Effects of deltamethrin exposure on the cytochrome P450 monooxygenases of Aedes albopictus (Skuse) larvae from a dengue-endemic region of northern part of West Bengal, India

IF 1.6 3区农林科学 Q2 ENTOMOLOGY Medical and Veterinary Entomology Pub Date : 2024-03-13 DOI:10.1111/mve.12713

Prapti Das, Subhajit Das, Abhirup Saha, Debayan Raha, Dhiraj Saha

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Abstract

Aedes albopictus is highly prevalent in the northern part of West Bengal and is considered to be responsible for the recent dengue outbreaks in this region. Control of this vector is largely relied on the use of synthetic pyrethroids, which can lead to the development of resistance. In the present study, larvae of three wild Ae. albopictus populations from the dengue-endemic regions were screened for deltamethrin resistance, and the role of cytochrome P450 monooxygenases (CYPs) was investigated in deltamethrin exposed and unexposed larvae. Two populations were incipient resistant, and one population was completely resistant against deltamethrin. Monooxygenase titration assay revealed the involvement of CYPs in deltamethrin resistance along with an induction effect of deltamethrin exposure. Gene expression studies revealed differential expression of five CYP6 family genes, CYP6A8, CYP6P12, CYP6A14, CYP6N3 and CYP6N6, with high constitutive expression of CYP6A8 and CYP6P12 in all the populations before and after deltamethrin exposure. From these findings, it was evident that CYPs play an important role in the development of deltamethrin resistance in the Ae. albopictus populations in this region.

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暴露于溴氰菊酯对印度西孟加拉邦北部登革热流行地区白纹伊蚊（Skuse）幼虫细胞色素 P450 单氧化酶的影响。

白纹伊蚊在西孟加拉邦北部地区非常普遍，被认为是该地区最近爆发登革热的罪魁祸首。对这种病媒的控制主要依赖于合成除虫菊酯的使用，这可能会导致抗药性的产生。在本研究中，对登革热流行地区的三个野生白纹伊蚊种群的幼虫进行了溴氰菊酯抗药性筛选，并研究了暴露于溴氰菊酯和未暴露于溴氰菊酯的幼虫体内细胞色素 P450 单氧化酶（CYPs）的作用。两个种群对溴氰菊酯具有初抗性，一个种群对溴氰菊酯具有完全抗性。单氧化酶滴定测定显示，CYPs参与了溴氰菊酯抗性的产生，同时暴露于溴氰菊酯会产生诱导效应。基因表达研究揭示了五个 CYP6 家族基因（CYP6A8、CYP6P12、CYP6A14、CYP6N3 和 CYP6N6）的差异表达，在接触溴氰菊酯前后，所有种群中的 CYP6A8 和 CYP6P12 都有较高的组成型表达。这些发现表明，CYPs 在该地区白纹伊蚊种群对溴氰菊酯产生抗性的过程中发挥了重要作用。

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来源期刊

Medical and Veterinary Entomology 农林科学-昆虫学

CiteScore

3.70

自引率

5.30%

发文量

审稿时长

12-24 weeks

期刊介绍： Medical and Veterinary Entomology is the leading periodical in its field. The Journal covers the biology and control of insects, ticks, mites and other arthropods of medical and veterinary importance. The main strengths of the Journal lie in the fields of: -epidemiology and transmission of vector-borne pathogens changes in vector distribution that have impact on the pathogen transmission- arthropod behaviour and ecology- novel, field evaluated, approaches to biological and chemical control methods- host arthropod interactions. Please note that we do not consider submissions in forensic entomology.