Methylation Status of miR-34a and miR-126 in Non-Small Cell Lung Cancer (NSCLC) Tumor Tissues.

Q2 Biochemistry, Genetics and Molecular Biology Iranian Biomedical Journal Pub Date : 2024-01-01 Epub Date: 2023-10-14 DOI:10.61186/ibj.3845

Nazanin Mehrzad, Mohammad Saber Zamani, Amirabbas Rahimi, Masoud Shamaei, Morteza Karimipoor

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Abstract

Background: MiR-34a and miR-126 mainly act as tumor suppressors and are often downregulated in various cancers, including non-small cell lung cancer (NSCLC). We aimed to determine the methylation status of miR-34a and miR-126 in NSCLC patients.

Methods: The current study included 63 paraffin-embedded NSCLC and paired adjacent normal tissues. After DNA extraction and bisulfite treatment, the methylation status of miR-34a and miR-126 were evaluated using the MSP method.

Results: There was no statistically significant difference between tumor and normal tissues regarding the methylation status of miR-34a and miR-126 (p > 0.05). Moreover, we found no significant correlation between the methylation status of miR-34a and miR-126 with patients’ demographic parameters, including gender, age, and pathology subtype (p > 0.05).

Conclusion: Considering the low expression of mir-126 and mir-34 in NSCLC, more sensitive methods are recommended to be exploited for detecting the level of methylation or underlying mechanisms other than promoter hypermethylation in silencing these genes in NSCLC.

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非小细胞肺癌（NSCLC）肿瘤组织中 miR-34a 和 miR-126 的甲基化状态

背景：非小细胞肺癌占肺部肿瘤的85%，是全球癌症死亡的主要原因。MiR-34a和miR-126主要作为肿瘤抑制因子，在包括非小细胞肺癌在内的各种癌症中经常下调：本研究包括 63 例石蜡包埋的 NSCLC 和配对的邻近正常组织。方法：本研究纳入了 63 例石蜡包埋的 NSCLC 和配对的邻近正常组织，在提取 DNA 并进行亚硫酸氢盐处理后，采用 MSP 方法评估了 miR-34a 和 miR-126 的甲基化状态：结果：miR-34a和miR-126的甲基化状态在肿瘤组织和正常组织之间没有统计学差异（P > 0.05）。此外，我们还发现 miR-34a 和 miR-126 的甲基化状态与患者的性别、年龄和病理亚型等人口统计学参数无明显相关性（P > 0.05）：考虑到mir-126和mir-34在NSCLC中的低表达，建议采用更灵敏的方法检测甲基化水平或NSCLC中沉默这些基因的启动子高甲基化以外的潜在机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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