Prevalence of salivary microbial load and lactic acid presence in diabetic and non-diabetic individuals with different dental caries stages.

Restorative Dentistry & Endodontics Pub Date : 2024-01-12 eCollection Date: 2024-02-01 DOI:10.5395/rde.2024.49.e4
Monika Mohanty, Shashirekha Govind, Shakti Rath
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Abstract

Objectives: This study aims to correlate caries-causing microorganism load, lactic acid estimation, and blood groups to high caries risk in diabetic and non-diabetic individuals and low caries risk in healthy individuals.

Materials and methods: This study includes 30 participants divided into 3 groups: Group A, High-risk caries diabetic individuals; Group B, High-risk caries non-diabetic individuals; and Group C, Low-risk caries individuals. The medical condition, oral hygiene, and caries risk assessment (American Dental Association classification and International Caries Detection and Assessment System scoring) were documented. Each individual's 3 mL of saliva was analyzed for microbial load and lactic acid as follows: Part I: 2 mL for microbial quantity estimation using nutrient agar and blood agar medium, biochemical investigation, and carbohydrate fermentation tests; Part II: 0.5 mL for lactic acid estimation using spectrophotometric analysis. Among the selected individuals, blood group correlation was assessed. The χ2 test, Kruskal-Wallis test, and post hoc analysis were done using Dunn's test (p < 0.05).

Results: Group A had the highest microbial load and lactic acid concentration, followed by Groups B and C. The predominant bacteria were Lactobacilli (63.00 ± 15.49) and Streptococcus mutans (76.00 ± 13.90) in saliva. Blood Group B is prevalent in diabetic and non-diabetic high-risk caries patients but statistically insignificant.

Conclusions: Diabetic individuals are more susceptible to dental caries due to high microbial loads and increased lactic acid production. These factors also lower the executing tendency of neutrophils, which accelerates microbial accumulation and increases the risk of caries in diabetic individuals.

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不同龋齿阶段的糖尿病患者和非糖尿病患者的唾液微生物量和乳酸含量。
研究目的:本研究旨在将糖尿病患者和非糖尿病患者的致龋微生物负荷、乳酸估计值和血型与高龋风险以及健康人的低龋风险相关联:本研究将 30 名参与者分为 3 组:A 组,龋齿风险高的糖尿病患者;B 组,龋齿风险高的非糖尿病患者;C 组,龋齿风险低的患者。每个人的身体状况、口腔卫生和龋病风险评估(美国牙科协会分类和国际龋病检测与评估系统评分)均已记录在案。对每个人的 3 毫升唾液进行微生物负荷和乳酸分析,具体方法如下:第一部分:2 毫升用于使用营养琼脂和血琼脂培养基估算微生物数量、生化调查和碳水化合物发酵测试;第二部分:0.5 毫升用于使用分光光度法估算乳酸。对所选个体的血型相关性进行了评估。采用χ2检验、Kruskal-Wallis检验和邓恩检验(P<0.05)进行事后分析:唾液中最主要的细菌是乳酸杆菌(63.00±15.49)和变异链球菌(76.00±13.90)。血型 B 在糖尿病和非糖尿病高危龋齿患者中普遍存在,但在统计学上并不显著:结论:由于微生物负荷高和乳酸分泌增加,糖尿病患者更容易患龋齿。这些因素也降低了中性粒细胞的执行倾向,从而加速了微生物的积累,增加了糖尿病患者患龋齿的风险。
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CiteScore
0.20
自引率
0.00%
发文量
35
审稿时长
12 weeks
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