{"title":"Untargeted Metabolomics Reveals Altered Pathways in Phytoplasma-Infected Sesame Plants","authors":"","doi":"10.1007/s11105-024-01440-x","DOIUrl":null,"url":null,"abstract":"<h3>Abstract</h3> <p><em>Sesamum indicum</em> is an important oilseed crop rich in nutrients and antioxidants, cultivated worldwide owing to its economic value. Sesame fields are often challenged due to a disease caused by <em>Candidatus</em> Phytoplasma, a cell wall-lacking bacteria. Phytoplasma infection results in alteration in the plant’s physiological and metabolic responses. As metabolites are the final products that aid in a plant’s adaptation to several biotic and abiotic stresses, it is necessary to understand these metabolic changes. However, the changes at the metabolite level after Phytoplasma infection in sesame are poorly understood. To investigate the response of sesame plants to Phytoplasma infection, we analyzed the metabolic changes using liquid chromatography-tandem mass spectrometry (LC–MS/MS/MS). Data analysis led to the detection of 162 metabolites, among which 82 were upregulated, while 62 exhibited downregulating trends in the Phytoplasma-infected tissue. The upregulated metabolites include flavonoids, phenolics, lignans, glucosinolates, terpenoids, phytohormones, and some sugars, like trehalose. Phytoplasma infection also resulted in significant chlorophyll breakdown, suggesting impaired chloroplast physiology. We have also validated this LC–MS/MS data by biochemical analysis and analyzing the expression of a few genes behind the biosynthesis of the concerned metabolites using qRT-PCR analysis. The results indicate that plant metabolic networks undergo reprogramming upon Phytoplasma infection. Understanding this altered metabolic pathway may aid in developing a control measure for this disease.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11105-024-01440-x","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Sesamum indicum is an important oilseed crop rich in nutrients and antioxidants, cultivated worldwide owing to its economic value. Sesame fields are often challenged due to a disease caused by Candidatus Phytoplasma, a cell wall-lacking bacteria. Phytoplasma infection results in alteration in the plant’s physiological and metabolic responses. As metabolites are the final products that aid in a plant’s adaptation to several biotic and abiotic stresses, it is necessary to understand these metabolic changes. However, the changes at the metabolite level after Phytoplasma infection in sesame are poorly understood. To investigate the response of sesame plants to Phytoplasma infection, we analyzed the metabolic changes using liquid chromatography-tandem mass spectrometry (LC–MS/MS/MS). Data analysis led to the detection of 162 metabolites, among which 82 were upregulated, while 62 exhibited downregulating trends in the Phytoplasma-infected tissue. The upregulated metabolites include flavonoids, phenolics, lignans, glucosinolates, terpenoids, phytohormones, and some sugars, like trehalose. Phytoplasma infection also resulted in significant chlorophyll breakdown, suggesting impaired chloroplast physiology. We have also validated this LC–MS/MS data by biochemical analysis and analyzing the expression of a few genes behind the biosynthesis of the concerned metabolites using qRT-PCR analysis. The results indicate that plant metabolic networks undergo reprogramming upon Phytoplasma infection. Understanding this altered metabolic pathway may aid in developing a control measure for this disease.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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