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Pathological and vascular changes in the rat testiсle after experimental trauma. 实验性创伤后大鼠睾丸的病理和血管变化
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-09-20 DOI: 10.5653/cerm.2024.07080
Anastasiya Spaska, Bogdan Grytsuliak, Nelia Dolynko

Objective: Mechanical trauma to the testicles poses a potential risk of tissue destruction, disruption of local blood supply, and impairment of spermatogenesis, which can ultimately lead to infertility. Therefore, investigating this topic is crucial. The study aimed to identify cytological and morphological changes in the testicular tissue of laboratory rats following mechanical trauma to the organ.

Methods: Observations were recorded on days 7, 14, 30, and 90 post-trauma. The experiment involved two groups of animals: a control group of healthy animals and an experimental group that sustained blunt mechanical trauma. Tissue samples were collected, fixed, dehydrated, and embedded in paraffin; subsequently, sections were prepared and stained. Structural changes in tissues and cells were documented using light and transmission electron microscopy.

Results: In the experimental sample, notable changes included a decrease in organ weight, thickening of the protein shell and tubule walls, sclerotisation of the tubule membrane, narrowing of tubule diameter, reduced spermatozoa and spermatids titre, diminished capillary network and spermatogenic epithelium, uneven blood vessel lumen expansion, and decreased volume of Leydig cell nuclei. Additionally, in cells under different functional loads, the cytoplasm was vacuolated, mitochondrial cristae and the Golgi apparatus were diminished, cytoplasm volume decreased, karyopyknosis was observed, and uncharacteristic protrusions appeared on the surface of the cytoplasmic membrane. The severity of destruction at the cellular and tissue levels showed a positive correlation with time.

Conclusion: The data obtained from these model sites can be predictive for clinical trials.

目的:睾丸受到机械性创伤可能会造成组织破坏、局部供血中断和精子生成障碍,最终导致不育。因此,研究这一课题至关重要。本研究旨在确定实验鼠睾丸组织在受到机械性创伤后的细胞学和形态学变化:方法:在创伤后第 7、14、30 和 90 天进行观察记录。实验包括两组动物:健康动物对照组和遭受钝性机械创伤的实验组。采集的组织样本经固定、脱水和石蜡包埋后,制备切片并染色。使用光镜和透射电子显微镜记录组织和细胞的结构变化:结果:在实验样本中,明显的变化包括器官重量减轻、蛋白外壳和小管壁增厚、小管膜硬化、小管直径变窄、精子和精子滴度降低、毛细血管网和生精上皮减少、血管腔扩张不均匀以及莱迪格细胞核体积缩小。此外,在不同功能负荷下的细胞中,细胞质呈空泡化,线粒体嵴和高尔基体减少,细胞质体积缩小,出现核分裂,细胞质膜表面出现不典型的突起。细胞和组织层面的破坏严重程度与时间呈正相关:结论:从这些模型部位获得的数据对临床试验具有预测作用。
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引用次数: 0
Does coenzyme Q10 supplementation protect spermatogenesis in ciprofloxacin-induced rat testes? 补充辅酶Q10是否能保护环丙沙星诱导的大鼠睾丸的精子发生?
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-09-20 DOI: 10.5653/cerm.2024.07017
Elham Aliabadi, Fateme Amooei, Tahereh Talaei Khozani, Saied Karbalay-Doust, Fatemeh Karimi

Objective: Ciprofloxacin (CPFX) is frequently prescribed by fertility specialists and urologists to manage infections in male reproductive organs. However, it is toxic to the testicles and can lead to infertility. Dietary antioxidants are known to protect the testis from damage. This study aimed to investigate the effects of coenzyme Q10 (CoQ10) on the adverse side effects of CPFX using stereological methods.

Methods: Sixty rats were divided into six groups: control (distilled water), CoQ10 (10 mg/kg/day), and low-dose (103 mg/kg/day) and high-dose (206 mg/kg/day) of CPFX (LD-CPFX, HD-CPFX) with or without CoQ10 consumption. The treatments lasted for 45 days. Sperm count, serum testosterone levels, and testicular parameters were evaluated.

