ANCUT1, a novel thermoalkaline cutinase from Aspergillus nidulans and its application on hydroxycinnamic acids lipophilization.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology Letters Pub Date : 2024-06-01 Epub Date: 2024-02-28 DOI:10.1007/s10529-024-03467-2

Carolina Peña-Montes, Eva Bermúdez-García, Denise Castro-Ochoa, Fernanda Vega-Pérez, Katia Esqueda-Domínguez, José Augusto Castro-Rodríguez, Augusto González-Canto, Laura Segoviano-Reyes, Arturo Navarro-Ocaña, Amelia Farrés

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Abstract

One of the four cutinases encoded in the Aspergillus nidulans genome, ANCUT1, is described here. Culture conditions were evaluated, and it was found that this enzyme is produced only when cutin is present in the culture medium, unlike the previously described ANCUT2, with which it shares 62% amino acid identity. The differences between them include the fact that ANCUT1 is a smaller enzyme, with experimental molecular weight and pI values of 22 kDa and 6, respectively. It shows maximum activity at pH 9 and 60 °C under assayed conditions and retains more than 60% of activity after incubation for 1 h at 60 °C in a wide range of pH values (6-10) after incubations of 1 or 3 h. It has a higher activity towards medium-chain esters and can modify long-chain length hydroxylated fatty acids constituting cutin. Its substrate specificity properties allow the lipophilization of alkyl coumarates, valuable antioxidants and its thermoalkaline behavior, which competes favorably with other fungal cutinases, suggests it may be useful in many more applications.

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来自裸曲霉的新型热碱性角叉菜酶 ANCUT1 及其在羟基肉桂酸脂化中的应用

本文介绍了裸曲霉基因组中编码的四种角质素酶之一 ANCUT1。对培养条件进行了评估，发现这种酶只有在培养基中存在角叉菜胶时才会产生，这与之前描述的 ANCUT2 不同，ANCUT2 与 ANCUT1 有 62% 的氨基酸相同性。它们之间的区别包括 ANCUT1 是一种较小的酶，实验分子量和 pI 值分别为 22 kDa 和 6。在测定条件下，它在 pH 值为 9 和 60 °C 时显示出最大活性，在 60 °C 下孵育 1 小时后，在广泛的 pH 值范围（6-10）内孵育 1 或 3 小时后仍能保持 60% 以上的活性。它对中链酯具有较高的活性，并能修饰构成角质素的长链羟化脂肪酸。它的底物特异性使烷基香豆酸盐（一种有价值的抗氧化剂）能够亲脂化，而且它的热碱性行为与其他真菌角质酶相比具有优势，这表明它可能会有更多的用途。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.