Oral mucosal changes in tight junction proteins in patients with oral lichen planus.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Oral diseases Pub Date : 2024-10-01 Epub Date: 2024-03-04 DOI:10.1111/odi.14912

Xiaomeng Ren, Kaiyi Li, Xin Fang, Zhengda Zhu, Qianming Chen, Chunlei Li, Hong Hua

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Abstract

Objective: This study aimed to investigate the expression of tight junction, its distribution pattern in oral lichen planus samples and its potential association with the severity of oral lichen planus.

Materials and methods: Cross-sectional study designs were conducted. Transcriptome sequencing was conducted using oral mucosal tissues from 22 patients with oral lichen planus and 11 healthy controls. Immunohistochemistry and quantitative reverse transcription PCR were performed to verify the expression of claudin-1, claudin-4, occludin and zonula occludens-1 in oral mucosal tissues from another 30 patients with oral lichen planus and 26 healthy controls. The relationship between tight junction protein expression and oral lichen planus severity was explored using correlation analysis.

Results: 5603 and 2475 differentially expressed genes were upregulated and downregulated respectively, in oral lichen planus tissues. KEGG analysis showed that tight junctions including CLDN1, CLDN4, OCLN and TJP1 were downregulated in oral lichen planus. Claudin-1, claudin-4, occludin and zonula occludens-1 expression was verified to be significantly lower in oral lichen planus. Furthermore, correlation analyses showed that decreased occludin expression was positively related to oral lichen planus severity.

Conclusion: Decreased expression of TJ barrier proteins may be associated with the development of oral lichen planus.

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口腔扁平苔藓患者口腔黏膜紧密连接蛋白的变化。

研究目的本研究旨在调查口腔扁平苔藓样本中紧密结节的表达、分布模式及其与口腔扁平苔藓严重程度的潜在关联：采用横断面研究设计。使用 22 名口腔扁平苔藓患者和 11 名健康对照者的口腔黏膜组织进行转录组测序。对另外 30 名口腔扁平苔藓患者和 26 名健康对照者的口腔黏膜组织中 claudin-1、claudin-4、occludin 和 zonula occludens-1 的表达进行了免疫组化和定量反转录 PCR 检测。利用相关分析探讨了紧密连接蛋白表达与口腔扁平苔藓严重程度之间的关系：结果：在口腔扁平苔藓组织中，分别有 5603 个和 2475 个差异表达基因上调和下调。KEGG分析表明，口腔扁平苔藓中包括CLDN1、CLDN4、OCLN和TJP1在内的紧密连接基因被下调。Claudin-1、claudin-4、occludin和zonula occludens-1的表达在口腔扁平苔藓中显著降低。此外，相关分析表明，闭塞素表达的减少与口腔扁平苔藓的严重程度呈正相关：结论：TJ屏障蛋白表达的减少可能与口腔扁平苔藓的发生有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.