Fabrication of vascularized tissue-engineered bone models using triaxial bioprinting

IF 3.9 3区 医学 Q2 ENGINEERING, BIOMEDICAL Journal of biomedical materials research. Part A Pub Date : 2024-02-27 DOI:10.1002/jbm.a.37694
Junbiao Zhang, Srisurang Suttapreyasri, Chidchanok Leethanakul, Bancha Samruajbenjakun
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Abstract

Bone tissue is a highly vascularized tissue. When constructing tissue-engineered bone models, both the osteogenic and angiogenic capabilities of the construct should be carefully considered. However, fabricating a vascularized tissue-engineered bone to promote vascular formation and bone generation, while simultaneously establishing nutrition channels to facilitate nutrient exchange within the constructs, remains a significant challenge. Triaxial bioprinting, which not only allows the independent encapsulation of different cell types while simultaneously forming nutrient channels, could potentially emerge as a strategy for fabricating vascularized tissue-engineered bone. Moreover, bioinks should also be applied in combination to promote both osteogenesis and angiogenesis. In this study, employing triaxial bioprinting, we used a blend bioink of gelatin methacryloyl (GelMA), sodium alginate (Alg), and different concentrations of nano beta-tricalcium phosphate (nano β-TCP) encapsulated MC3T3-E1 preosteoblasts as the outer layer, a mixed bioink of GelMA and Alg loaded with human umbilical vein endothelial cells (HUVEC) as the middle layer, and gelatin as a sacrificial material to form nutrient channels in the inner layer to fabricate vascularized bone constructs simulating the microenvironment for bone and vascular tissues. The results showed that the addition of nano β-TCP could adjust the mechanical, swelling, and degradation properties of the constructs. Biological assessments revealed the cell viability of constructs containing different concentrations of nano β-TCP was higher than 90% on day 7, The cell-laden constructs containing 3% (w/v) nano β-TCP exhibited better osteogenic (higher Alkaline phosphatase activity and larger Osteocalcin positive area) and angiogenic (the gradual increased CD31 positive area) potential. Therefore, using triaxial bioprinting technology and employing GelMA, Alg, and nano β-TCP as bioink components could fabricate vascularized bone tissue constructs, offering a novel strategy for vascularized bone tissue engineering.

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利用三轴生物打印技术制造血管化组织工程骨模型。
骨组织是一种高度血管化的组织。在构建组织工程骨模型时,应仔细考虑构建物的成骨和血管生成能力。然而,制造血管化组织工程骨以促进血管形成和骨生成,同时建立营养通道以促进构建物内部的营养交换,仍然是一项重大挑战。三轴生物打印技术不仅能独立封装不同类型的细胞,还能同时形成营养通道,有可能成为制造血管化组织工程骨的一种策略。此外,生物墨水还应结合使用,以促进成骨和血管生成。在本研究中,我们采用三轴生物打印技术,使用甲基丙烯酰明胶(GelMA)、海藻酸钠(Alg)和不同浓度的纳米β-磷酸三钙(纳米β-TCP)的混合生物墨水包裹 MC3T3-E1 前成骨细胞作为外层、在中间层使用 GelMA 和 Alg 混合生物墨水负载人脐静脉内皮细胞(HUVEC),在内层使用明胶作为牺牲材料形成营养通道,以制造模拟骨和血管组织微环境的血管化骨构建体。结果表明,添加纳米 β-TCP 可调节构建物的机械、膨胀和降解性能。生物学评估显示,含有不同浓度纳米 β-TCP 的构建体在第 7 天的细胞存活率高于 90%,而含有 3%(w/v)纳米 β-TCP 的细胞负载构建体具有更好的成骨(更高的碱性磷酸酶活性和更大的骨钙素阳性面积)和血管生成(CD31 阳性面积逐渐增加)潜力。因此,利用三轴生物打印技术,采用 GelMA、Alg 和纳米 β-TCP 作为生物墨水成分,可以制造出血管化骨组织构建体,为血管化骨组织工程提供了一种新策略。
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来源期刊
Journal of biomedical materials research. Part A
Journal of biomedical materials research. Part A 工程技术-材料科学:生物材料
CiteScore
10.40
自引率
2.00%
发文量
135
审稿时长
3.6 months
期刊介绍: The Journal of Biomedical Materials Research Part A is an international, interdisciplinary, English-language publication of original contributions concerning studies of the preparation, performance, and evaluation of biomaterials; the chemical, physical, toxicological, and mechanical behavior of materials in physiological environments; and the response of blood and tissues to biomaterials. The Journal publishes peer-reviewed articles on all relevant biomaterial topics including the science and technology of alloys,polymers, ceramics, and reprocessed animal and human tissues in surgery,dentistry, artificial organs, and other medical devices. The Journal also publishes articles in interdisciplinary areas such as tissue engineering and controlled release technology where biomaterials play a significant role in the performance of the medical device. The Journal of Biomedical Materials Research is the official journal of the Society for Biomaterials (USA), the Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Articles are welcomed from all scientists. Membership in the Society for Biomaterials is not a prerequisite for submission.
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