Identification and characterization of microRNAs in virus-resistant and susceptible barley cultivars

IF 3.5 3区 生物学 Q1 PLANT SCIENCES Plant Growth Regulation Pub Date : 2024-09-11 DOI:10.1007/s10725-024-01211-4
Mengna Zhang, Yi Hong, Lufei Li, Juan Zhu, Chao Lv, Baojian Guo, Feifei Wang, Rugen Xu
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Abstract

Barley (Hordeum vulgare L.) is the fourth most productive cereal crop in the world, but barley sown in winter is often infected by the barley yellow mosaic virus (BaYMV) and the barley mild mosaic virus (BaMMV), which cause mosaic, stunting, discoloration and yield reduction. Plant microRNA (miRNA) plays a major part in defense against viral infection. In this study, total sRNAs from the leaves of three barley cultivars infected with a soil-borne virus, BaYMV or BaMMV, were analyzed by RNA sequencing. A total of 35 known miRNAs and 70 novel miRNAs were identified, of which hvu-miR397a and huv-miR156a were most differentially expressed. A total of 18 miRNAs were found to target 259 genes. Enrichment analysis revealed that targeted genes were associated with chloroplast functions. Overall, the results provided a whole landscape of small RNAs and mRNAs in response to BaYMV infection and can be referred to in plant breeding against virus infection.

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抗病毒和易感大麦栽培品种中 microRNA 的鉴定和表征
大麦(Hordeum vulgare L.)是世界上第四大高产谷物作物,但冬播大麦经常受到大麦黄花叶病毒(BaYMV)和大麦轻度花叶病毒(BaMMV)的感染,这两种病毒会导致大麦马赛克、发育不良、变色和减产。植物微 RNA(miRNA)在抵御病毒感染方面发挥着重要作用。本研究通过 RNA 测序分析了感染土传病毒 BaYMV 或 BaMMV 的三种大麦栽培品种叶片的总 sRNA。共鉴定出 35 个已知 miRNA 和 70 个新型 miRNA,其中 hvu-miR397a 和 huv-miR156a 的表达差异最大。共发现 18 个 miRNA 靶向 259 个基因。富集分析表明,靶基因与叶绿体功能有关。总之,研究结果提供了小RNA和mRNA对BaYMV感染的整体响应图谱,可用于植物育种以抵御病毒感染。
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来源期刊
Plant Growth Regulation
Plant Growth Regulation 生物-植物科学
CiteScore
6.90
自引率
9.50%
发文量
139
审稿时长
4.5 months
期刊介绍: Plant Growth Regulation is an international journal publishing original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research using hormonal, physiological, environmental, genetical, biophysical, developmental or molecular approaches to the study of plant growth regulation. Emphasis is placed on papers presenting the results of original research. Occasional reviews on important topics will also be welcome. All contributions must be in English.
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