Early delivery of human umbilical cord mesenchymal stem cells improves healing in a rat model of Achilles tendinopathy.

IF 2.4 4区医学 Q4 CELL & TISSUE ENGINEERING Regenerative medicine Pub Date : 2024-02-01 Epub Date: 2024-02-28 DOI:10.2217/rme-2023-0222

Ze Yuan, Ding Yu, Yanxue Wang, Lijiaqi Liu, Junchao Wang, Chao Ma, Shaoling Wu

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Abstract

Objective: This study aimed to explore the efficacy and optimal delivery time of human umbilical cord mesenchymal stem cells (hUC-MSCs) in treating collagenase-induced Achilles tendinopathy. Methods: Achilles tendinopathy in rats at early or advanced stages was induced by injecting collagenase I into bilateral Achilles tendons. A total of 28 injured rats were injected with a hUC-MSC solution or normal saline into bilateral tendons twice and sampled after 4 weeks for histological staining, gene expression analysis, transmission electron microscope assay and biomechanical testing analysis. Results: The results revealed better histological performance and a larger collagen fiber diameter in the MSC group. mRNA expression of TNF-α, IL-1β and MMP-3 was lower after MSC transplantation. Early MSC delivery promoted collagen I and TIMP-3 synthesis, and strengthened tendon toughness. Conclusion: hUC-MSCs demonstrated a therapeutic effect in treating collagenase-induced Achilles tendinopathy, particularly in the early stage of tendinopathy.

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早期输送人脐带间充质干细胞可改善跟腱病大鼠模型的愈合。

研究目的本研究旨在探讨人脐带间充质干细胞（hUC-MSCs）治疗胶原酶诱导的跟腱病的疗效和最佳给药时间。研究方法通过向双侧跟腱注射胶原酶I，诱导早期或晚期大鼠跟腱病变。对 28 只受伤大鼠的双侧肌腱注射两次 hUC 间充质干细胞溶液或生理盐水，4 周后取样进行组织学染色、基因表达分析、透射电子显微镜检测和生物力学测试分析。结果显示结果显示，间充质干细胞组的组织学表现更好，胶原纤维直径更大；间充质干细胞移植后 TNF-α、IL-1β 和 MMP-3 的 mRNA 表达量更低。早期间充质干细胞的输送促进了胶原蛋白I和TIMP-3的合成，增强了肌腱的韧性。结论：hUC-间充质干细胞对治疗胶原酶诱导的跟腱病具有疗效，尤其是在跟腱病的早期阶段。

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来源期刊

Regenerative medicine 医学-工程：生物医学

CiteScore

4.20

自引率

3.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Regenerative medicine replaces or regenerates human cells, tissue or organs, to restore or establish normal function*. Since 2006, Regenerative Medicine has been at the forefront of publishing the very best papers and reviews covering the entire regenerative medicine sector. The journal focusses on the entire spectrum of approaches to regenerative medicine, including small molecule drugs, biologics, biomaterials and tissue engineering, and cell and gene therapies – it’s all about regeneration and not a specific platform technology. The journal’s scope encompasses all aspects of the sector ranging from discovery research, through to clinical development, through to commercialization. Regenerative Medicine uniquely supports this important area of biomedical science and healthcare by providing a peer-reviewed journal totally committed to publishing the very best regenerative medicine research, clinical translation and commercialization. Regenerative Medicine provides a specialist forum to address the important challenges and advances in regenerative medicine, delivering this essential information in concise, clear and attractive article formats – vital to a rapidly growing, multidisciplinary and increasingly time-constrained community. Despite substantial developments in our knowledge and understanding of regeneration, the field is still in its infancy. However, progress is accelerating. The next few decades will see the discovery and development of transformative therapies for patients, and in some cases, even cures. Regenerative Medicine will continue to provide a critical overview of these advances as they progress, undergo clinical trials, and eventually become mainstream medicine.