WITHDRAWN: PIRES2-EGFP/CTB-UreI vaccination activated a mixed Th1/Th2/Th17 immune system defense towards Helicobacter pylori infection in the BALB/c mice model.

Sana Ghasemifar, Omid Chabak, Tohid Piri-Gharaghie, Abbas Doosti
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Abstract

This article has been withdrawn at the request of the Editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/policies/article-withdrawal. The data presented in the manuscript was deemed severely flawed after appearing online as an Article in Press. The scientific community raised concerns about the methodology (including but not limited to major technical issues) used in the study and the subsequent conclusions drawn from the presented experiments. After careful investigation, the Vaccine editorial office concluded that the data in the publication was indeed severely flawed and that the concerns raised by the scientific community were valid. Therefore, the journal editors decided to withdraw the article and sincerely apologize for any inconvenience caused.

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在 BALB/c 小鼠模型中,接种 PIRES2-EGFP/CTB-UreI 疫苗可激活 Th1/Th2/Th17 混合免疫系统防御幽门螺旋杆菌感染。
人类胃炎、胃溃疡、远端胃癌和胃黏膜淋巴瘤的发生与幽门螺旋杆菌(H. pylori)密切相关。由于抗生素耐药性日益普遍,接种疫苗是一种有效的预防措施。融合疫苗接种是一种潜在的实用方法。本研究将霍乱毒素 B 亚基(CTB)与抗原性幽门螺杆菌尿素酶 I 亚基(CTB-UreI)结合,制成了一种融合疫苗。CTB-UreI DNA疫苗被化学克隆到pIRES2-EGFP中,并通过PCR和限制性酶消化验证了克隆的成功。对 CTB-UreI 在大肠杆菌 BL21(DE3) 中的诱导进行了研究。在 BALB/c 小鼠中评估了疫苗的免疫原性和免疫保护效力。Western 印迹检测成功确定了 CTB-UreI 的活化。相比之下,接种 pIRES2-EGFP/CTB-UreI 疫苗的 BALB/c 小鼠血液样本中的 IgG、IgA、IFN-γ、IL-4 和 IL-17 水平更高。此外,胃损伤和细菌负荷也有所减少。此外,接种了 pIRES2-EGFP/CTB-UreI 的 BALB/c 小鼠对幽门螺杆菌的挑战表现出了高水平的免疫力(100%)。pIRES2-EGFP/CTB-UreI引起了Th1/Th2/Th17组合免疫反应,可能有助于形成有效的防御机制。我们的数据表明,使用这种融合疫苗预防幽门螺杆菌感染是一种很有前景的选择。
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