Results: Significant decreases in sperm count, motility, normal morphology, viability, and testosterone levels were observed in the LD-CPFX (p<0.003) and HD-CPFX- treated rats (p=0.0001) compared to the control groups. A 10% to 36% reduction in the volume of seminiferous tubules, tubular epithelium, and tubule length was noted in LD-CPFX (p<0.01) and HD-CPFX-treated rats (p<0.006), while the volume of the interstitium increased by 25% to 28% in LD-CPFX (p=0.03) and HD-CPFX (p=0.008) groups. The number of cells, including spermatogonia, spermatocytes, spermatids, Sertoli cells, and Leydig cells, decreased by 36% to 75% in the testes exposed to LD-CPFX (p<0.04) and HD-CPFX (p<0.01), compared to the control groups. However, these changes normalized in rats that received CoQ10.

Conclusion: CPFX exposure for 45 days, regardless of the dose, has detrimental effects on testicular parameters. CoQ10 can prevent CPFX-induced testicular structural impairments.

目的:环丙沙星(CPFX生育专科医生和泌尿科医生经常开环丙沙星(CPFX)来治疗男性生殖器官感染。然而,环丙沙星对睾丸有毒性,可导致不育。众所周知,膳食中的抗氧化剂可保护睾丸免受损伤。本研究旨在采用立体学方法研究辅酶Q10(CoQ10)对CPFX不良副作用的影响:将60只大鼠分为6组:对照组(蒸馏水)、辅酶Q10组(10毫克/千克/天)、服用或不服用辅酶Q10的低剂量(103毫克/千克/天)和高剂量(206毫克/千克/天)CPFX组(LD-CPFX、HD-CPFX)。治疗持续 45 天。对精子数量、血清睾酮水平和睾丸参数进行了评估:结果:在 LD-CPFX 试验中观察到精子数量、活力、正常形态、存活率和睾酮水平显著下降(p):暴露于 CPFX 45 天,无论剂量大小,都会对睾丸参数产生不利影响。CoQ10 可预防 CPFX 引起的睾丸结构损伤。
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引用次数: 0
State-of-the-art in high throughput organ-on-chip for biotechnology and pharmaceuticals. 用于生物技术和制药的高通量片上器官的最新技术。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-09-20 DOI: 10.5653/cerm.2024.06954
Suk-Heung Song, Sehoon Jeong

Modern drug discovery is driven by high demand in the pharmaceutical industry to test growing libraries of compounds against potential targets. High-throughput screening (HTS) is characterized by fully automated experimentation that leverages robotic liquid handling systems, analytical techniques, and advanced computing and statistics, including the recent integration of artificial intelligence. To align with this trend, it is crucial to develop and implement new HTS platforms that offer improved predictivity and physiological relevance. In recent years, microphysiological systems, commonly known as organ-on-chip (OoC) systems, have progressed from a theoretical concept to a powerful alternative to conventional in vitro and animal models. High-throughput OoC (HT-OoC) systems could represent the disruptive technology sought by pharmaceutical companies to address their enormous research and development (R&D) expenses. In this study, we provide a brief overview of commercial products utilizing modern HT-OoC systems in drug discovery and development. Additionally, we discuss recent trends in R&D aimed at industrialization.

现代药物发现的驱动力来自于制药业对针对潜在靶点测试不断增长的化合物库的高需求。高通量筛选(HTS)的特点是利用机器人液体处理系统、分析技术以及先进的计算和统计技术(包括最近整合的人工智能)进行全自动实验。为顺应这一趋势,开发和实施新的高通量筛选平台至关重要,这些平台可提供更好的预测性和生理相关性。近年来,微生理系统(通常称为片上器官(OoC)系统)已从理论概念发展成为传统体外和动物模型的有力替代品。高通量 OoC(HT-OoC)系统可能是制药公司为解决其巨额研发(R&D)费用而寻求的颠覆性技术。在本研究中,我们简要介绍了利用现代 HT-OoC 系统进行药物发现和开发的商业产品。此外,我们还讨论了旨在实现产业化的最新研发趋势。
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引用次数: 0
Metabolomic profiling of embryo culture media in patients with repeated implantation failure during assisted reproductive technology cycles. 辅助生殖技术周期中反复植入失败患者的胚胎培养基代谢组学分析。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-09-01 Epub Date: 2024-04-11 DOI: 10.5653/cerm.2023.06429
Sheyda Nami, Azam Govahi, Nabaa Najjar, Samaneh Ghasemi, Farshid Rezaei, Fatemehsadat Amjadi, Gholamreza Taheripak

