Bacteria of the Genus Bacillus on the Russian Segment of the International Space Station

Q3 Agricultural and Biological Sciences Moscow University Biological Sciences Bulletin Pub Date : 2024-03-01 DOI:10.3103/s0096392523700062

R. R. Yenikeyev, L. M. Zakharchuk

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Abstract

Pure cultures of 19 strains of spore-forming bacteria were obtained from the equipment surfaces of the Russian segment of the International Space Station. The study of morphological, cultural, and physiological-biochemical properties of these bacteria allowed the authors to attribute all strains to the genus Bacillus. As a result of using MALDI-TOF methods and genome-wide sequencing, it was found that six of 19 bacillus strains belong to the species B. paralicheniformis, four to B. pumilus, four to B. subtilis, two to B. cereus, and one to B. amyloliquefaciens. In accordance with the requirements and norms of EUCAST 2023, the resistance of bacillus strains obtained from the Russian segment of the International Space Station to such antibiotics as imipenem, meropenem, ciprofloxacin, levofloxacin, norfloxacin, vancomycin, erythromycin, clindamycin, and linezolid was studied. Resistance to erythromycin was found in 11 strains of Bacillus, and five strains showed resistance to clindamycin. Only one strain showed resistance to imipenem, levofloxacin, and norfloxacin, respectively. Analysis of the complete genome of bacterial strains in which resistance to erythromycin and (or) clindamycin was found made it possible to establish that resistance to these antibiotics in B. paralicheniformis strains SE71, SE131, SE181, SE182, and SE183 provides the ermD antibiotic resistance gene. In B. cereus SE43, resistance to erythromycin encodes the mphL gene.

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国际空间站俄罗斯段上的芽孢杆菌属细菌

摘要从国际空间站俄罗斯舱段的设备表面获得了 19 株孢子形成细菌的纯培养物。通过对这些细菌的形态、培养和生理生化特性的研究，作者将所有菌株都归属于芽孢杆菌属。通过使用 MALDI-TOF 方法和全基因组测序，发现 19 株芽孢杆菌中有 6 株属于巴氏副芽孢杆菌属，4 株属于普米勒斯芽孢杆菌属，4 株属于枯草芽孢杆菌属，2 株属于蜡样芽孢杆菌属，1 株属于淀粉样芽孢杆菌属。根据 EUCAST 2023 的要求和规范，研究了从国际空间站俄罗斯舱段获得的杆菌菌株对亚胺培南、美罗培南、环丙沙星、左氧氟沙星、诺氟沙星、万古霉素、红霉素、林可霉素和利奈唑胺等抗生素的耐药性。结果发现，11 株芽孢杆菌对红霉素产生了抗药性，5 株对克林霉素产生了抗药性。只有一株菌株分别对亚胺培南、左氧氟沙星和诺氟沙星产生了耐药性。对发现对红霉素和（或）克林霉素有抗药性的菌株的完整基因组进行分析，可以确定副银状芽孢杆菌 SE71、SE131、SE181、SE182 和 SE183 株对这些抗生素的抗药性由 ermD 抗生素抗性基因提供。在蜡样芽孢杆菌 SE43 中，对红霉素的抗药性由 mphL 基因编码。

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来源期刊

Moscow University Biological Sciences Bulletin Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

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1.00

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期刊介绍： Moscow University Biological Sciences Bulletin is forum for research in all important areas of modern biology. It publishes original work on qualitative, analytical and experimental aspects of research. The scope of articles to be considered includes plant biology, zoology, ecology, evolutionary biology, biophysics, genetics, genomics, proteomics, molecular biology, cell biology, biochemistry, endocrinology, immunology, physiology, pharmacology, neuroscience, gerontology, developmental biology, bioinformatics, bioengineering, virology, and microbiology.