Reaction of Stylosanthes spp. ‘Campo Grande’ to Pratylenchus brachyurus and Meloidogyne javanica and analysis of the histopathology and histochemistry of their interactions

IF 2.1 4区农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Journal of Plant Diseases and Protection Pub Date : 2024-03-05 DOI:10.1007/s41348-024-00884-3

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Abstract

In an integrated nematode management system, the best results are generally obtained by rotating crops with resistant plants, antagonists, or poor host of parasites. Some reports indicate that Stylosanthes spp. have the potential to control nematodes. Thus, this study aimed to assess the reaction of Stylosanthes spp. ‘Campo Grande’ to Pratylenchus brachyurus and Meloidogyne javanica penetration and reproduction at different inoculum levels and examine the histopathology and histochemistry of parasitized plants. Stylosanthes did not prevent P. brachyurus penetration in roots. However, the number of penetrated nematodes was lower than that in soybean from 17 days after inoculation onwards. The numbers of second-stage and third-/fourth-stage juveniles of M. javanica in Stylosanthes roots were close to zero, and no females were observed. Assays conducted using increasing levels of inoculum (P. brachyurus or M. javanica) showed that Stylosanthes was resistant to the parasites, with a maximum reproduction factor of 0.59 for P. brachyurus and 0.07 for M. javanica. Histopathological analysis showed the presence of P. brachyurus in Stylosanthes, but without rupture of plant cells. M. javanica individuals were not observed. Histochemistry revealed the presence of phenolic compounds in the epidermis of Stylosanthes and proteins in plant cells. These results show that Stylosanthes spp. ‘Campo Grande’ can be used in crop rotation programs in fields with mixed infestation of P. brachyurus and M. javanica.

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大角耧斗菜'Campo Grande'对 brachyurus Pratylenchus 和 Meloidogyne javanica 的反应及其相互作用的组织病理学和组织化学分析

摘要在线虫综合管理系统中，一般通过轮作抗性植物、拮抗剂或寄生虫寄主不良的作物来获得最佳效果。一些报告指出，木贼属植物具有控制线虫的潜力。因此，本研究旨在评估'Campo Grande'木莲属植物在不同接种量下对Pratylenchus brachyurus和Meloidogyne javanica侵入和繁殖的反应，并检查寄生植物的组织病理学和组织化学。Stylosanthes 无法阻止 P. brachyurus 在根部的穿透。但是，从接种后 17 天开始，穿透的线虫数量低于大豆。在文竹根中，M. javanica 的第二阶段和第三/第四阶段幼虫数量接近零，而且没有观察到雌虫。使用不断增加的接种体（P. brachyurus 或 M. javanica）进行的试验表明，文殊兰对寄生虫具有抗性，P. brachyurus 的最大繁殖系数为 0.59，M. javanica 的最大繁殖系数为 0.07。组织病理学分析表明，在 Stylosanthes 中存在 P. brachyurus，但植物细胞没有破裂。没有观察到 M. javanica 个体。组织化学分析表明，木贼表皮中含有酚类化合物，植物细胞中含有蛋白质。这些结果表明，在有 P. brachyurus 和 M. javanica 混合侵扰的田地里，可以在轮作计划中使用 Stylosanthes spp.

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来源期刊

Journal of Plant Diseases and Protection 农林科学-农业综合

CiteScore

4.30

自引率

5.00%

发文量

124

审稿时长

3 months

期刊介绍： The Journal of Plant Diseases and Protection (JPDP) is an international scientific journal that publishes original research articles, reviews, short communications, position and opinion papers dealing with applied scientific aspects of plant pathology, plant health, plant protection and findings on newly occurring diseases and pests. "Special Issues" on coherent themes often arising from International Conferences are offered.