Blastocyst formation in vitrified-warmed preimplantation embryos derived from vitrified-warmed oocytes in a mouse model.

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-03-01 Epub Date: 2024-02-29 DOI:10.5653/cerm.2023.06499
Yeon Hee Hong, Byung Chul Jee
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Abstract

Objective: The purpose of this study was to use a mouse model to investigate the blastocyst formation rate in vitrified-warmed embryos derived from vitrified-warmed oocytes.

Methods: Metaphase II oocytes obtained from BDF1 mice were vitrified and warmed, followed by fertilization with epididymal sperm. On day 3, a total of 176 embryos, at either the eight-cell or the morula stage, were vitrified-warmed (representing group 1). For group 2, 155 embryos at the same developmental stages were not vitrified, but rather were directly cultured until day 5. Finally, group 3 included day-5 blastocysts derived from fresh oocytes, which served as fresh controls. The primary outcome measured was the rate of blastocyst formation per day-3 embryo at the eight-cell or morula stage.

Results: The rates of blastocyst formation per day-3 embryo were comparable between groups 1 and 2, at 64.5% and 69.7%, respectively (p>0.05). The formation rates of good-quality blastocysts (expanded, hatching, or hatched) were also similar for groups 1 and 2, at 35.5% and 43.2%, respectively (p>0.05). For the fresh oocytes (group 3), the blastocyst formation rate was 75.5%, which was similar to groups 1 and 2. However, the rate of good-quality blastocyst formation in group 3 was 57.3%, significantly exceeding those of group 1 (p=0.001) and group 2 (p=0.023).

Conclusion: Regarding developmental potential to the blastocyst stage, vitrified-warmed day-3 embryos originating from vitrified-warmed oocytes demonstrated comparable results to non-vitrified embryos from similar oocytes. These findings indicate that day-3 embryos derived from vitrified-warmed oocytes can be effectively cryopreserved without incurring cellular damage.

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在小鼠模型中,从玻璃化温育卵母细胞中提取的玻璃化温育植入前胚胎的囊胚形成。
研究目的本研究的目的是利用小鼠模型研究玻璃化温育卵母细胞所产生的玻璃化温育胚胎的囊胚形成率:方法:将从 BDF1 小鼠获得的二分裂期卵母细胞进行玻璃化和温育,然后用附睾精子受精。第 3 天,玻璃化-温育的胚胎共有 176 个,分别处于八细胞期或蜕膜期(代表第 1 组)。在第 2 组中,处于相同发育阶段的 155 个胚胎未进行玻璃化处理,而是直接培养至第 5 天。最后,第 3 组包括来自新鲜卵母细胞的第 5 天囊胚,作为新鲜对照组。测量的主要结果是每个第 3 天胚胎在 8 细胞或 morula 阶段的囊胚形成率:结果:第 1 组和第 2 组第 3 天胚胎的囊胚形成率相当,分别为 64.5%和 69.7%(P>0.05)。第 1 组和第 2 组的优质囊胚(膨大、孵化或孵化)形成率也相似,分别为 35.5% 和 43.2%(p>0.05)。新鲜卵母细胞(第 3 组)的囊胚形成率为 75.5%,与第 1 组和第 2 组相似。然而,第 3 组的优质囊胚形成率为 57.3%,明显高于第 1 组(p=0.001)和第 2 组(p=0.023):结论:在囊胚阶段的发育潜力方面,来自玻璃化温育卵母细胞的玻璃化温育第 3 天胚胎与来自类似卵母细胞的非玻璃化胚胎结果相当。这些研究结果表明,来自玻璃化温育卵母细胞的第 3 天胚胎可以有效冷冻保存而不会造成细胞损伤。
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