Genome-Wide Identification and Analysis of the CCoAOMT Gene Family Revealed Its Relationship to Pomegranate Seed Hardness

IF 0.5 4区生物学 Q4 GENETICS & HEREDITY Russian Journal of Genetics Pub Date : 2024-03-11 DOI:10.1134/s1022795424020121

H. M. Suo, H. H. Ni, L. Hu, F. Y. Yuan, M. W. Zhang, S. M. Zhang

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引用次数: 0

Abstract

Caffeoyl coenzyme A-O-methyltransferase (CCoAOMT) has a critical function in the lignin biosynthesis pathway. However, its role in pomegranate is not clear. In this research, we identified 11 members of the CCoAOMT gene family based on pomegranate genome-wide data. The 11 CCoAOMT genes were distributed non-uniformly on chromosomes 2, 3 and 4 of pomegranate. Phylogenetic analysis showed that pomegranate CCoAOMT9 and Camellia sinensis CCoAOMT genes clustered into one branch. Promoter cis-acting progenitor analysis revealed a role for the pomegranate CCoAOMT gene in the ABA/MeJA response. The expression of CCoAOMT8, CCoAOMT9 and CCoAOMT10 was higher in hard-seeded cultivars (‘Hongyushizi’ and ‘Baiyushizi’) than in soft-seeded cultivar (Tunisia) as detected and analyzed by q-PCR. The results suggested that CCoAOMT8, CCoAOMT9, and CCoAOMT10 might be related to lignin biosynthesis. The findings of this work provided a basis for further investigation of the role of the pomegranate CCoAOMT gene in lignin biosynthesis.

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CCoAOMT 基因家族的全基因组鉴定和分析揭示了其与石榴籽硬度的关系

摘要咖啡酰辅酶 A-O-甲基转移酶（CCoAOMT）在木质素生物合成途径中具有重要功能。然而，它在石榴中的作用尚不明确。在这项研究中，我们根据石榴全基因组数据确定了 11 个 CCoAOMT 基因家族成员。这 11 个 CCoAOMT 基因非均匀地分布在石榴的 2、3 和 4 号染色体上。系统进化分析表明，石榴 CCoAOMT9 基因和山茶 CCoAOMT 基因聚为一个分支。启动子顺式作用原基分析表明，石榴 CCoAOMT 基因在 ABA/MeJA 反应中发挥作用。通过 q-PCR 检测和分析，CCoAOMT8、CCoAOMT9 和 CCoAOMT10 在硬籽栽培品种（'红石榴'和'白玉石榴'）中的表达量高于软籽栽培品种（突尼斯）。结果表明，CCoAOMT8、CCoAOMT9 和 CCoAOMT10 可能与木质素的生物合成有关。这项工作的发现为进一步研究石榴 CCoAOMT 基因在木质素生物合成中的作用提供了依据。

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来源期刊

Russian Journal of Genetics 生物-遗传学

CiteScore

1.00

自引率

33.30%

发文量

126

审稿时长

1 months

期刊介绍： Russian Journal of Genetics is a journal intended to make significant contribution to the development of genetics. The journal publishes reviews and experimental papers in the areas of theoretical and applied genetics. It presents fundamental research on genetic processes at molecular, cell, organism, and population levels, including problems of the conservation and rational management of genetic resources and the functional genomics, evolutionary genomics and medical genetics.