Chitosan-inspired Matrices for Folic Acid. Insightful Structural Characterization and Ensured Bioaccessibility

IF 2.8 4区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Food Biophysics Pub Date : 2024-03-01 DOI:10.1007/s11483-024-09833-x

Regina De Matteo, Juan M. Rey, Rocío Corfield, Victoria A. Gómez Andrade, Patricio R. Santagapita, Florencia Di Salvo, Oscar E. Pérez

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Abstract

The objective of this research was to obtain and characterize chitosan (CS) based matrices conceived as folic acid (FA) dried reservoir that could find application as food coating upon rehydration. An Argentinean and high molecular weight (MW) chitosan (AC) was used and contrasted with a commercial type one (CC). FTIR and XRD were applied for the polysaccharide-vitamin dried mixed systems characterization. Shifts in bands of FA and AC or CC were observed in the mixed systems, confirming the interaction among the components, for example, the bands at 1382 and 1391 cm^− 1 (AC and FA, respectively) shifted to 1387 cm^–1 in the in AC-FA mixed systems. XRD data showed that the FA is in its protonated form as expected and is maintained in the different mixed systems obtained using either with CC or AC, demonstrating the robustness and applicability of the material to properly conserve the active ingredient.

The ultrastructure and topography were studied by SEM and AFM, respectively. FA incrustations in a self-associated form were observed in the topography images. Variations in topography observed for the AC-FA and CC-FA mixed systems could be attributed to specific interactions between the components and the molecular features of each CS (i.e. the mean particle size was 63 ± 3 and 9 ± 1 nm and, roughness gave values of 0.47 and 0.41 nm, respectively).

Vitamin bioaccessibility remained unchanged compared to the control (88.36% ±10.35%), being equal to 91.08% ± 5.02% for AC-FA and 71.56% ±14.08% for CC-FA, after submitted samples to an in vitro digestion process. In sum, mixed AC-FA systems obtained are promising materials for a possible additional application in coating matrices inspired by biopolymers to improve food preservation, maintaining the bioaccessibility of vitamins.

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壳聚糖启发的叶酸基质。深入的结构表征和确保生物可及性

摘要这项研究的目的是获得和表征壳聚糖（CS）基质，并将其设想为叶酸（FA）干燥储层，可在再水化后用作食品涂层。研究中使用了阿根廷产的高分子量壳聚糖（AC），并将其与商用壳聚糖（CC）进行对比。傅立叶变换红外光谱（FTIR）和 X 射线衍射（XRD）被用于多糖-维生素干混合体系的表征。在混合体系中观察到 FA 和 AC 或 CC 的条带发生了移动，证实了各组分之间的相互作用，例如，在 AC-FA 混合体系中，1382 和 1391 cm- 1（分别为 AC 和 FA）处的条带移动到了 1387 cm-1。XRD 数据显示，在使用 CC 或 AC 得到的不同混合体系中，FA 如预期的那样处于质子化形式，并保持不变，这证明了该材料在适当保存活性成分方面的稳健性和适用性。扫描电镜和原子力显微镜分别对超微结构和形貌进行了研究。在形貌图像中观察到了自结合形式的 FA 结壳。在 AC-FA 和 CC-FA 混合体系中观察到的形貌变化可归因于成分之间的特定相互作用和每种 CS 的分子特征（即平均粒径分别为 63 ± 3 和 9 ± 1 nm，粗糙度值分别为 0.47 和 0.41 nm）。与对照组（88.36%±10.35%）相比，维生素的生物可及性保持不变，经过体外消化过程后，AC-FA 的生物可及性为 91.08% ± 5.02%，CC-FA 的生物可及性为 71.56% ± 14.08%。总之，所获得的 AC-FA 混合体系是一种很有前景的材料，有可能进一步应用于受生物聚合物启发的涂层基质中，以改善食品保存，保持维生素的生物可及性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Biophysics 工程技术-食品科技

CiteScore

5.80

自引率

3.30%

发文量

审稿时长

1 months

期刊介绍： Biophysical studies of foods and agricultural products involve research at the interface of chemistry, biology, and engineering, as well as the new interdisciplinary areas of materials science and nanotechnology. Such studies include but are certainly not limited to research in the following areas: the structure of food molecules, biopolymers, and biomaterials on the molecular, microscopic, and mesoscopic scales; the molecular basis of structure generation and maintenance in specific foods, feeds, food processing operations, and agricultural products; the mechanisms of microbial growth, death and antimicrobial action; structure/function relationships in food and agricultural biopolymers; novel biophysical techniques (spectroscopic, microscopic, thermal, rheological, etc.) for structural and dynamical characterization of food and agricultural materials and products; the properties of amorphous biomaterials and their influence on chemical reaction rate, microbial growth, or sensory properties; and molecular mechanisms of taste and smell. A hallmark of such research is a dependence on various methods of instrumental analysis that provide information on the molecular level, on various physical and chemical theories used to understand the interrelations among biological molecules, and an attempt to relate macroscopic chemical and physical properties and biological functions to the molecular structure and microscopic organization of the biological material.