Mitofusin-2 enhances cervical cancer progression through Wnt/β-catenin signaling.

IF 2.9 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY BMB Reports Pub Date : 2024-04-01

Sung Yong Ahn

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Abstract

Overexpression of mitofusin-2 (MFN2), a mitochondrial fusion protein, is frequently associated with poor prognosis in cervical cancer patients. Here, I aimed to investigate the involvement of MFN2 in cervical cancer progression and determine the effect of MFN2 on prognosis in cervical cancer patients. After generating MFN2-knockdown SiHa cells derived from squamous cell carcinoma, I investigated the effect of MFN2 on SiHa cell proliferation using the Cell Counting Kit-8 assay and determined the mRNA levels of proliferation markers. Colony-forming ability and tumorigenesis were evaluated using a colonyformation assay and tumor xenograft mouse models. The migratory and invasive abilities associated with MFN2 were measured using wound-healing and invasion assays. Wnt/β-cateninmediated epithelial-mesenchymal transition (EMT) markers related to MFN2 were assessed through quantitative RT-PCR. MFN2-knockdown SiHa cells exhibited reduced proliferation, colony formation, migration, invasion, and tumor formation in vivo. The motility of SiHa cells with MFN2 knockdown was reduced through Wnt/β-catenin-mediated EMT inhibition. MFN2 promoted cancer progression and tumorigenesis in SiHa cells. Overall, MFN2 could serve as a therapeutic target and a novel biomarker for cervical cancer. [BMB Reports 2024; 57(4): 194-199].

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Mitofusin-2 通过 Wnt/β-catenin 信号增强宫颈癌的进展。

线粒体融合蛋白Mitofusin-2（MFN2）的过表达常常与宫颈癌患者的不良预后有关。在此，笔者旨在研究 MFN2 参与宫颈癌进展的情况，并确定 MFN2 对宫颈癌患者预后的影响。在制备了MFN2-敲除的鳞状细胞癌SiHa细胞后，我使用细胞计数试剂盒-8检测法研究了MFN2对SiHa细胞增殖的影响，并测定了增殖标志物的mRNA水平。利用集落形成试验和肿瘤异种移植小鼠模型对集落形成能力和肿瘤发生进行了评估。使用伤口愈合和侵袭试验测量了与 MFN2 相关的迁移和侵袭能力。通过定量 RT-PCR 评估了与 MFN2 相关的 Wnt/β-catenin 介导的上皮-间质转化（EMT）标记物。MFN2-敲除的SiHa细胞在体内表现出增殖、集落形成、迁移、侵袭和肿瘤形成的减少。通过Wnt/β-catenin介导的EMT抑制作用，MFN2敲除的SiHa细胞的运动能力降低。MFN2促进了SiHa细胞的癌症进展和肿瘤发生。总之，MFN2可作为宫颈癌的治疗靶点和新型生物标记物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMB Reports 生物-生化与分子生物学

CiteScore

5.10

自引率

7.90%

发文量

141

审稿时长

1 months

期刊介绍： The BMB Reports (BMB Rep, established in 1968) is published at the end of every month by Korean Society for Biochemistry and Molecular Biology. Copyright is reserved by the Society. The journal publishes short articles and mini reviews. We expect that the BMB Reports will deliver the new scientific findings and knowledge to our readers in fast and timely manner.