Crocetin protects mouse brain from apoptosis in traumatic brain injury model through activation of autophagy.

IF 1.5 4区医学 Q4 NEUROSCIENCES Brain injury Pub Date : 2024-06-06 Epub Date: 2024-03-03 DOI:10.1080/02699052.2024.2324022

Shan Chen, Xinghong Luo, Liu Yang, Liang Luo, Zhen Hu, Jianglan Wang

{"title":"Crocetin protects mouse brain from apoptosis in traumatic brain injury model through activation of autophagy.","authors":"Shan Chen, Xinghong Luo, Liu Yang, Liang Luo, Zhen Hu, Jianglan Wang","doi":"10.1080/02699052.2024.2324022","DOIUrl":null,"url":null,"abstract":"Background: Autophagy is recognized as a promising therapeutic target for traumatic brain injury (TBI). Crocetin is an aglycone of crocin naturally occurring in saffron and has been found to alleviate brain injury diseases. However, whether crocetin affects autophagy after TBI remains unknown. Therefore, we explore crocetin roles in autophagy after TBI.Methods: We used a weight-dropped model to induce TBI in C57BL/6J mice. Neurological severity scoring (NSS) and grip tests were used to evaluate the neurological level of injury. Brain edema, neuronal apoptosis, neuroinflammation and autophagy were detected by measurements of brain water content, TUNEL staining, ELISA kits and western blotting.Results: Crocetin ameliorated neurological dysfunctions and brain edema after TBI. Crocetin reduced neuronal apoptosis and neuroinflammation and enhanced autophagy after TBI.Conclusion: Crocetin alleviates TBI by inhibiting neuronal apoptosis and neuroinflammation and activating autophagy.","PeriodicalId":9082,"journal":{"name":"Brain injury","volume":" ","pages":"524-530"},"PeriodicalIF":1.5000,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brain injury","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/02699052.2024.2324022","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/3/3 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"NEUROSCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Autophagy is recognized as a promising therapeutic target for traumatic brain injury (TBI). Crocetin is an aglycone of crocin naturally occurring in saffron and has been found to alleviate brain injury diseases. However, whether crocetin affects autophagy after TBI remains unknown. Therefore, we explore crocetin roles in autophagy after TBI.

Methods: We used a weight-dropped model to induce TBI in C57BL/6J mice. Neurological severity scoring (NSS) and grip tests were used to evaluate the neurological level of injury. Brain edema, neuronal apoptosis, neuroinflammation and autophagy were detected by measurements of brain water content, TUNEL staining, ELISA kits and western blotting.

Results: Crocetin ameliorated neurological dysfunctions and brain edema after TBI. Crocetin reduced neuronal apoptosis and neuroinflammation and enhanced autophagy after TBI.

Conclusion: Crocetin alleviates TBI by inhibiting neuronal apoptosis and neuroinflammation and activating autophagy.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

藏红花酸通过激活自噬保护创伤性脑损伤模型中的小鼠大脑免受凋亡。

背景：自噬被认为是治疗创伤性脑损伤（TBI）的一个有前景的靶点。藏红花素是藏红花中天然存在的一种藏红花苷的苷元，已被发现能缓解脑损伤疾病。然而，西红花素是否会影响创伤性脑损伤后的自噬仍然是个未知数。因此，我们探讨了藏红花苷在创伤后自噬中的作用：方法：我们使用体重下降模型诱导 C57BL/6J 小鼠发生创伤性脑损伤。方法：我们采用体重下降模型诱导 C57BL/6J 小鼠发生创伤性脑损伤，并使用神经系统严重程度评分（NSS）和握力测试来评估神经系统的损伤程度。通过测量脑含水量、TUNEL染色、ELISA试剂盒和Western印迹检测脑水肿、神经元凋亡、神经炎症和自噬：结果：rocetin能改善创伤性脑损伤后的神经功能障碍和脑水肿。结果：茜草素能改善创伤性脑损伤后的神经功能紊乱和脑水肿，减少神经元凋亡和神经炎症，增强自噬作用：结论：茜草素能抑制神经元凋亡和神经炎症，激活自噬，从而缓解创伤性脑损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Brain injury 医学-康复医学

CiteScore

3.50

自引率

5.30%

发文量

148

审稿时长

12 months

期刊介绍： Brain Injury publishes critical information relating to research and clinical practice, adult and pediatric populations. The journal covers a full range of relevant topics relating to clinical, translational, and basic science research. Manuscripts address emergency and acute medical care, acute and post-acute rehabilitation, family and vocational issues, and long-term supports. Coverage includes assessment and interventions for functional, communication, neurological and psychological disorders.