Towards Sustainable Pest Management: Toxicity, Biochemical Effects, and Molecular Docking Analysis of Ocimum basilicum (Lamiaceae) Essential Oil on Agrotis ipsilon and Spodoptera littoralis (Lepidoptera: Noctuidae)

IF 1.4 3区农林科学 Q2 ENTOMOLOGY Neotropical Entomology Pub Date : 2024-03-13 DOI:10.1007/s13744-024-01137-6

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Abstract

Over the last decade, essential oils (EOs) have become potential ingredients for insecticide formulations due to their widespread availability and perceived safety. Therefore, this study aimed to evaluate the toxicity and biochemical efficacy of basil (Ocimum basilicum) (Lamiaceae) against two destructive pests Noctuidae, Agrotis ipsilon (Hufnagel) and Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae). In addition, a molecular docking study was performed to gain insight into the binding pattern between glutathione S-transferase (GST) and linalool, the main component of EO. GC–MS analysis of O. basilicum EO revealed that linalool is the most abundant compound (29.34%). However, the toxicity tests showed no significant difference between the values of LC₅₀ of O. basilicum EO to A. ipsilon and S. littoralis. On the other hand, the sublethal experiments indicated that treating the second instar larvae with LC₁₅ or LC₅₀ values of O. basilicum EO significantly prolonged the larval duration in both insects, compared to the control. Regarding the biochemical effect of O. basilicum EO, the treatments significantly impacted the activity of detoxification enzymes. A notable elevation in glutathione S-transferase (GST) activity was recorded in A. ipsilon larvae compared with a reduction in S. littoralis larvae. The molecular docking analysis revealed that linalool bonded with the amino acid serine (SER 9) of GST, indicating its binding affinity with the enzyme. The obtained results could offer valuable insights into the mode of action of O. basilicum and can encourage the adoption of sustainable pest control practices that incorporate essential oils.

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实现可持续害虫管理：鳞翅目：夜蛾科）的毒性、生化作用和分子对接分析

摘要在过去十年中，精油（EOs）因其广泛的可获得性和可感知的安全性而成为杀虫剂配方的潜在成分。因此，本研究旨在评估罗勒（Ocimum basilicum）（唇形科）对两种破坏性害虫夜蛾科害虫 Agrotis ipsilon (Hufnagel) 和 Spodoptera littoralis (Boisduval) （鳞翅目：夜蛾科）的毒性和生化功效。此外，还进行了分子对接研究，以深入了解谷胱甘肽 S-转移酶（GST）与芳樟醇（环氧乙烷的主要成分）之间的结合模式。罗勒香根环氧乙烷的 GC-MS 分析表明，芳樟醇是含量最高的化合物（29.34%）。然而，毒性测试表明，紫苏环氧乙烷对 A. ipsilon 和 S. littoralis 的半数致死浓度没有显著差异。另一方面，亚致死实验表明，与对照组相比，用 LC15 或 LC50 值的 O. basilicum EO 处理第二龄幼虫可显著延长两种昆虫的幼虫期。关于 O. basilicum 环氧乙烷的生化效应，处理对解毒酶的活性有显著影响。A. ipsilon 幼虫的谷胱甘肽 S 转移酶（GST）活性明显提高，而 S. littoralis 幼虫的活性则有所降低。分子对接分析表明，芳樟醇与 GST 的氨基酸丝氨酸（SER 9）结合，表明其与该酶的结合亲和力。所获得的结果可为了解罗勒香豆素的作用模式提供有价值的见解，并可鼓励采用结合精油的可持续害虫控制方法。

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来源期刊

Neotropical Entomology 生物-昆虫学

CiteScore

3.30

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： Neotropical Entomology is a bimonthly journal, edited by the Sociedade Entomológica do Brasil (Entomological Society of Brazil) that publishes original articles produced by Brazilian and international experts in several subspecialties of entomology. These include bionomics, systematics, morphology, physiology, behavior, ecology, biological control, crop protection and acarology.