Identification of Molecular Markers Associated with Genomic Regions Controlling Agronomic Traits in Bread Wheat Genotypes Under Different Moisture Conditions

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-09-05 DOI:10.1007/s11105-024-01494-x

Fatemeh Bavandpouri, Ezatollah Farshadfar, Kianoosh Cheghamirza, Mohsen Farshadfar, Mohammad Reza Bihamta, Amir Mohammad Mahdavi, Nadali Jelodar

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Abstract

The study of the association between polymorphism at the DNA level and the diversity of phenotypic traits is an essential tool in breeding programs. To identify informative microsatellite markers related to agronomic traits, this research including 25 bread wheat genotypes was carried out. The experiment was set up in a randomized complete block design with three replications in rainfed and irrigated conditions during two cropping seasons (2018–2020) in the cold Mediterranean climate of Iran. Variance analysis showed significant differences between genotypes for most of the traits. The 16 microsatellite primers out of 20 had considerable polymorphisms, and three markers, namely XCFD168-2D, XGWM350-7D, and XGWM136-1A, were introduced as the most significant markers for subsequent studies. Cluster analysis by the UPGMA method classified 25 wheat genotypes into four groups. Genotypes 1, 3, and 25 have the most significant genetic distance with genotypes 13, 7, and Pishgam. Association analysis by stepwise regression showed that in both years under rainfed conditions, the XGWM350 marker for 1000-grain weight, the XCFD5 marker for spike length, and the XGWM165 and XGWM70 markers for spike dry weight, and under irrigated conditions, the XGWM265 marker for grain yield exhibited significant associations. Also, the XGWM136 and XCFD5 were found to be common markers associated with agronomic traits for all the test environments. In addition, most of the markers were associated with 1000-grain weight, mitt penalty length, and spike grain weight in rainfed conditions and 1000-grain weight in irrigated conditions. After identifying molecular markers related to increased yield and drought tolerance, they can be used as selection criteria to accelerate wheat breeding programs. Also, these marker-trait associations can help wheat improvement programs through marker-assisted selection.

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不同水分条件下面包小麦基因型农艺性状控制基因组区域相关分子标记的鉴定

研究 DNA 水平的多态性与表型性状多样性之间的关联是育种计划中的一项重要工具。为了鉴定与农艺性状相关的信息微卫星标记，本研究包括了 25 个面包小麦基因型。实验采用随机完全区组设计，在伊朗寒冷地中海气候的两个种植季节（2018-2020 年），在雨水灌溉和灌溉条件下进行三次重复。变异分析表明，基因型之间在大多数性状上存在显著差异。20 个微卫星引物中的 16 个引物具有相当大的多态性，其中 XCFD168-2D、XGWM350-7D 和 XGWM136-1A 三个标记被作为最重要的标记引入后续研究。利用 UPGMA 方法进行聚类分析，将 25 个小麦基因型分为四组。基因型 1、3 和 25 与基因型 13、7 和 Pishgam 的遗传距离最大。逐步回归法的关联分析表明，在这两年的雨水灌溉条件下，千粒重的 XGWM350 标记、穗长的 XCFD5 标记、穗干重的 XGWM165 和 XGWM70 标记，以及在灌溉条件下，谷物产量的 XGWM265 标记都表现出显著的关联。在所有测试环境中，XGWM136 和 XCFD5 也是与农艺性状相关的常见标记。此外，大多数标记与雨水灌溉条件下的千粒重、穗刑长度和穗粒重以及灌溉条件下的千粒重有关。在确定了与增产和耐旱性相关的分子标记后，可将其作为加速小麦育种计划的选择标准。此外，这些标记与性状的关联还可以通过标记辅助选择帮助小麦改良计划。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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