Objective: This study investigated the metabolic status of the spent culture media from embryos of patients with repeated implantation failure (RIF) undergoing in vitro fertilization-intracytoplasmic sperm injection cycles in comparison with the embryos from healthy fertile women.

Methods: Metabolite levels in spent culture media were assessed and compared between embryos from RIF patients (n=35) and oocyte donors as controls (n=15). Protein levels of insulin-like growth factor 1 (IGF-1) were determined using Western blotting. Concentrations of glucose, pyruvate, and lactate were measured using spectrophotometry. Ionic colorimetric assay kits were utilized to analyze the concentrations of sodium, chloride, calcium, and magnesium ions. High-performance liquid chromatography was employed to measure the concentrations of glutamic acid, aspartic acid, methionine, phenylalanine, and histidine.

Results: Glucose consumption and lactate secretion were higher in the control group than in the RIF group. The magnesium concentration was significantly higher in the control group than in the RIF group, but glutamic acid and aspartic acid concentrations were lower in the control group than in the RIF patients (p<0.05). The levels of IGF-1, sodium, calcium, chloride, methionine, histidine, and phenylalanine did not show statistically significant differences between the two groups.

Conclusion: The metabolic profile of the culture medium of the embryos in the RIF group differed from that of the control group. These findings suggest potential factors that may affect implantation capacity in RIF patients and provide a new perspective on embryo selection.

研究目的本研究调查了体外受精-卵胞浆内单精子注射周期中反复植入失败(RIF)患者胚胎与健康育龄妇女胚胎废培养基的代谢状况:方法:评估用过的培养基中的代谢物水平,并将 RIF 患者的胚胎(35 个)与作为对照的卵母细胞捐献者的胚胎(15 个)进行比较。用 Western 印迹法测定胰岛素样生长因子 1 (IGF-1) 蛋白水平。使用分光光度法测量葡萄糖、丙酮酸和乳酸的浓度。离子比色分析试剂盒用于分析钠离子、氯离子、钙离子和镁离子的浓度。采用高效液相色谱法测量谷氨酸、天冬氨酸、蛋氨酸、苯丙氨酸和组氨酸的浓度:结果:对照组的葡萄糖消耗量和乳酸分泌量均高于 RIF 组。对照组的镁浓度明显高于 RIF 组,但对照组的谷氨酸和天冬氨酸浓度低于 RIF 患者(p):RIF组胚胎培养基的代谢情况与对照组不同。这些发现提示了可能影响 RIF 患者植入能力的潜在因素,并为胚胎选择提供了新的视角。
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引用次数: 0
In vivo and in vitro sperm production: an overview of the challenges and advances in male fertility restoration. 体内和体外精子生成:男性生育能力恢复的挑战和进展概览。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-09-01 Epub Date: 2024-03-25 DOI: 10.5653/cerm.2023.06569
Zahra Bashiri, Seyed Jamal Hosseini, Maryam Salem, Morteza Koruji

Male infertility can be caused by genetic anomalies, endocrine disorders, inflammation, and exposure to toxic chemicals or gonadotoxic treatments. Therefore, several recent studies have concentrated on the preservation and restoration of fertility to enhance the quality of life for affected individuals. It is currently recommended to biobank the tissue extracted from testicular biopsies to provide a later source of spermatogonial stem cells (SSCs). Another successful approach has been the in vitro production of haploid male germ cells. The capacity of SSCs to transform into sperm, as in testicular tissue transplantation, SSC therapy, and in vitro or ex vivo spermatogenesis, makes them ideal candidates for in vivo fertility restoration. The transplantation of SSCs or testicular tissue to regenerate spermatogenesis and create embryos has been achieved in nonhuman mammal species. Although the outcomes of human trials have yet to be released, this method may soon be approved for clinical use in humans. Furthermore, regenerative medicine techniques that develop tissue or cells on organic or synthetic scaffolds enriched with bioactive molecules have also gained traction. All of these methods are now in different stages of experimentation and clinical trials. However, thanks to rigorous studies on the safety and effectiveness of SSC-based reproductive treatments, some of these techniques may be clinically available in upcoming decades.

