A normalized parameter for comparison of biofilm dispersants in vitro

IF 5.9 Q1 MICROBIOLOGY Biofilm Pub Date : 2024-03-03 DOI:10.1016/j.bioflm.2024.100188
Shuang Tian , Linqi Shi , Yijin Ren , Henny C. van der Mei , Henk J. Busscher
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Abstract

Dispersal of infectious biofilms increases bacterial concentrations in blood. To prevent sepsis, the strength of a dispersant should be limited to allow the immune system to remove dispersed bacteria from blood, preferably without antibiotic administration. Biofilm bacteria are held together by extracellular polymeric substances that can be degraded by dispersants. Currently, comparison of the strength of dispersants is not possible by lack of a suitable comparison parameter. Here, a biofilm dispersal parameter is proposed that accounts for differences in initial biofilm properties, dispersant concentration and exposure time by using PBS as a control and normalizing outcomes with respect to concentration and time. The parameter yielded near-identical values based on dispersant-induced reductions in biomass or biofilm colony-forming-units and appeared strain-dependent across pathogens. The parameter as proposed is largely independent of experimental methods and conditions and suitable for comparing different dispersants with respect to different causative strains in particular types of infection.

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用于比较体外生物膜分散剂的标准化参数
传染性生物膜的分散会增加血液中的细菌浓度。为预防败血症,分散剂的强度应受到限制,以便免疫系统能够清除血液中分散的细菌,最好不使用抗生素。生物膜细菌由细胞外聚合物物质固定在一起,分散剂可将其降解。目前,由于缺乏合适的比较参数,无法对分散剂的强度进行比较。在此,我们提出了一种生物膜分散参数,该参数考虑了生物膜初始特性、分散剂浓度和暴露时间的差异,以 PBS 作为对照,并对浓度和时间进行归一化处理。根据分散剂诱导的生物量或生物膜集落形成单位的减少,该参数产生了几乎相同的值,并且在不同病原体中似乎与菌株有关。所提出的参数在很大程度上与实验方法和条件无关,适用于在特定类型的感染中比较不同分散剂对不同致病菌株的影响。
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来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
期刊介绍:
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