Cloning and functional analysis of ZmMADS42 gene in maize.

IF 4.5 2区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2024-12-31 Epub Date: 2024-03-11 DOI:10.1080/21645698.2024.2328384

Yang Zhao, Jianyu Lu, Bo Hu, Peng Jiao, Bai Gao, Zhenzhong Jiang, Siyan Liu, Shuyan Guan, Yiyong Ma

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Abstract

Maize (Zea mays L.) is the most important cereal crop in the world. Flowering period and photoperiod play important roles in the reproductive development of maize. This study, investigated ZmMADS42, a gene that is highly expressed in the shoot apical meristem. Agrobacterium infection was used to successfully obtain overexpressed ZmMADS42 plants. Fluorescence quantitative PCR revealed that the expression of the ZmMADS42 gene in the shoot apical meristem of transgenic plants was 2.8 times higher than that of the wild-type(WT). In addition, the expression of the ZmMADS42 gene in the endosperm was 2.4 times higher than that in the wild-type. The seed width of the T2 generation increased by 5.35%, whereas the seed length decreased by 7.78% compared with that of the wild-type. Dissection of the shoot tips of transgenic and wild-type plants from the 7-leaf stage to the 9-leaf stage revealed that the transgenic plants entered the differentiation stage earlier and exhibited more tassel meristems during their vegetative growth period. The mature transgenic plants were approximately 20 cm shorter in height and had a lower panicle position than the wild-type plants. Comparing the flowering period, the tasseling, powdering, and silking stages of the transgenic plants occurred 10 days earlier than those of the wild-type plants. The results showed that the ZmMADS42 gene played a significant role in regulating the flowering period and plant height of maize.

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玉米 ZmMADS42 基因的克隆和功能分析。

玉米（Zea mays L.）是世界上最重要的谷类作物。花期和光周期对玉米的生殖发育起着重要作用。本研究对 ZmMADS42 进行了研究，这是一个在芽顶端分生组织中高表达的基因。利用农杆菌感染成功获得了过表达 ZmMADS42 的植株。荧光定量 PCR 结果显示，转基因植株芽尖分生组织中 ZmMADS42 基因的表达量是野生型（WT）的 2.8 倍。此外，ZmMADS42 基因在胚乳中的表达量是野生型的 2.4 倍。与野生型相比，T2 代的种子宽度增加了 5.35%，而种子长度则减少了 7.78%。对转基因植株和野生型植株从 7 叶期到 9 叶期的芽尖进行解剖发现，转基因植株较早进入分化期，在无性生长期表现出更多的抽穗分生组织。成熟的转基因植株高度比野生型植株矮约 20 厘米，圆锥花序位置也比野生型植株低。比较花期，转基因植株的抽穗期、粉期和抽丝期比野生型植株早 10 天。结果表明，ZmMADS42 基因在调控玉米的花期和株高方面发挥了重要作用。

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来源期刊

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

8.10

自引率

10.30%

发文量

期刊介绍： GM Crops & Food - Biotechnology in Agriculture and the Food Chain aims to publish high quality research papers, reviews, and commentaries on a wide range of topics involving genetically modified (GM) crops in agriculture and genetically modified food. The journal provides a platform for research papers addressing fundamental questions in the development, testing, and application of transgenic crops. The journal further covers topics relating to socio-economic issues, commercialization, trade and societal issues. GM Crops & Food aims to provide an international forum on all issues related to GM crops, especially toward meaningful communication between scientists and policy-makers. GM Crops & Food will publish relevant and high-impact original research with a special focus on novelty-driven studies with the potential for application. The journal also publishes authoritative review articles on current research and policy initiatives, and commentary on broad perspectives regarding genetically modified crops. The journal serves a wide readership including scientists, breeders, and policy-makers, as well as a wider community of readers (educators, policy makers, scholars, science writers and students) interested in agriculture, medicine, biotechnology, investment, and technology transfer. Topics covered include, but are not limited to: • Production and analysis of transgenic crops • Gene insertion studies • Gene silencing • Factors affecting gene expression • Post-translational analysis • Molecular farming • Field trial analysis • Commercialization of modified crops • Safety and regulatory affairs BIOLOGICAL SCIENCE AND TECHNOLOGY • Biofuels • Data from field trials • Development of transformation technology • Elimination of pollutants (Bioremediation) • Gene silencing mechanisms • Genome Editing • Herbicide resistance • Molecular farming • Pest resistance • Plant reproduction (e.g., male sterility, hybrid breeding, apomixis) • Plants with altered composition • Tolerance to abiotic stress • Transgenesis in agriculture • Biofortification and nutrients improvement • Genomic, proteomic and bioinformatics methods used for developing GM cops ECONOMIC, POLITICAL AND SOCIAL ISSUES • Commercialization • Consumer attitudes • International bodies • National and local government policies • Public perception, intellectual property, education, (bio)ethical issues • Regulation, environmental impact and containment • Socio-economic impact • Food safety and security • Risk assessments