男性不育症可由遗传异常、内分泌失调、炎症、接触有毒化学物质或接受性腺毒性治疗引起。因此,最近的一些研究集中于保留和恢复生育能力,以提高患者的生活质量。目前建议将从睾丸活检中提取的组织建立生物库,以便日后提供精原干细胞(SSCs)来源。另一种成功的方法是体外培养单倍体男性生殖细胞。在睾丸组织移植、造血干细胞治疗、体外或体内精子发生过程中,造血干细胞具有转化为精子的能力,这使它们成为体内生育力恢复的理想候选者。移植造血干细胞或睾丸组织以再生精子和制造胚胎的方法已在非人类哺乳动物物种中实现。虽然人类试验的结果尚未公布,但这种方法可能很快就会被批准用于人类临床。此外,在富含生物活性分子的有机或合成支架上培育组织或细胞的再生医学技术也受到了广泛关注。所有这些方法目前都处于不同的实验和临床试验阶段。不过,由于对基于造血干细胞的生殖治疗的安全性和有效性进行了严格研究,其中一些技术可能会在未来几十年内应用于临床。
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引用次数: 0
Outcomes of female reproductive performance with assisted reproductive techniques after recent mild to moderate COVID-19 infections: An observational study. 近期轻度至中度 COVID-19 感染后使用辅助生殖技术的女性生殖效果:一项观察性研究。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-09-01 Epub Date: 2024-03-25 DOI: 10.5653/cerm.2023.06352
Ashraf Moini, Narges Najafpour, Ladan Kashani, Maryam Farid-Mojtahedi, Arezoo Maleki-Hajiagha, Afsaneh Tehranian, Rana Karimi

Objective: The purpose of this study was to evaluate the impact of preimplantation genetic testing for aneuploidy (PGT-A) on clinical outcomes among high-risk patients.

Methods: This retrospective study involved 1,368 patients and the same number of cycles, including 520 cycles with PGT-A and 848 cycles without PGT-A. The study participants comprised women of advanced maternal age (AMA) and those affected by recurrent implantation failure (RIF), recurrent pregnancy loss (RPL), or severe male factor infertility (SMF).

Results: PGT-A was associated with significant improvements in the implantation rate (IR) and the ongoing pregnancy rate/live birth rate (OPR/LBR) per embryo transfer cycle in the AMA (39.3% vs. 16.2% [p<0.001] and 42.0% vs. 21.8% [p<0.001], respectively), RIF (41.7% vs. 22.0% [p<0.001] and 47.0% vs. 28.6% [p<0.001], respectively), and RPL (45.6% vs. 19.5% [p<0.001] and 49.1% vs. 24.2% [p<0.001], respectively) groups, as well as the IR in the SMF group (43.3% vs. 26.5%, p=0.011). Additionally, PGT-A was associated with lower overall incidence rates of pregnancy loss in the AMA (16.7% vs. 34.3%, p=0.001) and RPL (16.7% vs. 50.0%, p<0.001) groups. However, the OPR/LBR per total cycle across all PGT-A groups did not significantly exceed that for the control groups.

Conclusion: PGT-A demonstrated beneficial effects in high-risk patients. However, our findings indicate that these benefits are more pronounced in carefully selected candidates than in the entire high-risk patient population.

