Nonlinear polymer brush modified magnetic nanoparticles in phosphopeptides enrichment

IF 2.2 4区化学 Q3 CHEMISTRY, PHYSICAL Colloid and Polymer Science Pub Date : 2024-03-04 DOI:10.1007/s00396-024-05244-z

Zahra Shirzadi, Habibollah Baharvand, Mahshid Nikpour Nezhati, Homayon Ahmad Panahi, Reza H. Sajedi

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Abstract

Magnetic nanoparticles attached to hydrophilic polymer brushes containing immobilized Fe³⁺ ions were synthesized and used as a new strategy for phosphopeptide enrichment from digested tryptic proteins. To test the performance of nanoparticles in phosphopeptide enrichment, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used. Magnetic nanoparticles with the following features such as high adsorption capacity (about 360 mg/g), easy control and transfer by external magnetic field (high magnetic saturation, about 50 emu/g), excellent phosphopeptide recovery (93.4%) and no shadow effect (due to their non-porous structure), were synthesized. It has been shown that the presence of other proteins in high concentrations does not affect on the performance of nanoparticles. Furthermore, the lowest concentration of digested β-casein phosphopeptides detectable by nanoparticles was determined to be approximately 0.5 fmol µL^− 1. The function of synthesized nanoparticles to enrich phosphopeptides in complex biological samples was also demonstrated by isolating four endogenous phosphopeptides from human serum.

Graphical Abstract

A dispersion containing Fe-MNPs is added to a phosphopeptide solution. Then, using a magnet, particles containing phosphopeptides are separated from the solution

Abstract Image

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非线性聚合物刷修饰磁性纳米粒子在磷酸肽富集中的应用

研究人员合成了附着在含有固定化 Fe3+ 离子的亲水性聚合物刷上的磁性纳米粒子，并将其作为一种从消化胰蛋白酶蛋白中富集磷酸肽的新策略。为了测试纳米颗粒在富集磷酸肽方面的性能，使用了基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）。合成的磁性纳米粒子具有以下特点：吸附容量高（约 360 mg/g）、易受外磁场控制和转移（磁饱和度高，约 50 emu/g）、磷肽回收率高（93.4%）、无阴影效应（因其无孔结构）。研究表明，高浓度的其他蛋白质不会影响纳米粒子的性能。此外，经测定，纳米颗粒可检测到的最低消化β-酪蛋白磷酸肽浓度约为 0.5 fmol µL-1。通过从人血清中分离出四种内源性磷酸肽，也证明了合成纳米粒子在复杂生物样品中富集磷酸肽的功能。

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来源期刊

Colloid and Polymer Science 化学-高分子科学

CiteScore

4.60

自引率

4.20%

发文量

111

审稿时长

2.2 months

期刊介绍： Colloid and Polymer Science - a leading international journal of longstanding tradition - is devoted to colloid and polymer science and its interdisciplinary interactions. As such, it responds to a demand which has lost none of its actuality as revealed in the trends of contemporary materials science.