Semi-purified Antimicrobial Proteins from Oyster Hemolymph Inhibit Pneumococcal Infection

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Marine Biotechnology Pub Date : 2024-03-02 DOI:10.1007/s10126-024-10297-w
Kate Summer, Lei Liu, Qi Guo, Bronwyn Barkla, Kirsten Benkendorff
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Abstract

Pneumococcal infections caused by Streptococcus pneumoniae are a leading cause of morbidity and mortality globally, particularly among children. The ability of S. pneumoniae to form enduring biofilms makes treatment inherently difficult, and options are further limited by emerging antibiotic resistance. The discovery of new antibiotics, particularly those with antibiofilm activity, is therefore increasingly important. Antimicrobial proteins and peptides (AMPs) from marine invertebrates are recognised as promising pharmacological leads. This study determined the in vitro antibacterial activity of hemolymph and unique protein fractions from an Australian oyster (Saccostrea glomerata) against multi-drug-resistant S. pneumoniae. We developed a successful method for hemolymph extraction and separation into 16 fractions by preparative HPLC. The strongest activity was observed in fraction 7: at 42 µg/mL protein, this fraction was bactericidal to S. pneumoniae and inhibited biofilm formation. Proteomic analysis showed that fraction 7 contained relatively high abundance of carbonic anhydrase, cofilin, cystatin B–like, and gelsolin-like proteins, while surrounding fractions, which showed lower or no antibacterial activity, contained these proteins in lower abundance or not at all. This work supports traditional medicinal uses of oysters and contributes to further research and development of novel hemolymph/AMP-based treatments for pneumococcal infections.

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牡蛎血淋巴中的半纯化抗菌蛋白可抑制肺炎球菌感染
肺炎链球菌引起的肺炎球菌感染是全球发病和死亡的主要原因,尤其是在儿童中。肺炎链球菌能够形成持久的生物膜,这给治疗带来了固有的困难,而新出现的抗生素耐药性又进一步限制了治疗方案的选择。因此,发现新的抗生素,尤其是具有抗生物膜活性的抗生素,变得越来越重要。来自海洋无脊椎动物的抗菌蛋白和肽(AMPs)被认为是有前景的药理学线索。本研究测定了澳大利亚牡蛎(Saccostrea glomerata)的血淋巴和独特蛋白质组分对耐多药肺炎双球菌的体外抗菌活性。我们开发出了一种成功的血淋巴提取方法,并通过制备型高效液相色谱法将其分离成 16 个馏分。在馏分 7 中观察到了最强的活性:当蛋白质含量为 42 µg/mL 时,该馏分对肺炎双球菌具有杀菌作用,并能抑制生物膜的形成。蛋白质组分析表明,馏分 7 中含有相对较多的碳酸酐酶、cofilin、胱抑素 B 样蛋白和凝胶酶原样蛋白,而周围馏分中的这些蛋白含量较低或根本不含这些蛋白,这些馏分显示出较低的抗菌活性或没有抗菌活性。这项研究支持牡蛎的传统药用价值,并有助于进一步研究和开发基于血淋巴/AMP的新型肺炎球菌感染治疗方法。
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来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
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