Meng Shan, Mengmeng Duan, Huimin Shen, Yujing Wang, Yiru Zhang, Xukai Li, Yuanhuai Han, Zhirong Yang, Kai Zhao, Xingchun Wang
{"title":"Identification and characterization of four novel xiaomi alleles to facilitate foxtail millet as a C4 model plant","authors":"Meng Shan, Mengmeng Duan, Huimin Shen, Yujing Wang, Yiru Zhang, Xukai Li, Yuanhuai Han, Zhirong Yang, Kai Zhao, Xingchun Wang","doi":"10.1007/s10725-024-01134-0","DOIUrl":null,"url":null,"abstract":"<p>A diverse genetic background is essential for genetic analysis and functional genomics research in model plants. In this study, four novel <i>xiaomi</i>-like mutants in different genetic backgrounds, named <i>xiaomi3</i>, <i>xiaomi4</i>, <i>xiaomi5</i>, and <i>xiaomi6</i>, were identified and characterized. These mutants exhibited an extremely early heading phenotype, with heading occurring around 30–40 days after sowing under natural long-day conditions. Significant reductions in plant height, leaf length, leaf width, panicle length, and panicle diameter were observed in the mutants compared to their corresponding wild-types. Notably, these mutants displayed diverse panicle architectures and hull colors, effectively preventing seed mixing between them. Subsequent investigation under controlled short-day and long-day conditions confirmed the significant early heading phenotype of these mutants. Molecular characterization revealed that mutations in the <i>Phytochrome C</i> (<i>SiPHYC</i>) gene, including transposon insertions and a frame shift mutation, were responsible for the extremely early heading phenotype. RNA-sequencing (RNA-Seq) analysis identified 19 differentially expressed genes associated with this phenotype. Additionally, genome-wide InDels and SNPs were identified, providing valuable resources for marker-assisted breeding and genetic studies. These findings will contribute to our understanding of the genetic and molecular mechanisms underlying <i>SiPHYC-</i>mediated photoperiod flowering, and provide valuable resources that will push <i>xiaomi</i> as a C<sub>4</sub> model plant.</p>","PeriodicalId":20412,"journal":{"name":"Plant Growth Regulation","volume":"104 1","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Growth Regulation","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10725-024-01134-0","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
A diverse genetic background is essential for genetic analysis and functional genomics research in model plants. In this study, four novel xiaomi-like mutants in different genetic backgrounds, named xiaomi3, xiaomi4, xiaomi5, and xiaomi6, were identified and characterized. These mutants exhibited an extremely early heading phenotype, with heading occurring around 30–40 days after sowing under natural long-day conditions. Significant reductions in plant height, leaf length, leaf width, panicle length, and panicle diameter were observed in the mutants compared to their corresponding wild-types. Notably, these mutants displayed diverse panicle architectures and hull colors, effectively preventing seed mixing between them. Subsequent investigation under controlled short-day and long-day conditions confirmed the significant early heading phenotype of these mutants. Molecular characterization revealed that mutations in the Phytochrome C (SiPHYC) gene, including transposon insertions and a frame shift mutation, were responsible for the extremely early heading phenotype. RNA-sequencing (RNA-Seq) analysis identified 19 differentially expressed genes associated with this phenotype. Additionally, genome-wide InDels and SNPs were identified, providing valuable resources for marker-assisted breeding and genetic studies. These findings will contribute to our understanding of the genetic and molecular mechanisms underlying SiPHYC-mediated photoperiod flowering, and provide valuable resources that will push xiaomi as a C4 model plant.
期刊介绍:
Plant Growth Regulation is an international journal publishing original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research using hormonal, physiological, environmental, genetical, biophysical, developmental or molecular approaches to the study of plant growth regulation.
Emphasis is placed on papers presenting the results of original research. Occasional reviews on important topics will also be welcome. All contributions must be in English.