Synthesis of thiol-containing DNA-oligonucleotides using a phosphoramidite reagent based on trans -4-hydroxy-L-prolinol

Y. Lamekina, T. Seviarynchyk, E. Ulashchik, T. P. Akhlamionok, P. Y. Baryshchyk, B. Ranishenka, V. V. Shmanai
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Abstract

In this work, a new phosphoramidite reagent for the preparation of thiol-modified oligonucleotides was synthesized. Thiol-specific reagents have been used to demonstrate the reactivity of thiol groups. After modification, conjugates were purified via gel-filtration and characterized with HPLC-MS.
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使用基于反式-4-羟基-L-脯氨醇的亚磷酰胺试剂合成含硫醇的 DNA-寡核苷酸
在这项工作中,合成了一种用于制备硫醇修饰寡核苷酸的新型亚磷酰胺试剂。硫醇特异性试剂用于证明硫醇基团的反应性。修饰后的共轭物经凝胶过滤纯化,并用 HPLC-MS 进行表征。
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0.30
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0.00%
发文量
38
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