研究目的本研究旨在评估植入前非整倍体基因检测(PGT-A)对高风险患者临床结果的影响:这项回顾性研究涉及 1368 名患者和相同数量的周期,其中 520 个周期进行了 PGT-A,848 个周期未进行 PGT-A。研究参与者包括高龄产妇(AMA)和受复发性着床失败(RIF)、复发性妊娠丢失(RPL)或严重男性因素不育(SMF)影响的妇女:结果:PGT-A 能显著提高 AMA 患者每个胚胎移植周期的植入率(IR)和持续妊娠率/活产率(OPR/LBR)(39.3% vs. 16.2% [p结论:PGT-A 能显著提高 AMA 患者每个胚胎移植周期的植入率(IR)和持续妊娠率/活产率(OPR/LBR):PGT-A 对高风险患者有益处。然而,我们的研究结果表明,与整个高危患者群体相比,这些益处在精心挑选的候选者中更为明显。
{"title":"Outcomes of female reproductive performance with assisted reproductive techniques after recent mild to moderate COVID-19 infections: An observational study.","authors":"Ashraf Moini, Narges Najafpour, Ladan Kashani, Maryam Farid-Mojtahedi, Arezoo Maleki-Hajiagha, Afsaneh Tehranian, Rana Karimi","doi":"10.5653/cerm.2023.06352","DOIUrl":"10.5653/cerm.2023.06352","url":null,"abstract":"<p><strong>Objective: </strong>The purpose of this study was to evaluate the impact of preimplantation genetic testing for aneuploidy (PGT-A) on clinical outcomes among high-risk patients.</p><p><strong>Methods: </strong>This retrospective study involved 1,368 patients and the same number of cycles, including 520 cycles with PGT-A and 848 cycles without PGT-A. The study participants comprised women of advanced maternal age (AMA) and those affected by recurrent implantation failure (RIF), recurrent pregnancy loss (RPL), or severe male factor infertility (SMF).</p><p><strong>Results: </strong>PGT-A was associated with significant improvements in the implantation rate (IR) and the ongoing pregnancy rate/live birth rate (OPR/LBR) per embryo transfer cycle in the AMA (39.3% vs. 16.2% [p<0.001] and 42.0% vs. 21.8% [p<0.001], respectively), RIF (41.7% vs. 22.0% [p<0.001] and 47.0% vs. 28.6% [p<0.001], respectively), and RPL (45.6% vs. 19.5% [p<0.001] and 49.1% vs. 24.2% [p<0.001], respectively) groups, as well as the IR in the SMF group (43.3% vs. 26.5%, p=0.011). Additionally, PGT-A was associated with lower overall incidence rates of pregnancy loss in the AMA (16.7% vs. 34.3%, p=0.001) and RPL (16.7% vs. 50.0%, p<0.001) groups. However, the OPR/LBR per total cycle across all PGT-A groups did not significantly exceed that for the control groups.</p><p><strong>Conclusion: </strong>PGT-A demonstrated beneficial effects in high-risk patients. However, our findings indicate that these benefits are more pronounced in carefully selected candidates than in the entire high-risk patient population.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11372315/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Potential ameliorative effects of bilberry (Vaccinium myrtillus L.) fruit extract on cisplatin-induced reproductive damage in adult male albino rats. 山桑子(Vaccinium myrtillus L.)果实提取物对顺铂诱导的成年雄性白化大鼠生殖损伤的潜在改善作用
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-09-01 Epub Date: 2024-04-12 DOI: 10.5653/cerm.2023.06380
Fatma B Mossa, Nadia Bakry, Mamdouh Rashad El-Sawi

Objective: Cisplatin (CP) is a widely used chemotherapeutic agent, but its severe side effects impact testicular function. We investigated the potential protective effects of bilberry extract against CP-induced testicular toxicity.

Methods: Forty adult male albino rats were divided into four groups. Control animals received a single oral dose of 0.9% saline. Bilberry-treated rats received oral bilberry extract (200 mg/kg body weight [BW] dissolved in 1 mL of saline) daily for 10 consecutive days. CP-treated animals were administered a single intraperitoneal dose (7.5 mg/kg BW). Finally, a bilberry+CP group received oral bilberry extract (200 mg/kg BW) daily for 10 consecutive days, with one intraperitoneal dose of CP (7.5 mg/kg BW) on day 2. We assessed sperm count, motility, viability, and abnormalities, along with testis weight, testis weight-to-BW ratio, antioxidant activity, levels of oxidative stress markers (malondialdehyde [MDA] and hydrogen peroxide [H2O2]), sex hormones (follicle-stimulating hormone [FSH], luteinizing hormone [LH], and testosterone), and apoptotic and anti-apoptotic markers, and DNA damage. Testicular tissue underwent histopathological examination.

