Polyelectrolyte Complexes and Coacervates Formed by De novo-Designed Peptides and Oligonucleotide

IF 4.1 2区化学 Q2 POLYMER SCIENCE Chinese Journal of Polymer Science Pub Date : 2024-03-04 DOI:10.1007/s10118-024-3096-6

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Abstract

The liquid-liquid phase separation of biopolymers in living cells contains multiple interactions and occurs in a dynamic environment. Resolving the regulation mechanism is still a challenge. In this work, we designed a series of peptides (XXLY)₆SSSGSS and studied their complexation and coacervation behavior with single-stranded oligonucleotides. The “X” and “Y” are varied to combine known amounts of charged and non-charged amino acids, together with the introduction of secondary structures and pH responsiveness. Results show that the electrostatic interaction, which is described as charge density, controls both the strength of complexation and the degree of chain relaxation, and thus determines the growth and size of the coacervates. The hydrophobic interaction is prominent when the charges are neutralized. Interestingly, the secondary structures of peptides exhibit profound effect on the morphology of the phases, such as solid phase to liquid phase transition. Our study gains insight into the phase separation under physiological conditions. It is also helpful to create coacervates with desirable structures and functions.

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新设计的多肽和寡核苷酸形成的聚电解质复合物和共液态化合物

摘要活细胞中生物聚合物的液-液相分离包含多种相互作用，并发生在动态环境中。解决其调控机制仍是一项挑战。在这项工作中，我们设计了一系列多肽 (XXLY)6SSSSGSS，并研究了它们与单链寡核苷酸的复合和共轭行为。通过改变 "X "和 "Y "来组合已知数量的带电和不带电氨基酸，同时引入二级结构和 pH 响应性。结果表明，静电作用（用电荷密度来描述）控制着复合的强度和链的松弛程度，从而决定了凝聚体的生长和大小。当电荷中和时，疏水相互作用就会凸显出来。有趣的是，肽的二级结构对相的形态（如固相到液相的转变）有着深远的影响。我们的研究有助于深入了解生理条件下的相分离现象。这也有助于创造出具有理想结构和功能的凝聚态物质。

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来源期刊

Chinese Journal of Polymer Science 化学-高分子科学

CiteScore

7.10

自引率

11.60%

发文量

218

审稿时长

6.0 months

期刊介绍： Chinese Journal of Polymer Science (CJPS) is a monthly journal published in English and sponsored by the Chinese Chemical Society and the Institute of Chemistry, Chinese Academy of Sciences. CJPS is edited by a distinguished Editorial Board headed by Professor Qi-Feng Zhou and supported by an International Advisory Board in which many famous active polymer scientists all over the world are included. The journal was first published in 1983 under the title Polymer Communications and has the current name since 1985. CJPS is a peer-reviewed journal dedicated to the timely publication of original research ideas and results in the field of polymer science. The issues may carry regular papers, rapid communications and notes as well as feature articles. As a leading polymer journal in China published in English, CJPS reflects the new achievements obtained in various laboratories of China, CJPS also includes papers submitted by scientists of different countries and regions outside of China, reflecting the international nature of the journal.