell behavior provides groundwork to improve medical technologies such as tissue engineering, stem cell therapy, and mitigation of aberrant cell behavior. Microscopy is an indispensable tool that noninvasively explores the cell's nuclear state, providing valuable measurements on features including nuclear morphology, nuclear mechanical properties, protein localization, and genomic organization. In this review, we discuss notable imaging techniques, such as super-resolution microscopy, examples of how they have recently advanced the field, and how they can further our knowledge of the interplay between nuclear mechanoregulation and cell function.

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在生物力学研究中观察核组织的显微镜方法
机械环境在影响细胞特性方面发挥着重要作用。细胞核的组织和机械状态是细胞功能的重要调节因素。然而,物理微环境如何影响细胞核的力学和组织,从而驱动特定的转录和表观遗传转变,其背后的机制仍是一个未决问题。了解生物物理线索如何改变细胞行为,为改进组织工程、干细胞治疗和缓解异常细胞行为等医疗技术奠定了基础。显微镜是一种不可或缺的工具,它能无创探查细胞核状态,对核形态、核机械特性、蛋白质定位和基因组组织等特征进行有价值的测量。在这篇综述中,我们将讨论超分辨显微镜等著名成像技术、它们如何在最近推动这一领域发展的实例,以及它们如何进一步增进我们对核机械调控与细胞功能之间相互作用的了解。
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来源期刊
Current Opinion in Biomedical Engineering
Current Opinion in Biomedical Engineering Medicine-Medicine (miscellaneous)
CiteScore
8.60
自引率
2.60%
发文量
59
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