Results: Among CP-treated rats, significantly lower values were observed for testis weight; testis weight-to-BW ratio; levels of FSH, LH, testosterone, superoxide dismutase, catalase, glutathione S-transferase, glutathione, and B-cell lymphoma 2; and sperm count, motility, and proportion of normal sperm. CP administration was associated with higher MDA, H2O2, p53, Bax, cytochrome c, caspase 9, and caspase 3 levels, along with elevated tail moment. However, bilberry extract administration significantly improved all altered parameters.

Conclusion: Bilberry treatment demonstrated protective effects and reduced CP-induced testicular toxicity via antioxidant activity and cytoprotection.

目的:顺铂(CP)是一种广泛使用的化疗药物,但其严重的副作用会影响睾丸功能。我们研究了山桑子提取物对顺铂引起的睾丸毒性的潜在保护作用:方法:40 只成年雄性白化大鼠分为四组。对照组动物口服单剂量 0.9% 生理盐水。山桑子处理的大鼠连续 10 天每天口服山桑子提取物(200 毫克/千克体重[BW] 溶于 1 毫升生理盐水)。氯化石蜡处理组大鼠腹腔注射一次氯化石蜡(7.5 毫克/千克体重)。最后,山桑子 + 氯化石蜡组连续 10 天每天口服山桑子提取物(200 毫克/千克体重),并在第 2 天腹腔注射一次氯化石蜡(7.5 毫克/千克体重)。我们评估了精子数量、活力、存活率和畸形率、睾丸重量、睾丸重量与体重之比、抗氧化活性、氧化应激标记物(丙二醛 [MDA] 和过氧化氢 [H2O2])水平、性激素(卵泡刺激素 [FSH]、黄体生成素 [LH] 和睾酮)、凋亡和抗凋亡标记物以及 DNA 损伤。睾丸组织接受了组织病理学检查:结果:在服用过氯化石蜡的大鼠中,睾丸重量、睾丸重量与体重之比、FSH、LH、睾酮、超氧化物歧化酶、过氧化氢酶、谷胱甘肽 S-转移酶、谷胱甘肽和 B 细胞淋巴瘤 2 的水平,以及精子数量、活力和正常精子的比例都明显降低。服用氯化石蜡与较高的 MDA、H2O2、p53、Bax、细胞色素 c、caspase 9 和 caspase 3 水平以及尾矩升高有关。然而,服用山桑子提取物可明显改善所有改变的参数:结论:山桑子治疗具有保护作用,可通过抗氧化活性和细胞保护降低氯化石蜡诱导的睾丸毒性。
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引用次数: 0
Protective effect of platelet-rich plasma against structural and functional changes of the adult rat testis in carbimazole-induced hypothyroidism. 富血小板血浆对卡比马唑诱发甲状腺功能减退症的成年大鼠睾丸结构和功能变化的保护作用
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-09-01 Epub Date: 2024-04-11 DOI: 10.5653/cerm.2023.06695
Hossein Bordbar, Masoud Sattar-Shamsabadi, Farzaneh Dehghani, Fatemeh Karimi

Objective: Hypothyroidism (HT) influences spermatogenesis and is associated with male infertility. Platelet-rich plasma (PRP), a biological product rich in growth factors, promotes tissue repair. In this study, the likely protective effects of PRP on testicular tissue damage in carbimazole (CBZ)-induced HT were evaluated.

Methods: Forty male rats were divided into four groups. HT was induced by administering CBZ (1.35 mg/kg orally, for 45 days). Two doses of PRP (40 μL each, locally injected into the testis on days 15 and 30) were also given. After 45 days, blood samples were taken from the heart to measure triiodothyronine (T3), thyroxine (T4), and testosterone levels, and semen analysis was performed. For stereological assessment, the left testis was removed, fixed, embedded, sectioned, and stained with hematoxylin and eosin. The right testis was excised to evaluate antioxidant levels.

