Unveiling the tripartite synergistic interaction of plant-arbuscular mycorrhizal fungus symbiosis by endophytic Bacillus velezensis S141 in Lotus japonicus

IF 2.1 3区生物学 Q3 MICROBIOLOGY Symbiosis Pub Date : 2024-03-11 DOI:10.1007/s13199-024-00975-7

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Abstract

The crucial roles played by arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR) in enhancing plant nutrient uptake and soil quality are widely recognized across various plant species. This study explored the effects and potential of Bacillus velezensis S141 as a plant growth-promoting rhizobacterium on promoting a symbiotic relationship of AMF, Rhizophagus irregularis with Lotus japonicus. B. velezensis S141 inoculation positively influenced fungal growth and development. B. velezensis S141 promoted fungal abundance, such as AM root colonization and spore number. It also boosted plant nutrient uptake, enhancing the nitrogen and phosphorus concentration by 1.65 and 1.51 times, respectively, under tripartite interaction conditions. However, the indole-3-acetic acid (IAA) producing capability of B. velezensis S141, based on the inoculation experiment test of S141 mutants defective in IAA synthesis, was not the key mechanism for promoting this symbiotic interaction. Interestingly, the S141 strain, originating from rhizospheric soil fields of soybeans, was found to penetrate plant root cells and establish itself as an endophyte. The presence of B. velezensis S141 not only triggered the expression of marker genes associated with early stages of AMF colonization and nutrient uptake in the host plant, but it also led to an upregulation of AMF genes responsible for cell cycle regulation. These results suggest that B. velezensis S141 holds promise as a helper bacterium in promoting plant-AMF symbiosis.

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内生芽孢杆菌 S141 在日本莲中揭示植物与丛枝菌根真菌共生的三方协同作用

摘要树胶菌根真菌（AMF）和植物生长促进根瘤菌（PGPR）在提高植物养分吸收和土壤质量方面发挥着重要作用，这一点已在各种植物物种中得到广泛认可。本研究探讨了芽孢杆菌 S141 作为植物生长促进根瘤菌对促进 AMF、Rhizophagus irregularis 与日本莲共生关系的影响和潜力。接种 B. velezensis S141 对真菌的生长和发育有积极影响。B. velezensis S141 促进了真菌丰度，如 AM 根定植和孢子数量。它还促进了植物对养分的吸收，在三方作用条件下，氮和磷的浓度分别提高了 1.65 倍和 1.51 倍。然而，根据对 IAA 合成缺陷的 S141 突变体的接种实验测试，B. velezensis S141 产生吲哚-3-乙酸（IAA）的能力并不是促进这种共生相互作用的关键机制。有趣的是，S141 菌株来自大豆的根瘤土壤田，它能穿透植物根细胞，成为植物的内生菌。B. velezensis S141 的存在不仅引发了与 AMF 定殖早期阶段和宿主植物养分吸收相关的标记基因的表达，还导致了负责细胞周期调控的 AMF 基因的上调。这些结果表明，B. velezensis S141有望成为促进植物-AMF共生的辅助细菌。

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来源期刊

Symbiosis 生物-微生物学

CiteScore

4.80

自引率

8.00%

发文量

审稿时长

>12 weeks

期刊介绍： Since 1985, Symbiosis publishes original research that contributes to the understanding of symbiotic interactions in a wide range of associations at the molecular, cellular and organismic level. Reviews and short communications on well-known or new symbioses are welcomed as are book reviews and obituaries. This spectrum of papers aims to encourage and enhance interactions among researchers in this rapidly expanding field. Topics of interest include nutritional interactions; mutual regulatory and morphogenetic effects; structural co-adaptations; interspecific recognition; specificity; ecological adaptations; evolutionary consequences of symbiosis; and methods used for symbiotic research.