The combined application of rutin and silicon alleviates osmotic stress in maize seedlings by triggering accumulation of osmolytes and antioxidants’ defense mechanisms

IF 3.4 3区生物学 Q1 PLANT SCIENCES Physiology and Molecular Biology of Plants Pub Date : 2024-03-09 DOI:10.1007/s12298-024-01430-z

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Abstract

Silicon (Si) has been shown to improve plant defenses against a variety of stresses. However, how rutin (Rut) affects stress factors is yet to be fully explored. Moreover, their combined role in osmotic stress response remains unclear. The current study was performed to determine how the use of Rut and Si, both separately and in combination, improved the physiological resilience of maize seedlings to two levels of osmotic stress (induced by polyethylene glycol (PEG) 6000). We aimed to enhance osmotic stress tolerance with the simultaneous use of Rut and Si. First, we selected the best water status and the lowest membrane damage enhancing concentration of Rut (60 ppm) and Si (1 mM) to research their tolerance and resistance to osmotic stress (moderate: 10% PEG, severe: 15% PEG). The application of Rut and Si separately and together reduced oxidative stress by decreasing the reactive oxygen species and improved the relative water content, osmoprotectants (proline, total soluble sugar, and glycine-betaine), ascorbate level, and some antioxidant defense-related enzyme activities and their gene expression in maize seedlings under osmotic stress. However, these effects were more promising under moderate stress. As a result, findings from the study indicate the synergistic effect of combined Rut and Si on osmotic stress tolerance in maize seedlings. Overall, the combination of Rut and Si was more effective than independent Rut and Si in reducing osmotic stress in maize seedlings. Here, it was clear that Rut played an active role in alleviating stress. This combined application can be useful for developing drought tolerance in crops for the agriculture sector.

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芦丁和硅的联合应用通过激发渗透溶质的积累和抗氧化剂的防御机制缓解玉米幼苗的渗透胁迫

摘要硅（Si）已被证明可提高植物对各种胁迫的防御能力。然而，芦丁（Rutin）如何影响胁迫因子还有待充分探讨。此外，它们在渗透胁迫响应中的联合作用仍不清楚。本研究旨在确定芦丁和硅的单独或联合使用如何改善玉米幼苗对两种水平渗透胁迫（由聚乙二醇（PEG）6000 诱导）的生理恢复能力。我们的目标是通过同时使用 Rut 和 Si 来提高渗透胁迫耐受性。首先，我们选择了最佳水分状态和最低膜损伤增强浓度的 Rut（60 ppm）和 Si（1 mM），以研究它们对渗透胁迫的耐受性和抗性（中度：10% PEG，重度：15% PEG）。单独或同时施用 Rut 和 Si 可通过减少活性氧来降低氧化应激，并能提高渗透胁迫下玉米幼苗的相对含水量、渗透保护剂（脯氨酸、总可溶性糖和甘氨酸-甜菜碱）、抗坏血酸水平和一些抗氧化防御相关酶的活性及其基因表达。不过，在中度胁迫下，这些影响更有希望。因此，研究结果表明，Rut 和 Si 对玉米幼苗的渗透胁迫耐受性有协同作用。总体而言，在降低玉米幼苗的渗透胁迫方面，Rut 和 Si 的组合比独立的 Rut 和 Si 更有效。由此可见，Rut 在缓解胁迫方面发挥了积极作用。这种组合应用可用于开发农业作物的抗旱能力。

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来源期刊

Physiology and Molecular Biology of Plants PLANT SCIENCES-

CiteScore

7.10

自引率

0.00%

发文量

126

期刊介绍： Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.