Results: CBZ was demonstrated to induce HT, characterized by significant reductions in T3 and T4. HT was associated with decreased testicular weight, impaired sperm parameters, reduced testosterone concentration, diminished antioxidant activity, reduced volumes of testicular components, and lower total numbers of testicular cells of various types. When HT samples were treated with PRP, improvement was observed for all of these changes. This protective effect could be attributed to the growth factors present in PRP.

Conclusion: PRP appears to prevent the structural changes in the testes and the deterioration in sperm quality caused by CBZ-induced HT. This protective effect is likely due to mitigation of oxidative damage and elevation of testosterone levels.

目的:甲状腺功能减退症(HT)会影响精子生成,并与男性不育症有关。富血小板血浆(PRP)是一种富含生长因子的生物制品,可促进组织修复。本研究评估了富血小板血浆对卡比马唑(CBZ)诱导的 HT 中睾丸组织损伤的可能保护作用:方法:40 只雄性大鼠分为四组。方法:40 只雄性大鼠分为四组,分别口服 CBZ(1.35 毫克/千克,连续 45 天)诱导 HT。此外,还给予两剂 PRP(各 40 μL,分别于第 15 天和第 30 天在睾丸局部注射)。45 天后,从心脏抽取血液样本,测量三碘甲状腺原氨酸(T3)、甲状腺素(T4)和睾酮水平,并进行精液分析。为进行立体学评估,切除左侧睾丸,固定、包埋、切片,并用苏木精和伊红染色。切除右侧睾丸以评估抗氧化剂水平:结果:CBZ 可诱导 HT,其特征是 T3 和 T4 显著降低。HT与睾丸重量减少、精子参数受损、睾酮浓度降低、抗氧化活性减弱、睾丸成分体积减少以及各种类型的睾丸细胞总数减少有关。用 PRP 处理 HT 样本后,所有这些变化都得到了改善。这种保护作用可归因于 PRP 中的生长因子:PRP似乎可以防止CBZ诱导的HT引起的睾丸结构变化和精子质量下降。这种保护作用可能是由于减轻了氧化损伤和提高了睾酮水平。
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引用次数: 0
The effects of vitamin E supplementation on sperm parameters, chromatin integrity, and gene expression before and after freezing in aged mice. 补充维生素 E 对老年小鼠冷冻前后精子参数、染色质完整性和基因表达的影响。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-09-01 Epub Date: 2024-06-10 DOI: 10.5653/cerm.2023.06632
Sadegh Zarei, Farnoosh Molavi, Farzaneh Abbas Abasnezhad, Behanaz Majidi, Saeed Mohammadihosseinabad, Faezeh Esmaeili Ranjbar, Mahboubeh Vatanparast

Objective: Some age-related testicular changes, such as Sertoli cell vacuolization and blood-testis barrier breakdown, reduce total sperm production and male fertility. Therefore, this study investigated the effect of vitamin E on restoring testicular function in aged mice. Sperm cryo-resistance was also assessed.

Methods: Twenty-eight 48-week-old male Naval Medical Research Institute mice were divided into four groups for a daily gavage of vitamin E: the control group received distilled water, while the three treatment groups were administered 100, 200, and 400 mg/kg, respectively, for 4 weeks. Subsequently, semen analyses, DNA fragmentation index (DFI), and protamine deficiency tests were conducted. Testicular histology, tissue antioxidant enzyme activity, and gene expression levels were also assessed.

Results: The two higher dosages of vitamin E were associated with a higher sperm count, greater progressive motility, and improved sperm morphology (p<0.05). These benefits were also evident after sperm freezing (p<0.05). Although chromatin abnormalities increased following vitrification, the treatment groups showed better outcomes (p<0.05). The tubular diameter, epithelium height, and luminal diameters remained unchanged with age. The tissue antioxidant capacity was greater in the groups receiving the high doses of vitamin E. Additionally, significant increases in inhibitor of DNA binding protein-4 (Id4) and GDNF family receptor alpha-1 (Gfra1) expression were observed in the higher vitamin E dosage groups, and promyelocytic leukemia zinc finger protein (Plzf) expression was notably present in the 400 mg/kg treatment group compared to the control group (p<0.05).

