M. Yu. Gallyamova, K. N. Vagin, N. M. Vasilevsky, N. I. Hammadov
{"title":"Analysis of Genome Variability of Escherichia coli When Exposed to Ionizing Radiation","authors":"M. Yu. Gallyamova, K. N. Vagin, N. M. Vasilevsky, N. I. Hammadov","doi":"10.1134/s1022795424010058","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The study of changes in the genetic apparatus of biological systems due to the influence of physical factors contributes to understanding the mechanisms of adaptation. The article is devoted to the analysis of the genome of the modified <i>Escherichia coli</i> PL-6 variant obtained as a result of repeated and gradually increasing exposure to <sup>60</sup>Co γ rays at the Researcher facility. The integrity of the genetic material of the studied bacterial cells was checked by electrophoresis in 1.7% agarose gel. For the analysis of genome modifications, primers were designed to amplify several loci characterized by homology in a variety of <i>E. coli</i> strains. On the basis of the indicators of the number and size of amplified products using each of the presented primer combinations in <i>E. coli</i> before and after gamma irradiation, a significant change in the genome was established.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"36 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2024-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Journal of Genetics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s1022795424010058","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0
Abstract
The study of changes in the genetic apparatus of biological systems due to the influence of physical factors contributes to understanding the mechanisms of adaptation. The article is devoted to the analysis of the genome of the modified Escherichia coli PL-6 variant obtained as a result of repeated and gradually increasing exposure to 60Co γ rays at the Researcher facility. The integrity of the genetic material of the studied bacterial cells was checked by electrophoresis in 1.7% agarose gel. For the analysis of genome modifications, primers were designed to amplify several loci characterized by homology in a variety of E. coli strains. On the basis of the indicators of the number and size of amplified products using each of the presented primer combinations in E. coli before and after gamma irradiation, a significant change in the genome was established.
期刊介绍：
Russian Journal of Genetics is a journal intended to make significant contribution to the development of genetics. The journal publishes reviews and experimental papers in the areas of theoretical and applied genetics. It presents fundamental research on genetic processes at molecular, cell, organism, and population levels, including problems of the conservation and rational management of genetic resources and the functional genomics, evolutionary genomics and medical genetics.
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