Conclusion: Antioxidant supplementation might enhance reproductive outcomes in aging males. The observed effects included improved sperm cryo-resistance, which is advantageous for future applications such as sperm freezing or fertility preservation.

目的:一些与年龄有关的睾丸变化,如 Sertoli 细胞空泡化和血睾屏障破坏,会降低精子总产量和男性生育能力。因此,本研究探讨了维生素 E 对恢复老年小鼠睾丸功能的影响,并评估了精子的冷冻抗性。同时还评估了精子的抗冷冻能力:将 28 只 48 周大的雄性海军医学研究所小鼠分为四组,每天灌胃维生素 E:对照组服用蒸馏水,三个治疗组分别服用 100、200 和 400 毫克/千克,连续服用 4 周。随后进行精液分析、DNA碎片指数(DFI)和原胺缺乏试验。此外,还对睾丸组织学、组织抗氧化酶活性和基因表达水平进行了评估:结果:两种较高剂量的维生素 E 与较高的精子数量、较强的渐进运动性和精子形态的改善有关(p结论:补充抗氧化剂可能会提高老年男性的生殖能力。观察到的效果包括提高了精子的抗冷冻能力,这对未来精子冷冻或生育力保存等应用非常有利。
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引用次数: 0
Protective effects of Withania somnifera against cyclophosphamide-induced testicular damage in rats. 睡茄对环磷酰胺引起的大鼠睾丸损伤的保护作用
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-09-01 Epub Date: 2024-06-10 DOI: 10.5653/cerm.2023.06415
Mehrana Jafari, Ahmad Akbari, Zeynab Esmailpour, Zahra Nadi, Maryam Baazm

Objective: Cyclophosphamide (CP) is an alkylating agent commonly used in cancer treatment. It is known to have detrimental effects on the reproductive system, including the potential to cause infertility. Recently, herbal remedies have gained traction as a complementary approach to addressing these side effects. In this study, our goal was to investigate whether the aqueous-alcoholic extract of Withania somnifera (WS) could mitigate the adverse impacts of CP on testicular tissue.

Methods: Animals were randomly assigned to one of the following groups: control, WS (500 mg/kg), CP (100 mg/kg), CP+WS pre-treatment, and CP+WS post-treatment. WS was administered orally through gavage for 1 month. We assessed sperm parameters, testicular histopathology, and the expression of the Bax and Bcl2 genes in the experimental groups.

Results: Sperm parameters (including count, viability, and motility), the number of spermatogonia, the seminiferous tubule diameter, and Bcl2 gene expression, significantly decreased after CP injection (p<0.05). Conversely, the number of immotile sperm and Bax gene expression significantly increased (p<0.05). Treatment with WS, especially when administered as a pre-treatment, ameliorated the sperm parameters, histological alterations, and the expression of apoptosis-related genes (p<0.05).

Conclusion: The data suggest that WS may mitigate the detrimental effects of CP on testicular tissue by reducing apoptosis. Consequently, WS has the potential to be used as an adjunctive therapy to reduce the complications associated with CP treatment.

目的:环磷酰胺(CP)是一种常用于癌症治疗的烷化剂。众所周知,它对生殖系统有不利影响,包括可能导致不孕。最近,草药疗法作为解决这些副作用的辅助方法受到了关注。在这项研究中,我们的目标是调查睡茄(WS)的水醇提取物是否能减轻氯化石蜡对睾丸组织的不利影响:动物被随机分为以下几组:对照组、WS(500 毫克/千克)组、CP(100 毫克/千克)组、CP+WS 预处理组和 CP+WS 后处理组。WS通过灌胃口服给药,为期1个月。我们评估了实验组的精子参数、睾丸组织病理学以及 Bax 和 Bcl2 基因的表达:结果:注射 CP 后,精子参数(包括数量、存活率和活力)、精原细胞数量、曲细精管直径和 Bcl2 基因表达均显著下降(pConclusion):数据表明,WS 可通过减少细胞凋亡来减轻 CP 对睾丸组织的不利影响。因此,WS 有可能被用作一种辅助疗法,以减少与 CP 治疗相关的并发症。
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Clinical and Experimental Reproductive Medicine-CERM
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